MICROBIAL ECOLOGY
Kuwedzerwa kwekurima kunoderedza microbial network kuoma uye kuwanda kwekeystone taxa mumidzi.
Yakazara-kureba Amplicon Sequencing (ITS) |PacBio HIFI
Pfungwa huru
Akusimba kwekurima kwave kuchiwedzera kunetsa nekuda kwekukanganisa kwaro kwezvakatipoteredza kunosanganisira kusashanda zvakanaka kwekudya kwekudya, goundwater eutrophiation, kuderedzwa kwemhando yevhu, etc. Mamwe maitiro ekurima anosanganisira kwete-till uye organic farming ave akagamuchirwa zvakanyanya kuderedza kukuvadza.Nharaunda yeMicrobial inoita basa rinokosha mukubereka uye kuenderera mberi kweagroecosystem.Nekudaro, hazvina kujeka kuti nzira dzakasiyana dzekurima dzinokanganisa sei midzi microbiota.
Kuedza Dhizaini
Miedzo
Soiri nemidzi (DNA) samples dzaibva kuminda yegorosi kubva ku60 minda yekurima (20 imwe neimwe)
Gkudzura: 1. Kokorodzano (nekurima);2. Kokorodzano (pasina-kurima);3. Organic farmland
Sequencing zano: Yakazara-kureba amplicon sequencing (ITS)
Primers: ITS1F-ITS4 (yakananga iyo yese ITS dunhu ~630 bp)
Sequencing chikuva: PacBio RS II
Bioinformamatic Analysis
Results
On avhareji ye357 OTUs yakaonekwa pane imwe saiti uye yakazara ye837 OTU yenzvimbo dzese makumi matanhatu.Alpha siyana yemidzi fungal nharaunda yanga isingaratidzi musiyano wakakosha pakati pehutatu hwekurima system.Nekudaro, masumbu matatu akasiyana akaumbwa mukuongororwa kwemhando yebeta, zvichiratidza mhedzisiro yakasimba yehurongwa hwekurima pamidzi fungal nharaunda chimiro.
Mufananidzo 1. Alpha siyana (Shannon indekisi uye kuumbwa kwenharaunda) uye beta siyana ongororo (canonical ongororo yeanonyanya kuronga) pamidzi fungal nharaunda.
Ten keystone taxa yakatsanangurwa zvichibva pane yakazara network yefungal nharaunda munzvimbo nhatu dzekurima: epamusoro 10 node ane dhigirii repamusoro, hukama hwepedyo hwepakati uye hwakaderera pakati pepakati hwakasarudzwa.Zvinomwe zvacho zvaiva zvemirairo yemycorrhizal.
Mufananidzo 2. Mambure akazara pamidzi fungal nharaunda dzemhando nhatu dzekurima
Farming-system chaiyo network yakaratidza kubatana kwakakwira zvakanyanya mu organic network ine kaviri mipendero uye akawanda akabatana node pane kwete-till uye yakajairika network.Zvakare, iyo organic farming network yakabata yakawanda keystone taxa (dhaimondi) zvichienzaniswa nemamwe, ayo aitsigira kuoma kwayo uye kubatana.
Mufananidzo 3. Kurima system-yakananga midzi fungal network
Akushamwaridzana kwakasimba kwakashata pakati pekusimba kwekurima uye midzi fungal network yekubatanidza yakaonekwa.Ongororo yesango isingaite yakaratidza madhiraivha makuru emutero wematombo makuru: ivhu phosphorus, kuwanda kwehuwandu, pH uye mycorrhizal colonization.
Mufananidzo 4. Kusimba kwekurima uye kubatana kwetiweki mumasisitimu matatu ekurima (A neB);Random sango kuongororwa (C) uye Hukama pakati pekurima zvakanyanya uye AMF colonization (D)
Vadivelu Comedy Technology
Yakazara-kureba amplicon sequencing
As "Yechitatu Yechizvarwa Sequencing" ichiuya pachikuva, izvo zvisingakwanisi munzvimbo dzakanangwa uye matambudziko mumusangano we de novo akakundwa.Pacific Bioscience (PacBio) yakabudirira kuwedzera kuverenga kwekutevedzana kusvika kumakumi emakiromita, izvo zvinotibvumira kuwana kuverenga kwakazara kwe16s rRNA (1,000 bp-1,500 bp) mubhakitiriya kana 18S rRNA (1,500 bp-2,000 bp) uye ITS matunhu (400 bp-900 bp) mu eukaryotic.Maonero akatambanudzwa eiyo genetic field yakawedzera zvakanyanya kugadziriswa kwezvipenyu zvinotsanangurwa uye anoshanda majini.Iyo yakareba yakabatikana nyaya pamusoro pekurongeka kwepasi yakagadziriswa nePacBio CCS yekuzvigadzirisa, iyo inoburitsa HIFI inoverengwa nepamusoro pe99% kuverenga chaiko.
Kuita muOTU annotation
Tuchitsvaga zvakanakira zvese zviri zviviri kuverenga kwenguva refu uye yakakwirira-kuburikidza, iko kurongeka kweanotidziro kunogona kuwedzera zvinoshamisa uye kuwana "mhando-yemhando" sarudzo mukuzivikanwa kwemicrobial.
Reference
Banerjee, Samiran, et al."Kuwedzera kwekurima kunoderedza kuomarara kwehutachiona uye kuwanda kwemutero wedombo mumidzi."Iyo ISME Journal (2019).
Tech uye Highlights inovavarira kugovera zvichangobva kuitika zvemashandisirwo akasiyana-siyana epamusoro-soro ekutevedzanisa matekinoroji munzvimbo dzakasiyana siyana dzekutsvagisa pamwe chete nemazano akajeka mukugadzira dhizaini nekuchera data.
Nguva yekutumira: Jan-08-2022