IMICROBIAL Ekholoji
Ukuqina kwezolimo kunciphisa ubunzima benethiwekhi ye-microbial kunye nobuninzi be-tax yelitye elingundoqo kwiingcambu
Ukulandelelana kweAmplicon yobude obupheleleyo (ITS) |PacBio HIFI
Iimbalasane
AUgxininiso lwezolimo luye lwaba yingxaki ngenxa yeziphumo zalo ezibi zokusingqongileyo ezibandakanya ukungasetyenziswanga kakuhle kwezondlo, i-goutrophiation yamanzi aphantsi komhlaba, ukuthotywa komgangatho womhlaba, njl.njl. Iindlela ezizezinye zokulima eziquka ukungaphethuli umhlaba kunye nokulima ngendalo ziye zamkelwa ngokubanzi ukunciphisa umonakalo.Uluntu lweMicrobial ludlala indima ebaluleke kakhulu kwimveliso nozinzo lwenkqubo yezolimo.Nangona kunjalo, ayicacanga into yokuba iindlela ezahlukeneyo zokufama zichaphazela iingcambu zemicrobiota.
Uyilo loMfuniselo
Iimvavanyo
SIoyile kunye neengcambu (i-DNA) iisampulu zazisuka kumasimi engqolowa asuka kuma-60 eefama zokulima (ama-20 inye)
Gukuronta: 1. Indibano (ngokulima);2. Indibano (akumhlaba);3. Umhlaba weefama eziphilayo
Siqhinga lokulinganisa: Ukulandelelana kweamplicon yobude obupheleleyo (ITS)
Piirimers: ITS1F-ITS4 (ijolise kuwo wonke ummandla ITS ~630 bp)
Siqonga equencing: PacBio RS II
Uhlalutyo lwe-Bioinformatic
Iziphumo
Oumyinge wama-357 OTUs achongiwe kwindawo nganye kunye netotali yama-837 OTU kuzo zonke iziza ezingama-60.Ukwahlukana kwe-alpha kweengcambu zoluntu lomngundo bekungabonakali mahluko ubalulekileyo phakathi kwenkqubo yokulima ezintathu.Nangona kunjalo, amaqela amathathu awohlukileyo aye asekwa kuhlalutyo lokwahlukana kwe-beta, ebonisa iziphumo ezinamandla zenkqubo yokulima kubume beengcambu zomngundo woluntu.
Umzobo 1. Ukwahluka kwe-alpha (isalathisi se-Shannon kunye nokwakhiwa koluntu) kunye nohlalutyo lokwahluka kwe-beta (uhlalutyo lwe-canonical lolungelelwaniso oluyintloko) kwiingcambu zoluntu lomngundo.
Ten i-keystone taxa yachazwa ngokusekwe kuthungelwano lulonke loluntu olungumngundo kwiinkqubo ezintathu zokufama: iindawo eziphambili ezili-10 ezineqondo eliphezulu, ukusondelelana okuphezulu okusembindini kunye nokuphakathi kokuphakathi okuphantsi kuye kwakhethwa.Ezisixhenxe kuzo zazizezomyalelo we-mycorrhizal.
Umzobo 2. Uthungelwano lulonke kwiingcambu zomngundo kwiinkqubo ezintathu zokufama
Futhungelwano oluthile lwenkqubo yokuxhobisa lubonise uqhagamshelo oluphezulu kakhulu kuthungelwano lwendalo olunemiphetho ephindwe kabini kunye neendawo eziqhagamshelwe ngaphezulu kunokungaphethuli kunye nenethiwekhi eqhelekileyo.Ngaphaya koko, uthungelwano lwezolimo oluphilayo lunexabiso elithe kratya letaxa (diamond) xa lithelekiswa nezinye, ezixhasa ukuntsonkotha kwayo kunye noqhagamshelo.
Umfanekiso 3. Uthungelwano lweengcambu zokungunda olungqale kwinkqubo yokulima
AUnxulumano olomeleleyo olubi phakathi koxinzelelo lwezolimo kunye noqhagamshelo lothungelwano lomngundo lweengcambu lwabonwa.Uhlalutyo lwamahlathi olungacwangciswanga lubonise abaqhubi abaphambili berhafu yelitye elingundoqo: i-phosphorus yomhlaba, ukuxinana kwesambuku, i-pH kunye ne-mycorrhizal colonization.
Umfanekiso 4. Ukuqina kwezolimo kunye noqhagamshelwano lothungelwano kwiinkqubo ezintathu zokufama (A no-B);Uhlalutyo lwehlathi olungenamkhethe(C) kunye nobudlelwane phakathi kokuqina kwezolimo kunye ne-AMF colonization (D)
Iteknoloji
Ukulandelelana kwe-amplicon yobude obupheleleyo
As "Ulandelelwano lweSizukulwana sesiThathu" esiza eqongeni, imida kwimimandla ekujoliswe kuyo kunye neengxaki kwindibano ye-de novo yoyisiwe.I-Pacific Bioscience (i-PacBio) iye yandisa ngempumelelo ukufundwa kokulandelelana ukuya kumashumi eekilobase, okusivumela ukuba sifumane ubude obupheleleyo be-16s rRNA (1,000 bp-1,500 bp) kwiibhaktheriya okanye i-18S rRNA (1,500 bp-2,000 bp) kunye ne-ITS imimandla (400 bp-900 bp) kwi-eukaryotic.Imbono ebanzi yendawo yemfuza yaphucula kakhulu ukusonjululwa kwenkcazo yeentlobo kunye nofuzo olusebenzayo.Umcimbi omde ochaphazelekayo malunga nokuchaneka kwesiseko uye wasonjululwa yi-PacBio CCS yokuzilungisa, eyenza i-HIFI ifundeke ngokuchaneka kokufunda okungaphezulu kwe-99%.
Ukusebenza kwinkcazo ye-OTU
Taking iingenelo zombini ukufundwa okude kunye ne-high-throughput, ukuchaneka kwe-annotation kunokunyuswa ngokumangalisayo kunye nokufezekisa isisombululo "sezinga leentlobo" ekuchongeni i-microbial.
Isalathiso
Banerjee, Samiran, et al."Ukuqiniswa kwezolimo kunciphisa ubunzima bothungelwano lwe-microbial kunye nobuninzi berhafu engundoqo kwiingcambu."Ijenali ye-ISME (2019).
ITekhnoloji kunye neeNgcaciso ijolise ekwabelaneni ngeyona ndlela iphumeleleyo yamvanje yosetyenziso lweetekhnoloji zolandelelwano oluphezulu kwiindawo ezahlukeneyo zophando kunye neengcinga ezikrelekrele kuyilo lovavanyo kunye nokwembiwa kwedatha.
Ixesha lokuposa: Jan-08-2022