Eukaryotic mRNA Sequencing-Illumina

mRNA sequencing inogonesa profiling yeese mRNAs akanyorwa kubva kumaseru pasi pemamiriro chaiwo.Iyo tekinoroji ine simba yekuburitsa chimiro chejeni, zvimiro zvemajini uye mamorekuru emamwe maitiro ebhayoloji.Parizvino, kutevedzana kwemRNA kwave kushandiswa zvakanyanya mukutsvaga kwakakosha, kuongororwa kwekiriniki, kukura kwezvinodhaka, nezvimwe.

Platform: Illumina NovaSeq 6000

Taura nesu

Service Details

Bioinformatics

Demo Results

Zvakanakira

● Zvakanyanya Kuitika: Pamusoro pe200,000 samples dzakagadziriswa muBMK inovhara marudzi akasiyana-siyana emuenzaniso, kusanganisira sero tsika, tishu, mvura yemuviri, nezvimwewo uye pamusoro pe7,000 mRNA-Seq mapurojekiti akavharwa achivhara nzvimbo dzakasiyana-siyana dzekutsvakurudza.

● Strict quality control system: Core quality control points kuburikidza nematanho ese anosanganisira kugadzirira sampuli, kugadzirira raibhurari, sequencing uye bioinformatics zviri pasi pekutariswa kwepedyo kuitira kuti vape mhedzisiro yemhando yepamusoro.

● Madhatabheti akawanda anowanikwa echiziviso chebasa uye zvidzidzo zvekusimudzira kuzadzisa zvinangwa zvetsvagiridzo zvakasiyana.

● Mushure mekutengesa masevhisi: Mushure mekutengesa masevhisi anoshanda kwemwedzi mitatu pakupedzwa kweprojekiti, kusanganisira kutevera mapurojekiti, kupfura-dambudziko, mhinduro Q&A, nezvimwe.

Sample Zvinodiwa uye Delivery

Library	Sequencing strategy	Data inokurudzirwa	Quality Control
Poly A yakafumiswa	Illumina PE150	6 Gb	Q30≥85%

Sample Zvinodiwa:

Nucleotides:

Conc.(ng/μl)

Mari (μg)

Kuchena

Kuperera

≥ 20

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Yakaganhurirwa kana isina mapuroteni kana kusvibiswa kweDNA kunoratidzwa pagel.

Kumiti: RIN≥6.5;

Zvemhuka: RIN≥7.0;

5.0≥28S/18S≥1.0;

kukwirira kwakadzika kana kusina kwekutanga

Tissue: Huremu (yakaoma):≥1 g
*Kune tishu diki pane 5 mg, tinokurudzira kutumira flash yakaomeswa nechando (mumvura nitrogen) sampuli yetishu.

Kumiswa kwesero:Nhamba yemasero = 3 × 10⁶1 × 10⁷
* Isu tinokurudzira kutumira chando cell lysate.Kana iyo sero inoverenga idiki pane 5 × 105.

Mienzaniso yeropa:Vhoriyamu≥1 ml

Microorganism:Husa ≥ 1 g

Yakakurudzirwa Sample Delivery

Container: 2 ml centrifuge chubhu (Tin foil haina kukurudzirwa)

Sample labeling: Boka+ replicate eg A1, A2, A3;B1, B2, B3...

Shipment:

Dry-ice: Samples dzinofanirwa kutakurwa mumabhegi uye kuvigwa mune yakaoma-aizi.
RNAstable chubhu: RNA samples inogona kuomeswa muRNA kudzikamisa chubhu (eg RNAstable®) uye kutumirwa mukamuri tembiricha.