Ubushinwa Bacteria Zuzuye Uruganda ninganda |BMK

Indwara ya bagiteri yuzuye

Biomarker Technologies itanga serivise ikurikirana mugukora genome yuzuye ya bagiteri ifite icyuho cya zeru.Ibikorwa nyamukuru bya bagiteri byuzuye byubaka genome bikubiyemo ibisekuruza bya gatatu bikurikirana, guteranya, gutangaza imikorere hamwe nisesengura rya bioinformatic ryujuje intego zubushakashatsi.Ibisobanuro birambuye kuri genoside ya bagiteri itanga imbaraga zerekana uburyo bwibanze bushingiye ku binyabuzima, ibyo bikaba byanatanga ibisobanuro bifatika kubushakashatsi bwa genomique mu bwoko bwa eukaryotic.

Ihuriro ：Nanopore Isezerano P48 + Illumina NovaSeq 6000

Urutonde rwa PacBio II

Twandikire

Ibisobanuro bya serivisi

Ibisubizo bya Demo

Inyungu za serivisi

ØIngamba nyinshi zikurikirana ziboneka kumigambi itandukanye yubushakashatsi

ØIndwara ya bagiteri yuzuye genome hamwe na 0 Gap yemewe.

ØInararibonye cyane muri bacteri genome ikorana na genome zirenga 10,000.

ØUmwuga nyuma yo kugurisha itsinda ryunganira tekinike ryujuje ibyifuzo byubushakashatsi bwihariye.

Ibisobanuro bya serivisi

UrukurikiraneIngamba

Isomero

Ubwiza bufite ireme

Bigereranijwe guhindukira

Nanopore 100X + Illumina 50X

Nanopore 20K

PE150

0 Icyuho

Iminsi 30

PacBio HiFi 30X

PacBio 10K

Isesengura rya bioinformatics

üKugenzura ubuziranenge bwamakuru

üInteko rusange

üIsesengura ryibigize genome

üImikorere ya gen

üKugereranya genomic igereranya

Icyitegererezo gisabwa no gutanga

Icyitegererezo gisabwa:

KuriIbikuramo ADN:

Ubwoko bw'icyitegererezo	Umubare	Kwibanda	Isuku
Ibikuramo ADN	Μ 3 μg	Ng 20 ng / μl	OD260 / 280 = 1.6-2.5

Ku byitegererezo by'inyama:

Ubwoko bw'icyitegererezo

Basabwe kuvura icyitegererezo

Icyitegererezo cyo kubika no kohereza

Indwara ya bagiteri

Itegereze bagiteri munsi ya microscope hanyuma ukusanye za bagiteri mugice cyazo

Hindura umuco wa bagiteri (urimo selile zigera kuri 3-4.5e9) muri eppendorf ya 1.5 cyangwa 2.(Komeza ku rubura)

Centrifuge umuyoboro muminota 1 kuri 14000 g kugirango ukusanye bagiteri kandi ukureho supernatant witonze

Funga umuyoboro hanyuma uhagarike bagiteri muri azote yuzuye byibuze 30 min.Bika umuyoboro muri -80 frigo.

Hagarika icyitegererezo muri azote yuzuye mumasaha 3-4 hanyuma ubike muri azote yuzuye cyangwa -80 kugirango ubike igihe kirekire.Icyitegererezo cyoherezwa hamwe na ice-ice irakenewe.