-
Bulked Segregant analysis
Bulked segregant analysis (BSA) is a technique employed to quickly identify phenotype associated genetic markers. Main workflow of BSA contains selecting two groups of individuals with extremely opposing phenotypes, pooling the DNA of all individuals to form two bulk of DNA, identifying differential sequences between two pools. This technique has been extensively employed in identifying genetic markers strongly associated by targeted genes in plant/animal genomes.
-
Comparative Genomics
Comparative genomics literally means comparing the complete genome sequences and structures of different species. This discipline aims at revealing species evolution, gene function, gene regulatory mechanism at genome level by identifying the sequence structures and elements that conserved or differentiated across different species. Typical comparative genomics study includes analyses in gene family, evolutionary development, whole genome duplication, selective pressure, etc.
-
Evolutionary Genetics
Population and evolutionary genetic analysis platform is established on base of massive experience accumulated within BMK R&D team for years. It is a user friendly tool especially for researchers who are not majoring in bioinformatics. This platform enables basic evolutionary genetics related basic analysis including phylogenetic tree construction, linkage disequilibrium analysis, genetic diversity assessment, selective sweep analysis, kinship analysis, PCA, population structure analysis, etc.
-
Hi-C based Genome Assembly
Hi-C is a method designed to capture chromosome configuration by combining probing proximity-based interactions and high-throughput sequencing. The intensity of these interactions is believed to be negatively correlated with physical distance on chromosomes. Therefore, Hi-C data could guide the clustering, ordering and orienting of assembled sequences in a draft genome and anchoring those onto a certain number of chromosomes. This technology empowers a chromosome-level genome assembly in absence of population-based genetic map. Every single genome needs a Hi-C.
Platform：Illumina NovaSeq6000 / DNBSEQ
-
Plant/Animal De novo Genome Sequencing
De novo sequencing refers to construction of a species’ whole genome using sequencing technologies, e.g. PacBio, Nanopore, NGS, etc., in absence of a reference genome. The remarkable improvement in read length of third generation sequencing technologies has brought new opportunities in assembling complex genomes, such as those with high heterozygosity, high ratio of repetitive regions, polyploids, etc. With read length at tens of kilobases level, these sequencing reads enable resolving of repetitive elements, regions with abnormal GC contents and other highly complex regions.
Platform： PacBio Sequel II /Nanopore PromethION P48/ Illumina NovaSeq6000
-
Human Whole Exome Sequencing
Whole exome sequencing (WES) is regarded as a cost-effective sequencing strategy for identifying disease-causing mutations. Although exons only take up approximately 1.7% of entire genome, it represents the profile of total protein functions directly. In human genome, it has been reported that more than 85% of disease related mutations occur in protein coding region.
BMKGENE offers comprehensive and flexible human whole exome sequencing services with different exon capturing strategies available to meet various research goals.
Platform: Illumina NovaSeq 6000
-
Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, especially on large-scale population, is a fundamental step in genetic association studies, which provides genetic basis for functional gene discovery, evolutionary analysis, etc. Instead of deep whole genome re-sequencing, reduced representation genome sequencing (RRGS) is introduced to minimize sequencing cost per sample, while maintain reasonable efficiency on genetic marker discovery. This is commonly achieved by extracting restriction fragment within given size range, which is named reduced representation library (RRL). Specific-locus amplified fragment sequencing (SLAF-Seq) is a self-developed strategy for SNP genotyping with or without a reference genome.
Platform: Illumina NovaSeq 6000 -
DNA/RNA Sequencing – Nanopore Sequencer
ONT sequencing is a single molecule real-time electrical signal sequencing technology based on nanopores, the sequencing principle of each platform is the same. Double-stranded DNA/RNA will bind to nanoporous protein embedded in the biofilm and unwinding under the lead of motor protein, under the action of voltage difference from both sides of the biofilm, DNA/RNA strands pass through the nanopore channel protein at a certain rate. Due to the differences of the chemical properties of the different bases on the DNA/RNA strand, when a single base or DNA molecule passes through the nanopore channel, it will cause the change of different electrical signals. By detecting and corresponding to these signals, the corresponding base types can be calculated, and the real-time detection of the sequence can be completed.
-
DNA/RNA Sequencing – Illumina Sequencer
Illumina sequencing technology, sequencing by synthesis (SBS), is a widely adopted next-generation sequencing (NGS) technology worldwide, responsible for generating more than 90% of the world’s sequencing data. A fluorescently labeled reversible terminator is imaged as each dNTP is added, and then cleaved to allow incorporation of the next base. Since all 4 reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias. The method virtually eliminates errors and missed calls associated with strings of repeated nucleotides (homopolymers). Illumina sequencing by synthesis technology supports both single-read and paired-end libraries. SBS technology offers a short-insert paired-end capability for high-resolution genome sequencing, as well as long-insert paired-end reads for efficient sequence assembly, de novo sequencing, and more. The combination of short inserts and longer reads increases the ability to fully characterize any genome.
-
DNA/RNA sequencing -PacBio Sequencer
PacBio sequencing platform is a long-read sequencing platform, which is also known as one of the Third-Generation Sequencing(TGS) technologies. The core technology, single-molecule real-time(SMRT), empowers the generation of reads with tens of kilo-base in length. On base of “Sequencing-by-Synthesis”, single nucleotide resolution is achieved by Zero-mode waveguide(ZMW), where only limited volume at the bottom(site of molecule synthesis), is illuminated. In addition, SMRT sequencing largely avoids sequence-specific bias in NGS system, in that most of PCR amplification steps are not required in library construction process.
Platform: Sequel II, Revio
