Ubushinwa Eukaryotic mRNA ikurikirana-Uruganda rwa Illumina nababikora |BMK

Eukaryotic mRNA ikurikirana-Illumina

Eukaryotic mRNA sequencing-Illumina Featured Image

mRNA ikurikirana ituma umwirondoro wa mRNAs zose zandukuwe kuva selile mubihe byihariye.Nubuhanga bukomeye bwo kwerekana imiterere ya gene, imiterere ya gene hamwe na molekuline yuburyo bumwe na bumwe bwibinyabuzima.Kugeza ubu, urutonde rwa mRNA rwakoreshejwe cyane mubushakashatsi bwibanze, gusuzuma amavuriro, guteza imbere ibiyobyabwenge, nibindi.

Ihuriro: Illumina NovaSeq 6000

Twandikire

Ibisobanuro bya serivisi

Bioinformatics

Ibisubizo bya Demo

Ibyiza

ØInararibonye cyane: Ingero zirenga 200.000 zatunganijwe muri BMK zikubiyemo ubwoko butandukanye bw'icyitegererezo, harimo umuco w'utugari, ingirangingo, amazi yo mu mubiri, n'ibindi kandi imishinga irenga 7,000 mRNA-Seq ifunze ikubiyemo ahantu hatandukanye ubushakashatsi.

ØSisitemu yo kugenzura ubuziranenge bukomeye: Ingingo zingenzi zo kugenzura ubuziranenge binyuze mu ntambwe zose zirimo gutegura icyitegererezo, gutegura isomero, uko bikurikirana hamwe na bioinformatics birakurikiranirwa hafi kugirango bitange ibisubizo byiza.

ØUbubikoshingiro bwinshi buraboneka kubikorwa byo gutangaza no gutezimbere kugirango ugere ku ntego zitandukanye zubushakashatsi.

ØSerivise nyuma yo kugurisha: Serivisi nyuma yo kugurisha ifite agaciro mumezi 3 iyo umushinga urangiye, harimo imishinga ikurikirana, kurasa ibibazo, ibisubizo Q&A, nibindi.

Icyitegererezo gisabwa no gutanga

Isomero	Ingamba zikurikirana	Ibisobanuro byatanzwe	Kugenzura ubuziranenge
Poly A ikungahaye	Illumina PE150	6 Gb	Q30≥85%

Icyitegererezo gisabwa:

Nucleotide:

Isuku	Ubunyangamugayo	Umubare
OD260 / 280≥1.7-2.5 OD260 / 230≥0.5-2.5Ntarengwa cyangwa ntayo poroteyine cyangwa ADN yanduye kuri gel.	Kubimera: RIN≥6.5; Ku nyamaswa: RIN≥7; 28S / 18S≥1.0;	Umwanzuro.≥30 ng / μl;Inkingi ≥ 10 μl;Igiteranyo ≥ 1.5 μg

Tissue: Uburemere (bwumye):≥1 g
* Kuri tissue ntoya ya mg 5, turasaba kohereza flash ya flash ikonje (muri azote yuzuye).

Guhagarika akagari:Kubara Akagari = 3 × 10⁶- 1 × 10⁷
* Turasaba kohereza lysate ya selile ikonje.Mugihe iyo selile ibara ntoya ya 5 × 105, birashoboka ko flash ikonjeshwa muri azote yuzuye, bikaba byiza gukuramo mikoro.

Ingero zamaraso:Umubumbe wa ml

Microorganism:Misa ≥ 1 g

Basabwe Gutanga Icyitegererezo

Ibikoresho: ml 2 ya centrifuge (Tin foil ntabwo isabwa)

Icyitegererezo cyerekana: Itsinda + kwigana urugero A1, A2, A3;B1, B2, B3 ... ...

Kohereza:

Urubura rwumye: Ingero zigomba gupakirwa mumifuka hanyuma zigashyingurwa mu rubura.
Imiyoboro ya RNA: Ingero za RNA zirashobora gukama mumashanyarazi ya RNA (urugero RNAstable®) hanyuma ikoherezwa mubushyuhe bwicyumba.