Urutonde rwose rwanditse - Illumina
Inyungu za serivisi
ØKugereranya kubwoko bwose bwa RNAs mubare, kubara, imvugo ya chromosome
ØKumenyekanisha RNAs zitandukanye kandi imvugo ihuye
ØIsesengura rya Gene hamwe
Øisesengura ry'urusobe rwa ceRNA
ØIngirabuzimafatizo zingenzi zirimo gusesengura inzira
ØBMKCloud ishingiye kubisubizo: Gutanga amakuru yihariye kubucukuzi buboneka kurubuga.
ØSerivise nyuma yo kugurisha ifite agaciro kumezi 3 iyo umushinga urangiye
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
Nucleotide:
| Isuku | Ubunyangamugayo | Kwanduza | Umubare |
| OD260 / 280≥1.7-2.5 ； OD260 / 230≥0.5-2.5 ； | Kubimera: RIN≥6.5;Ku nyamaswa: RIN≥7;28S / 18S≥1.0;bigarukira cyangwa bidafite ishingiro | Ntarengwa cyangwa ntayo poroteyine cyangwa ADN yanduye kuri gel. | Umwanzuro.≥100 ng / μl;Inkingi ≥ 10 μl;Igiteranyo ≥ 2 μg |
Tissue: Uburemere (bwumye): ≥1 g
* Kuri tissue ntoya ya mg 5, turasaba kohereza flash ya flash ikonje (muri azote yuzuye).
Guhagarika ingirabuzimafatizo: Kubara Akagari = 3 × 107
* Turasaba kohereza lysate ya selile ikonje.Mugihe iyo selile ibara munsi ya 5 × 105, birashoboka ko flash ikonjeshwa muri azote yuzuye.
Ingero zamaraso:
PA × geneBloRNRNube;
6mLTRIzol n'amaraso 2mL (TRIzol: Amaraso = 3: 1)
Basabwe Gutanga Icyitegererezo
Ibirimwo:
2 ml centrifuge tube (Tin foil ntabwo isabwa)
Icyitegererezo cyerekana: Itsinda + kwigana urugero A1, A2, A3;B1, B2, B3 ... ...
Kohereza:
1.Ibara ryumye: Ingero zigomba gupakirwa mumifuka hanyuma zigashyingurwa mu rubura.
2.Imiyoboro ihamye: Ingero za RNA zirashobora gukama mumashanyarazi ya RNA (urugero RNAstable®) hanyuma ikoherezwa mubushyuhe bwicyumba.
Akazi ka serivisi
Igishushanyo mbonera
Icyitegererezo
Gukuramo RNA
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
Bioinformatics
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1.Gusuzuma ubwoko bwose bwa RNA bushingiye kumvugo
Circos ku kamaro ko gutandukanya imvugo ya RNA
2.Ihuriro ryinzira ya KEGG
Umuyoboro wa KEGG wuzuye
3.isesengura ryurusobe
ceRNA Umuyoboro ushingiye kuri DE-circRNA-miRNA-mRNA Imikoranire
Urubanza rwa BMK
Inzira ya CircRNA yerekana na ceRNA na miRNA - mRNA Imiyoboro Yagize uruhare mugutezimbere Ibindi muri CMS Umurongo wa Brassica campestris
Byatangajwe:Ikinyamakuru mpuzamahanga cyubumenyi bwa molecular ，2019
NONMMUT015812-knockdown KP (shRNA-2) selile na selile mbi (sh-Scr) byabonetse kumunsi wa 6 wanduye virusi.
Ibisubizo by'ingenzi
Ubu bushakashatsi bwashyizeho umurongo wa Polima cytoplasm sterility (CMS) umurongo “Bcpol97-05A”, n'umurongo urumbuka, “Bcajh97-01B”, muri Brassica campestris L. ssp.chinensis Makino, syn.B. rapa ssp.chinensis, kandi ikora imvugo ya RNA igereranya hagati yindabyo zumurongo wa sterile numurongo urumbuka ukurikije inyandiko-mvugo yose.
1.Bose hamwe 31 bagaragaje mu buryo butandukanye (DE) circRNAs, 47 DE miRNAs, na 4779 DE mRNAs.
2.Isesengura rya gene ontologiya (GO) ryerekanye ko genes nyinshi za DE zagize uruhare mugutezimbere urukuta
3.Isesengura ryitunganyirizwa ryinzira ya KEGG ya DE mRNAs (harimo na mRNAs igenzurwa kandi igenzurwa na mRNAs kumurongo ugereranije nuburumbuke) yerekanaga ko "krahisi na sucrosemetabolism", "phenylpropanoid biosynthesis", na "pentose na glucuronate interconversions ”Arethe inzira ikungahaye cyane.
 Isesengura ryinzira ya KEGG ya mRNAs itandukanye |  Gene ontology (GO) itondekanya mRNAs itandukanye |  Reba ya DEcircRNA - DEmiRNA - DEmRNA umuyoboro wa gatatu |
Reba
Liang Y, Zhang Y, Xu L, n'abandi.Inzira ya CircRNA hamwe na ceRNA na miRNA - mRNA Imiyoboro Yagize uruhare mugutezimbere Ibindi mumurongo wa CMS kumurongo wa Brassica campestris [J].Ikinyamakuru mpuzamahanga cya siyansi yubumenyi, 2019, 20 (19): 4808-.DOI ： 10.3390 / ijms20194808
