Urutonde rwa Metagenomic (NGS)
Inyungu za serivisi
ØKwigunga no guhinga-bidafite mikorobe
ØIcyemezo gihanitse mugutahura amoko make-mubidukikije
ØIgitekerezo cya "meta-" gihuza ibintu byose biologiya kurwego rwimikorere, urwego rwubwoko hamwe nurwego rwa gene, ibyo bikaba bigaragaza imbaraga zegeranye nukuri.
ØBMK ikusanya uburambe muburyo butandukanye bw'icyitegererezo hamwe na sample zirenga 10,000.
Ibisobanuro bya serivisi
| UrukurikiraneIhuriro | Isomero | Basabwe gutanga amakuru | Bigereranijwe guhindukira |
| Illumina NovaSeq 6000 | PE250 | 50K / 100K / 300K Tagi | Iminsi 30 |
Isesengura rya bioinformatics
üKugenzura ubuziranenge bwamakuru
üInteko ya Metagenome
üImirasire ya gene yashizweho na annotation
üUbwoko bwo gusesengura ibintu bitandukanye
üIsesengura ryimikorere itandukanye
üIsesengura hagati yitsinda
üIsesengura ryishyirahamwe rirwanya ibintu byubushakashatsi
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
KuriIbikuramo ADN:
| Ubwoko bw'icyitegererezo | Umubare | Kwibanda | Isuku |
| Ibikuramo ADN | ＞ 30 ng | ＞ 1 ng / μl | OD260 / 280 = 1.6-2.5 |
Kubidukikije:
| Ubwoko bw'icyitegererezo | Basabwe uburyo bwo gutoranya |
| Ubutaka | Umubare w'icyitegererezo: hafi.5 g;Ibintu bisigaye byumye bigomba gukurwa hejuru;Gusya ibice binini hanyuma unyure muri mm 2 muyungurura;Icyitegererezo cya Aliquot muri sterile EP-tube cyangwa cyrotube yo kubika. |
| Umwanda | Umubare w'icyitegererezo: hafi.5 g;Kusanya hamwe na aliquot ntangarugero muri sterile EP-tube cyangwa cryotube kugirango ubike. |
| Amara | Ingero zigomba gutunganywa mugihe cya aseptic.Koza imyenda yakusanyirijwe hamwe na PBS;Centrifuge PBS hanyuma ukusanyirize imvura muri EP-tubes. |
| Umuyoboro | Umubare w'icyitegererezo: hafi.5 g;Kusanya hamwe na aliquot sludge sample muri sterile EP-tube cyangwa cryotube kugirango ubike |
| Amazi | Kurugero hamwe na mikorobe nkeya, nkamazi ya robine, amazi meza, nibindi, Kusanya byibuze amazi 1 L hanyuma unyure muri 0.22 μm muyungurura kugirango ukungahaye mikorobe kuri membrane.Bika ururenda muri sterile. |
| Uruhu | Witonze witonze hejuru yuruhu ukoresheje ipamba ya sterile cyangwa urubingo rwo kubaga hanyuma ubishyire mubitereko bya sterile. |
Basabwe Gutanga Icyitegererezo
Hagarika icyitegererezo muri azote yuzuye mumasaha 3-4 hanyuma ubike muri azote yuzuye cyangwa -80 kugirango ubike igihe kirekire.Icyitegererezo cyoherezwa hamwe na ice-ice irakenewe.
Akazi ka serivisi
Icyitegererezo
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
1.Histogramu: Ubwoko bwo gukwirakwiza
2.Imikorere ikora isobanurwa kuri KEGG inzira ya metabolike
3.Gushushanya ikarita: Imikorere itandukanye ishingiye kubwinshi bwa gen
4.Circos ya CARD antibiotic irwanya genes
Urubanza rwa BMK
Ikwirakwizwa rya genoside irwanya antibiyotike hamwe na bagiteri zitera ku butaka-mangrove umuzi
Byatangajwe:Ikinyamakuru c'ibikoresho biteye akaga, 2021
Ingamba zikurikirana:
Ibikoresho: ADN ikuramo ibice bine byumuzi wa mangrove bifitanye isano: ubutaka butatewe, rhosikori, episphere hamwe nibice bya endosifera.
Ihuriro: Illumina HiSeq 2500
Intego: Metagenome
16S rRNA gene V3-V4 akarere
Ibisubizo by'ingenzi
Itondekanya rya metagenomic hamwe na metabarcoding ishushanya kubutaka-imizi ikomeza ingemwe za mangrove byakozwe kugirango bige ku ikwirakwizwa rya genoside irwanya antibiyotike (ARGs) ivuye mu butaka ikajya mu bimera.Imibare ya Metagenomic yerekanye ko 91.4% ya antibiyotike irwanya antibiyotike yakunze kugaragara mubice bine byose byavuzwe haruguru, byerekana imiterere ikomeza.16S rRNA ikurikirana ya amplicon yabyaye 29,285 ikurikiranye, igereranya amoko 346.Ufatanije nubwoko bugereranya na amplicon ikurikirana, uku gukwirakwiza wasangaga kutigenga kuri microbiota ifitanye isano n umuzi, ariko, birashobora koroherezwa na mobile ya element genetique.Ubu bushakashatsi bwerekanye imigendekere ya ARGs na virusi biva mu butaka mu bimera binyuze mu butaka-imizi ikomeza.
Reba
Wang, C., Hu, R., Mukomere, PJ, Zhuang, W., & Shu, L.(2020).Ikwirakwizwa rya genoside irwanya antibiyotike hamwe na bagiteri zitera ubutaka - mangrove root continuum.Ikinyamakuru c'ibikoresho biteye akaga, 408, 124985.
