China Eukaryotic mRNA sequencing-Illumina fekitori uye vagadziri |BMK

Eukaryotic mRNA sequencing-Illumina

mRNA sequencing inogonesa profiling yeese mRNAs akanyorwa kubva kumaseru pasi pemamiriro chaiwo.Iyo tekinoroji ine simba yekuburitsa chimiro chejeni, zvimiro zvemajini uye mamorekuru emamwe maitiro ebhayoloji.Parizvino, kutevedzana kwemRNA kwave kushandiswa zvakanyanya mukutsvaga kwakakosha, kuongororwa kwekiriniki, kukura kwezvinodhaka, nezvimwe.

Platform: Illumina NovaSeq 6000

Taura nesu

Service Details

Bioinformatics

Demo Results

Zvakanakira

ØZvakanyanya Kuitika: Pamusoro pe200,000 samples dzakagadziriswa muBMK ichivhara akasiyana emhando dzemuenzaniso, kusanganisira sero tsika, tishu, mvura yemuviri, nezvimwewo uye anopfuura zviuru zvinomwe zveMRNA-Seq mapurojekiti akavharwa achivhara nzvimbo dzakasiyana dzekutsvagisa.

ØStrict quality control system: Core quality control points kuburikidza nematanho ese anosanganisira kugadzirira sampuli, kugadzirira raibhurari, kutevedzana uye bioinformatics zviri pasi pekutariswa kwepedyo kuitira kuti vape mhedzisiro yemhando yepamusoro.

ØAkawanda dhatabhesi anowanikwa ekuita zvirevo uye zvidzidzo zvekusimudzira kuzadzisa zvinangwa zvetsvagiridzo zvakasiyana.

ØMushure mekutengesa masevhisi: Mushure mekutengesa masevhisi anoshanda kwemwedzi mitatu pakupedzwa kweprojekiti, kusanganisira kutevera mapurojekiti, kupfura-dambudziko, mhinduro Q&A, nezvimwe.

Sample Zvinodiwa uye Delivery

Library	Sequencing strategy	Data inokurudzirwa	Quality Control
Poly A yakafumiswa	Illumina PE150	6 Gb	Q30≥85%

Sample Zvinodiwa:

Nucleotides:

Kuchena	Kuperera	Mari
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Yakaganhurirwa kana isina mapuroteni kana kusvibiswa kweDNA kunoratidzwa pagel.	Zvezvirimwa: RIN≥6.5;Zvemhuka: RIN≥7;28S/18S≥1.0;inogumira kana isina kukwirira kwekutanga	Conc.≥30 ng/μl;Vhoriyamu ≥ 10 μl;Zvose ≥ 1.5 μg

Tissue: Huremu (yakaoma):≥1 g
*Kune tishu diki pane 5 mg, tinokurudzira kutumira flash yakaomeswa nechando (mumvura nitrogen) sampuli yetishu.

Kumiswa kwesero:Nhamba yemasero = 3 × 10⁶1 × 10⁷
* Isu tinokurudzira kutumira chando cell lysate.Kana iyo sero ichiverengera idiki pane 5 × 105, flash yakaomeswa mune yemvura nitrogen inokurudzirwa, iyo inosarudzika kudhirowa diki.

Mienzaniso yeropa:Vhoriyamu≥1 ml

Microorganism:Husa ≥ 1 g

Yakakurudzirwa Sample Delivery

Container: 2 ml centrifuge chubhu (Tin foil haina kukurudzirwa)

Sample labeling: Boka+ replicate eg A1, A2, A3;B1, B2, B3...

Shipment:

Dry-ice: Samples dzinofanirwa kutakurwa mumabhegi uye kuvigwa mune yakaoma-aizi.
RNAstable chubhu: RNA samples inogona kuomeswa muRNA kudzikamisa chubhu (eg RNAstable®) uye kutumirwa mukamuri tembiricha.