Umwanya muremure utari code-Illumina
Inyungu za serivisi
ØInyungu za serivisi
ØIngirabuzimafatizo na tissue byihariye
ØIcyiciro cyihariye kigaragaza kandi kigaragaza imvugo ihinduka
ØUburyo bwiza bwigihe nigihe cyo kwerekana
ØIsesengura rifatanije namakuru ya mRNA.
ØBMKCloud ishingiye kubisubizo: Gutanga amakuru yihariye kubucukuzi buboneka kurubuga.
ØSerivise nyuma yo kugurisha ifite agaciro kumezi 3 iyo umushinga urangiye
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
Nucleotide:
Tissue: Uburemere (bwumye): ≥1 g
* Kuri tissue ntoya ya mg 5, turasaba kohereza flash ya flash ikonje (muri azote yuzuye).
Guhagarika ingirabuzimafatizo: Kubara Akagari = 3 × 107
* Turasaba kohereza lysate ya selile ikonje.Mugihe iyo selile ibara ntoya 5 × 105, flash yahagaritswe muri azote yuzuye.
Ingero zamaraso:
PA × geneBloRNRNube;
6mLTRIzol n'amaraso 2mL (TRIzol: Amaraso = 3: 1)
Basabwe Gutanga Icyitegererezo
Ibikoresho: ml 2 ya centrifuge (Tin foil ntabwo isabwa)
Icyitegererezo cyerekana: Itsinda + kwigana urugero A1, A2, A3;B1, B2, B3 ... ...
Kohereza:
1.Ibara ryumye: Ingero zigomba gupakirwa mumifuka hanyuma zigashyingurwa mu rubura.
3.Imiyoboro ihamye: Ingero za RNA zirashobora gukama mumashanyarazi ya RNA (urugero RNAstable®) hanyuma ikoherezwa mubushyuhe bwicyumba.
Akazi ka serivisi
Igishushanyo mbonera
Icyitegererezo
Gukuramo RNA
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
Bioinformatics
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1.LncRNA itondekanya
LncRNA yahanuwe na software enye yavuzwe haruguru yashyizwe mubyiciro 4: lincRNA, anti-sense-LncRNA, intronic-LncRNA;ibisobanuro-LncRNA.Ibyiciro bya LncRNA byerekanwe muri histogramu hepfo.
Ibyiciro bya LncRNA
2.Cis-genes igenewe DE-lncRNA isesengura ryitunganywa
ClusterProfiler yakoreshwaga mu isesengura ryitunganyirizwa rya GO kuri cis-igenewe gen zigaragazwa mu buryo butandukanye lncRNA (DE-lncRNA), mubijyanye na biologiya, imikorere ya molekile nibigize selile.GO isesengura ryitunganyirizo ni inzira yo kumenya DEG iyobowe cyane na GO ugereranije na genome yose.Amagambo akungahaye yerekanwe muri histogramu, imbonerahamwe ya bubble, nibindi nkuko bigaragara hano hepfo.
Cis-igenewe genes ya DE-lncRNA isesengura ryitunganywa -Imbonerahamwe
Urubanza rwa BMK
Kugaragaza imiterere ya lncRNA mumashusho yimbeba adenocarcinoma hamwe na KRAS - G12D mutation na P53 knockout.
Byatangajwe:Ikinyamakuru c'Ubuvuzi bwa Cellular na Molecular ，2019
Ingamba zikurikirana
Illumina
Icyegeranyo cy'icyitegererezo
NONMMUT015812-knockdown KP (shRNA-2) selile na selile mbi (sh-Scr) byabonetse kumunsi wa 6 wanduye virusi.
Ibisubizo by'ingenzi
Ubu bushakashatsi bukora iperereza kuri lncRNAs mu mbeba y'ibihaha adenocarcinoma hamwe na P53 knockout na mutation ya KrasG12D.
1.6424 lncRNAs zagaragajwe muburyo butandukanye (change Guhindura inshuro 2, P <0.05).
2.Mu 210 lncRNAs zose (FC≥8), imvugo ya lncRNAs 11 yagengwaga na P53, 33 lncRNAs na KRAS na 13 lncRNAs na hypoxia muma selile yibanze ya KP.
3.NONMMUT015812, yagenzuwe kuburyo budasanzwe mu mbeba y'ibihaha adenocarcinoma kandi ikagengwa na P53 yongeye kwerekana, byagaragaye kugirango isesengure imikorere yayo.
4.Gukomanga kuri NONMMUT015812 na shRNAs byagabanije gukwirakwiza no kwimuka kwimikorere ya selile.NONMMUT015812 yari oncogene ishobora kuba.
 Inzira ya KEGG isesengura genes zitandukanye muburyo bwa NONMMUT015812-gukubita KP selile |  Gene Ontology isesengura ingirabuzimafatizo zitandukanye muri NONMMUT015812-gukubita KP selile |
Reba
Kugaragaza imiterere ya lncRNA mumashusho yimbeba adenocarcinoma hamwe na KRAS - G12D ihindagurika na P53 knockout [J].Ikinyamakuru c'Ubuvuzi bwa Cellular na Molecular, 2019, 23 (10).DOI ： 10.1111 / jcmm.14584
