Ubushinwa Metagenomic Ikurikirana-Uruganda rwa Nanopore n'abakora |BMK

Gukurikirana Metagenomic-Nanopore

Metagenomic Sequencing-Nanopore Featured Image

Metagenomics nigikoresho cya molekuline gikoreshwa mu gusesengura ibikoresho bivangwa na genomic bivanwa mu ngero z’ibidukikije, bitanga amakuru arambuye mu miterere y’ibinyabuzima no ku bwinshi, imiterere y’abaturage, isano ya phylogeneque, ingirabuzimafatizo ikora hamwe n’ibidukikije, n'ibindi. Kuri metagenomic.Imikorere yayo idasanzwe mubisomwa birebire byongerewe imbaraga zo gusesengura metagenomic, cyane cyane inteko ya metagenome.Ufashe ibyiza byo gusoma-uburebure, Nanopore ishingiye kuri metagenomic ubushakashatsi irashobora kugera ku nteko ikomeza ugereranije na metagenomics.Byashyizwe ahagaragara ko metagenomics ishingiye kuri Nanopore yabyaye genoside yuzuye kandi ifunze mikorobe (Moss, EL, et al.,Kamere ya Biotech, 2020)

Ihuriro ：Nanopore Isezerano P48

Twandikire

Ibisobanuro bya serivisi

Ibisubizo bya Demo

Urubanza rwa BMK

Inyungu za serivisi

ØIteraniro ryujuje ubuziranenge-Kuzamura ukuri kumenyekanisha amoko no guhanura gene

ØGufunga bagiteri gufunga

ØGukoresha imbaraga nyinshi kandi zizewe mubice bitandukanye, urugero nko kumenya mikorobe itera indwara cyangwa antibiyotike irwanya gen

ØKugereranya metagenome isesengura

Ibisobanuro bya serivisi

UrukurikiraneIhuriro	Isomero	Basabwe gutanga amakuru	Bigereranijwe guhindukira
Illumina NovaSeq 6000	PE250	50K / 100K / 300K Tagi	Iminsi 30

Isesengura rya bioinformatics

üKugenzura ubuziranenge bwamakuru

üInteko ya Metagenome

üImirasire ya gene yashizweho na annotation

üUbwoko bwo gusesengura ibintu bitandukanye

üIsesengura ryimikorere itandukanye

üIsesengura hagati yitsinda

üIsesengura ryishyirahamwe rirwanya ibintu byubushakashatsi

Icyitegererezo gisabwa no gutanga

Icyitegererezo gisabwa:

KuriIbikuramo ADN:

Ubwoko bw'icyitegererezo	Umubare	Kwibanda	Isuku
Ibikuramo ADN	＞ 30 ng	＞ 1 ng / μl	OD260 / 280 = 1.6-2.5

Kubidukikije:

Ubwoko bw'icyitegererezo	Basabwe uburyo bwo gutoranya
Ubutaka	Umubare w'icyitegererezo: hafi.5 g;Ibintu bisigaye byumye bigomba gukurwa hejuru;Gusya ibice binini hanyuma unyure muri mm 2 muyungurura;Icyitegererezo cya Aliquot muri sterile EP-tube cyangwa cyrotube yo kubika.
Umwanda	Umubare w'icyitegererezo: hafi.5 g;Kusanya hamwe na aliquot ntangarugero muri sterile EP-tube cyangwa cryotube kugirango ubike.
Amara	Ingero zigomba gutunganywa mugihe cya aseptic.Koza imyenda yakusanyirijwe hamwe na PBS;Centrifuge PBS hanyuma ukusanyirize imvura muri EP-tubes.
Umuyoboro	Umubare w'icyitegererezo: hafi.5 g;Kusanya hamwe na aliquot sludge sample muri sterile EP-tube cyangwa cryotube kugirango ubike
Amazi	Kurugero hamwe na mikorobe nkeya, nkamazi ya robine, amazi meza, nibindi, Kusanya byibuze amazi 1 L hanyuma unyure muri 0.22 μm muyungurura kugirango ukungahaye mikorobe kuri membrane.Bika ururenda muri sterile.
Uruhu	Witonze witonze hejuru yuruhu ukoresheje ipamba ya sterile cyangwa urubingo rwo kubaga hanyuma ubishyire mubitereko bya sterile.

Basabwe Gutanga Icyitegererezo

Hagarika icyitegererezo muri azote yuzuye mumasaha 3-4 hanyuma ubike muri azote yuzuye cyangwa -80 kugirango ubike igihe kirekire.Icyitegererezo cyoherezwa hamwe na ice-ice irakenewe.

Akazi ka serivisi

Icyitegererezo

Kubaka isomero

Urukurikirane

Isesengura ryamakuru

Serivisi nyuma yo kugurisha

Mbere: Genome yose bisul ﬁ te ikurikiranye

Ibikurikira: Urutonde rwa Metagenomic (NGS)

1.Ubushuhe: Ubwoko bukize2.Imikorere ikora isobanurwa kuri KEGG inzira ya metabolike3.Umuyoboro uhuza imiyoboro4.Circos ya CARD antibiotic irwanya genes

Urubanza rwa BMK

Nanopore metagenomics ituma isuzuma ryihuse ryindwara ya bagiteri yanduye

Byatangajwe:Kamere y’ibinyabuzima, 2019

Ibikurubikuru bya tekiniki
Urukurikirane: Nanopore MinION
Clinical metagenomics bioinformatics: Kwakira ADN igabanuka, WIMP na ARMA isesengura
Kumenya vuba: amasaha 6
Ubukangurambaga bukabije: 96,6%

Ibisubizo by'ingenzi

Mu mwaka wa 2006, kwandura indwara z'ubuhumekero (LR) byateje abantu miliyoni 3 ku isi.Uburyo busanzwe bwo kumenya indwara ya LR1 ni uguhinga, bufite sensibilité mbi, igihe kirekire kandi kikaba kidafite ubuyobozi mubuvuzi bwa antibiyotike hakiri kare.Isuzuma ryihuse kandi ryukuri rya mikorobe rimaze igihe gikenewe byihutirwa.Dr. Justin wo muri kaminuza y’iburasirazuba bwa Anglia na bagenzi be bakoze neza uburyo bwa Nanopore bushingiye kuri metagenomic yo kumenya indwara.Ukurikije akazi kabo, 99,99% ya ADN yakira irashobora kugabanuka.Kumenya virusi hamwe na antibiotique irwanya antibiyotike birashobora kurangira mumasaha 6.

Reba
Charalampous, T., Kay, GL, Richardson, H., Aydin, A., & O'Grady, J ..(2019).Nanopore metagenomics ituma isuzuma ryihuse ryindwara ya bagiteri yanduye.Kamere Ibinyabuzima, 37 (7), 1.