UBUKUNGU BWA MICROBIAL
Kwiyongera k'ubuhinzi bigabanya urusobe rwa mikorobe kandi ubwinshi bwa tagisi yingenzi mumuzi
Uburebure bwuzuye bwa Amplicon (ITS) |PacBio HIFI
Ingingo z'ingenzi
Akongera ingufu mu buhinzi byarushijeho kuba ikibazo bitewe n’ingaruka mbi z’ibidukikije zirimo gukoresha nabi intungamubiri, gukoresha amazi yo mu butaka, kwangirika kw’ubutaka, n'ibindi.Umuryango wa mikorobe ugira uruhare rukomeye mumusaruro no kuramba kwa agroecosystem.Ariko, ntibisobanutse neza ko uburyo butandukanye bwo guhinga bugira ingaruka kuri microbiota.
Igishushanyo mbonera
Ubushakashatsi
Sicyitegererezo cyamavuta numuzi (ADN) byaturutse kumirima yingano kuva mubuhinzi 60 (20 buri)
Ggutondeka: 1. Amasezerano (hamwe no guhinga);2. Amasezerano (nta-guhinga);3. Ubuhinzi-mwimerere
Singamba zo kuringaniza: Urutonde rwuzuye rwa amplicon (ITS)
Primers: ITS1F-ITS4 (ireba akarere kose ka ITS ~ 630 bp)
Skuringaniza urubuga: PacBio RS II
Isesengura ryibinyabuzima
Ibisubizo
Oimpuzandengo ya 357 OTU yamenyekanye kurubuga hamwe na 837 OTU zose kurubuga 60.Alpha itandukanye yimizi yibihumyo ntabwo yerekanaga itandukaniro rinini muburyo butatu bwo guhinga.Nyamara, amatsinda atatu atandukanye yashizweho mubisesengura rya beta, byerekana ingaruka zikomeye za sisitemu yo guhinga kumiterere yumudugudu.
Igicapo 1. Ubudasa bwa Alpha (Indangantego ya Shannon hamwe nabagize umuryango) hamwe nisesengura rya beta itandukanye (isesengura rya Canonical of coordinateurs) kumiryango yibihumyo.
Ten tagisi yingenzi yasobanuwe hashingiwe kumurongo rusange wimiryango yibihumyo muri sisitemu eshatu zubuhinzi: imitwe 10 yambere ifite impamyabumenyi ihanitse, ihuriro ryegereye cyane hamwe hagati yo hagati.Birindwi muri byo byari ibyateganijwe na mycorrhizal.
Igishushanyo 2. Muri rusange urusobe kumiryango yibihumyo ya sisitemu eshatu zo guhinga
Fintwaro-sisitemu yihariye yerekana imiyoboro ihanitse cyane murusobe kama hamwe ninshuro ebyiri zose hamwe nu murongo uhujwe kuruta no-kugeza hamwe numuyoboro usanzwe.Byongeye kandi, urusobe rw’ubuhinzi-mwimerere rwarimo tagisi yingenzi (diyama) ugereranije nizindi, zashyigikiraga ubunini bwazo no guhuza
Igicapo 3. Sisitemu yo guhinga yihariye imiyoboro yumuzi
AIhuriro rikomeye hagati yubuhinzi nimbaraga zihuza imiyoboro yabonetse.Isesengura ry’amashyamba risanzwe ryagaragaje abashoferi nyamukuru ba tagisi yingenzi: fosifore yubutaka, ubwinshi bwinshi, pH hamwe na koloni ya mycorrhizal.
Igicapo 4. Ubwinshi bwubuhinzi no guhuza imiyoboro muburyo butatu bwo guhinga (A na B);Isesengura ryamashyamba risanzwe (C) nubusabane hagati yubuhinzi nubukoloni bwa AMF (D)
Ikoranabuhanga
Urutonde rwuzuye rwa amplicon
As "Urutonde rwibisekuru bya gatatu" biza kuri stage, imbogamizi mukarere kagenewe hamwe nibibazo byo guterana kwa novo byatsinzwe.Pasifika Bioscience (PacBio) yaguye neza gusoma bikurikirana kugeza kuri kilobase icumi, bidufasha kubona ibisobanuro byuzuye bya 16s rRNA (1.000 bp-1.500 bp) muri bagiteri cyangwa 18S rRNA (1.500 bp-2000 bp) na ITS uturere (400 bp-900 bp) muri eukaryotics.Kugura kwagutse kumurima wa genetike byongereye cyane gukemura amoko yo gutangaza amoko hamwe na gen ikora.Ikibazo kirekire gihangayikishije ishingiro ryukuri cyakemuwe na PacBio CCS kwikosora, itanga HIFI gusoma hamwe na 99% byasomwe neza.
Imikorere muri annotation ya OTU
Taking ibyiza byombi bisomwa birebire kandi byinjira cyane, ibisobanuro bya annotasiyo birashobora kwiyongera kuburyo bugaragara no kugera kumurongo wubwoko "murwego rwo kumenya mikorobe."
Reba
Banerjee, Samiran, n'abandi.“Kwiyongera k'ubuhinzi bigabanya urusobe rwa mikorobe ndetse n'ubwinshi bwa tagisi y'amabuye mu mizi.”Ikinyamakuru ISME (2019).
Ikoranabuhanga hamwe nibyingenzi igamije gusangira ibyagezweho vuba muburyo butandukanye bwo gukoresha tekinoroji ikurikirana muburyo butandukanye hamwe nibitekerezo byiza mugushushanya no gucukura amakuru.
Igihe cyo kohereza: Mutarama-08-2022