kuzenguruka RNA ikurikirana-Illumina
Inyungu za serivisi
ØCircRNA ifitanye isano cyane na mRNA imvugo igenga
ØYagize uruhare muri sisitemu yo kugenzura circRNA - miRNA - mRNA
ØKumenyekanisha hamwe na statistique kumuzingo uzwi RNA hamwe nudushya twa circRNA
ØBMKCloud ishingiye kubisubizo: Gutanga amakuru yihariye kubucukuzi buboneka kurubuga.
ØSerivise nyuma yo kugurisha ifite agaciro kumezi 3 iyo umushinga urangiye
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
Nucleotide:
| Isuku | Ubunyangamugayo | Kwanduza | Umubare |
| OD260 / 280≥1.7-2.5;OD260 / 230≥0.5-2.5; | Kubimera: RIN≥6.5;Ku nyamaswa: RIN≥7;28S / 18S≥1.0;bigarukira cyangwa bidafite ishingiro | Ntarengwa cyangwa ntayo poroteyine cyangwa ADN yanduye kuri gel. | Umwanzuro.≥100ng / μl;Inkingi ≥ 10 μl;Igiteranyo ≥2μg |
Tissue: Uburemere (bwumye): ≥1 g
* Kuri tissue ntoya ya mg 5, turasaba kohereza flash ya flash ikonje (muri azote yuzuye).
Guhagarika ingirabuzimafatizo: Kubara Akagari = 3 × 107
* Turasaba kohereza lysate ya selile ikonje.Mugihe iyo selile ibara ntoya 5 × 105, flash yahagaritswe muri azote yuzuye.
Ingero zamaraso:
PA×geneBrRNATube;
6mLTRIzol n'amaraso 2mL (TRIzol:Amaraso= 3: 1)
Basabwe Gutanga Icyitegererezo
Ibikoresho: ml 2 ya centrifuge (Tin foil ntabwo isabwa)
Icyitegererezo cyerekana: Itsinda + kwigana urugero A1, A2, A3;B1, B2, B3 ... ...
Kohereza:
Urubura rwumye: Ingero zigomba gupakirwa mumifuka hanyuma zigashyingurwa mu rubura.
Imiyoboro ya RNA: Ingero za RNA zirashobora gukama mumashanyarazi ya RNA (urugero RNAstable®) hanyuma ikoherezwa mubushyuhe bwicyumba.
Akazi ka serivisi
Igishushanyo mbonera
Icyitegererezo
Gukuramo RNA
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
Bioinformatics
 |
1.CircRNA
Byaravuzwe ko ubwinshi bwa circRNAs ikomoka kuri exons aho kuba intore cyangwa uturere.Byongeye kandi gukwirakwiza circRNA iratandukanye cyane muri chromosomes.Kubwibyo, ni iby'agaciro gakomeye gusuzuma isaranganya ritandukanye rya circRNA yakiriye hagati yintangarugero zitandukanye, harimo no gukwirakwiza uturere twa gene zitandukanye no kuri chromosomes zitandukanye.
Ikwirakwizwa rya circRNA
2.Gusesengura Isesengura kuri DE-circRNA
Ihuriro ryimiterere ya circRNAs itandukanye
3.GO Ibisobanuro kuri DE-circRNA Intangiriro Intangiriro
Genda gutondekanya DE-circRNA yakira gen
Urubanza rwa BMK
CPSF4 igenga imiterere ya circRNA na microRNA yunganiwe gucecekesha kanseri ya hepatocellular
Byatangajwe: Oncogene, 2021
Ibisubizo by'ingenzi
1.Imvugo ya CPSF4 iteganijwe muri HCC kandi iyo mvugo yo hejuru ya CPSF4 ifitanye isano no kutamenya neza abarwayi ba HCC.
2
3.Ubushakashatsi mumico ya selile na xenograft yimbeba yerekana ko CPSF4 iteza imbere ikwirakwizwa rya selile HCC kandi ikongera tumorigenicity.Byongeye kandi, CPSF4 irwanya ibibyimba byo gukuramo ibibyimba byo hepfo ya RNA muri HCC
 urwego rwa miRNA n'urwego rwa poroteyine |
Wang X, Dong J, Li X, n'abandi.CPSF4 igenga imiterere ya circRNA hamwe na microRNA ihuza gucecekesha kanseri ya hepatocellular [J].Oncogene.DOI ： 10.1038 / s41388-021-01867-6
