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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
This method developed independently by BMKGene, can be classified inside the reduced representation genome sequencing. It optimizes the restriction enzyme set for every project. This ensures the generation of a substantial number of SLAF tags (400-500 bps regions of the genome being sequenced) that are uniformly distributed across the genome while effectively avoiding repetitive regions, thus assuring the best genetic marker discovery.
It provides a fast genotyping and sets the basis for functional gene discovery or evolutionary analysis reducing the cost per sample while maintaining the efficiency in genetic marker discovery. RRGS achieves this by digesting DNA with restriction enzymes and focusing on a specific fragment size range, thereby sequencing only a fraction of the genome. Among the various RRGS methodologies, Specific-Locus Amplified Fragment Sequencing (SLAF) is a customizable and high-quality approach.
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Illumina pre-made libraries
Illumina sequencing technology, based on Sequencing by Synthesis (SBS), is a globally embraced NGS innovation, responsible for generating over 90% of the world’s sequencing data. The principle of SBS involves imaging fluorescently labelled reversible terminators as each dNTP is added, and subsequently cleaved to allow the incorporation of the next base. With all four reversible terminator-bound dNTPs present in each sequencing cycle, natural competition minimizes incorporation bias. This versatile technology supports both single-read and paired-end libraries, catering to a range of genomic applications. Illumina sequencing’s high-throughput capabilities and precision position it as a cornerstone in genomics research, empowering scientists to unravel the intricacies of genomes with unmatched detail and efficiency.
Our pre-made library sequencing service enables customers to prepare sequencing libraries from diverse sources (mRNA, whole genome, amplicon, 10x libraries, among others). Subsequently, these libraries can be shipped to our sequencing centers for quality control and sequencing in Illumina platforms.
