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Urutonde rwa Chromatin Immunoprecipitation (ChIP-seq)
ChIP-Seq itanga genome-yerekana ishusho ya ADN yo guhindura amateka, ibintu byandikirwa, hamwe na poroteyine zijyanye na ADN.Ihuza uburyo bwo guhitamo chromatin immuno-imvura (ChIP) kugirango igarure poroteyine-ADN yihariye, hamwe nimbaraga zo gukurikiraho kuzakurikiraho (NGS) kugirango bikurikirane neza ADN yagaruwe.Byongeye kandi, kubera ko poroteyine-ADN igarurwa mu ngirabuzimafatizo, ibibanza bihuza bishobora kugereranywa mu bwoko butandukanye no mu ngingo zitandukanye, cyangwa mu bihe bitandukanye.Porogaramu iratandukanye kuva amabwiriza yo kwandikirana kugeza inzira yiterambere kugeza uburyo bwindwara ndetse nibindi.
Ihuriro: Illumina NovaSeq 6000
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Genome yose bisul fi te ikurikiranye
Methylation ya ADN kumwanya wa gatanu muri cytosine (5-mC) igira uruhare runini mumagambo ya gene no mubikorwa bya selile.Uburyo bwa methylation budasanzwe bwajyanye nibintu byinshi n'indwara nka kanseri.WGBS yahindutse zahabu yo kwiga genome-ubugari bwa methylation kumurongo umwe.
Ihuriro: Illumina NovaSeq6000
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Suzuma kuri Transposase-Yagerwaho Chromatin hamwe na Byinshi Byakurikiranye (ATAC-seq)
ATAC-seq nuburyo bukurikira bwo gutondekanya uburyo bwo gusesengura genome-yagutse ya chromatine, ifite akamaro kanini mugukwirakwiza epigenetike kwisi yose.Adapteri zikurikirana zinjizwa mukarere ka chromatin zifunguye na hyperactive Tn5 transposase.Nyuma ya PCR yongerewe imbaraga, hubatswe isomero rikurikirana.Uturere twose twa chromatin dufunguye turashobora kuboneka mugihe cyumwanya-wihariye, ntabwo bigarukira gusa kumwanya uhuza ibintu byandikirwa, cyangwa akarere runaka kahinduwe.
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Kugabanya Guhagararirwa Bisulfite Urukurikirane (RRBS)
Ubushakashatsi bwa methylation ya ADN buri gihe bwabaye ingingo ishyushye mubushakashatsi bwindwara, kandi bufitanye isano rya bugufi nimvugo ya gene hamwe na pheno-typic.RRBS nuburyo nyabwo, bukora neza kandi bwubukungu kubushakashatsi bwa methylation ya ADN.Gutunganya uturere twa CpG hamwe na CpG mukarere ka enzymatique (Msp I), hamwe na Bisulfite ikurikirana, bitanga ibisubizo bihanitse bya ADN methylation.
Ihuriro: Illumina NovaSeq 6000
