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Ibicuruzwa

Urutonde rwa Chromatin Immunoprecipitation (ChIP-seq)

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Featured Image
  • Chromatin Immunoprecipitation Sequencing (ChIP-seq)

ChIP-Seq itanga genome-yerekana ishusho ya ADN yo guhindura amateka, ibintu byandikirwa, hamwe na poroteyine zijyanye na ADN.Ihuza uburyo bwo guhitamo chromatin immuno-imvura (ChIP) kugirango igarure poroteyine-ADN yihariye, hamwe nimbaraga zo gukurikiraho kuzakurikiraho (NGS) kugirango bikurikirane neza ADN yagaruwe.Byongeye kandi, kubera ko poroteyine-ADN igarurwa mu ngirabuzimafatizo, ibibanza bihuza bishobora kugereranywa mu bwoko butandukanye no mu ngingo zitandukanye, cyangwa mu bihe bitandukanye.Porogaramu iratandukanye kuva amabwiriza yo kwandikirana kugeza inzira yiterambere kugeza uburyo bwindwara ndetse nibindi.

Ihuriro: Illumina NovaSeq 6000


Ibisobanuro bya serivisi

Inyungu za serivisi

ØIhuriro: NovaSeq 6000 platform, PE150

ØUbwoko bw'isomero: 100-500bp shyiramo isomero rya cDNA (biterwa no gukwirakwiza impinga)

ØIngamba zikurikirana: Byombi-Impera 150 bp

ØSaba amakuru asohoka: 6 Gb amakuru yibanze / icyitegererezo.

Icyitegererezo

Ingano ya ADN: ng 70 ng, impinga nyamukuru ya 100 kugeza 500 bp

Ingano yicyitegererezo: ≥ 10 μl

OD260 / 280 = 1.8 kugeza 2.0 nta kwangirika cyangwa kwanduza RNA

Akazi ka serivisi

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Icyitegererezo

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Kubaka isomero

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Urukurikirane

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Isesengura ryamakuru

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