MICROBIAL ECOLOGY
Ƙarfafa aikin noma yana rage rikitacciyar hanyar sadarwa ta ƙananan ƙwayoyin cuta da kuma yawan harajin maɓalli a cikin tushen
Tsarin Amplicon mai cikakken tsayi (ITS) |Farashin HIFI
Karin bayanai
AƘarfafa aikin noma ya ƙara samun matsala saboda munanan illolinsa na muhalli da suka haɗa da rashin ingantaccen amfani da abinci mai gina jiki, kawar da ruwan goundt, lalata ingancin ƙasa, da dai sauransu. Sauran tsarin noman da suka haɗa da noman noma da na halitta an yi amfani da su sosai don rage cutar.Ƙungiyoyin ƙananan ƙwayoyin cuta suna taka muhimmiyar rawa a cikin yawan aiki da dorewar tsarin noma.Koyaya, ba a san yadda tsarin noma daban-daban ke shafar tushen microbiota ba.
Tsarin Gwaji
Gwaje-gwaje
Ssamfurorin mai da tushen (DNA) sun fito ne daga filayen alkama daga filayen noma 60 (kowace 20)
Grouping: 1. Yarjejeniya (tare da noma);2. Yarjejeniya (ba nono);3. Filayen noma na halitta
SDabarun equencing: Cikakken-tsawon amplicon sequencing (ITS)
PRimers: ITS1F-ITS4 (yana nufin duk yankin ITS ~ 630 bp)
Sequencing dandamali: PacBio RS II
Bioinformatic Analysis
Sakamako
On matsakaita na 357 OTU an gano su a kowane wuri kuma jimillar OTU 837 na duk shafuka 60.Bambance-bambancen Alpha na tushen fungi na al'ummomin ba su nuna babban bambanci tsakanin tsarin noma guda uku ba.Koyaya, an ƙirƙiri gungu daban-daban guda uku a cikin binciken bambance-bambancen beta, suna nuna tasirin tsarin noma akan tushen tsarin al'umma na fungal.
Hoto 1. Bambancin Alpha (Shannon index da al'umma abun da ke ciki) da kuma nazarin bambancin beta (binciken canonical na manyan haɗin gwiwar) akan tushen al'ummomin fungal.
Ten keystone haraji an bayyana bisa ga cikakken cibiyar sadarwa na fungal al'ummomin fadin tsarin noma uku: saman 10 nodes tare da mafi girma mataki, mafi kusancin tsakiya da mafi ƙasƙanci tsakani aka zaba.Bakwai daga cikinsu na cikin odar mycorrhizal ne.
Hoto 2. Gabaɗaya cibiyar sadarwa akan tushen fungal al'ummomin tsarin noma guda uku
FƘayyadaddun hanyoyin sadarwa-tsarin makamai sun nuna babban haɗin kai a cikin hanyar sadarwa ta halitta tare da ƙarin gefuna sau biyu da ƙarin haɗin haɗin gwiwa fiye da babu-har sai cibiyar sadarwa ta al'ada.Bugu da ƙari, cibiyar sadarwar noma ta kwayoyin halitta ta ƙunshi ƙarin haraji mai mahimmanci (lu'u-lu'u) idan aka kwatanta da sauran, wanda ke goyan bayan rikitarwa da haɗin kai.
Hoto 3. Tsarin noma-takamaiman tushen hanyoyin sadarwa na fungal
Aƙaƙƙarfan ƙaƙƙarfan haɗin kai tsakanin ƙarfin aikin gona da tushen haɗin gwiwar cibiyar sadarwa na fungal an lura.Binciken gandun daji bazuwar ya bayyana manyan abubuwan da ke haifar da taxa keystone: ƙasa phosphorus, yawa mai yawa, pH da mycorrhizal colonization.
Hoto 4. Ƙarfin aikin noma da haɗin yanar gizo a cikin tsarin noma guda uku (A da B);Binciken gandun daji na bazuwar (C) da alaƙa tsakanin ƙarfin aikin gona da mulkin mallaka na AMF (D)
Fasaha
Tsarin amplicon cikakken tsayi
As "Sequencing Generation Uku" yana zuwa kan mataki, an shawo kan iyakokin yankunan da aka yi niyya da matsaloli a taron de novo.Pacific Bioscience (PacBio) ya sami nasarar tsawaita karatun jeri zuwa dubun kilobases, wanda ke ba mu damar samun cikakken karatun 16s rRNA (1,000 bp-1,500 bp) a cikin kwayoyin cuta ko 18S rRNA (1,500 bp-2,000 bp) da ITS yankuna (400 bp-900 bp) a cikin eukaryotics.Faɗin ra'ayi game da filin kwayoyin halitta ya inganta ƙudirin bayanin nau'in nau'in halitta da kwayoyin aiki.PacBio CCS gyara kansa ya warware batun dogon lokaci da aka damu akan daidaiton tushe, wanda ke haifar da karatun HIFI tare da daidaiton karantawa sama da 99%.
Ayyuka a cikin bayanin OTU
TAking fa'idodin duka dogon karantawa da babban aiki, daidaiton bayanin ana iya ƙara girma da kuma cimma ƙudurin "matakin nau'in" a cikin gano ƙananan ƙwayoyin cuta.
Magana
Banerjee, Samiran, et al."Ƙarfafa aikin noma yana rage sarƙaƙƙiyar hanyar sadarwa ta microbial da kuma yawan harajin maɓalli a cikin tushen."Jaridar ISME (2019).
Fasaha da Manyan Labarai yana da nufin raba mafi kyawun aikace-aikacen nasara na baya-bayan nan na fasahohi daban-daban na babban abin da ake aiwatarwa a fagen bincike daban-daban da kuma ƙwararrun dabaru a ƙirar gwaji da hakar bayanai.
Lokacin aikawa: Janairu-08-2022