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Genome-wide Association Analysis
Genome-wide association study (GWAS) ine chinangwa chekutsvaga mazita akasiyana-siyana (genotype) ane hukama nemaitiro chaiwo (phenotype).Chidzidzo cheGWAS chinoongorora ma genetic markers anoyambuka genome yenhamba huru yevanhu uye inofanotaura genotype-phenotype masangano kuburikidza nenhamba yekuongorora pahuwandu hwevanhu.Yakashandiswa zvakanyanya mukutsvagisa zvirwere zvevanhu uye kushanda kwemajini kumigodhi pahunhu hwakaoma hwemhuka kana zvirimwa.
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Chirimwa/Mhuka Yese Genome Sequencing
Yese genome re-sequencing, inozivikanwawo seWGS, inogonesa kuburitswa kwezvose zvakajairika uye zvisingawanzo shanduko pajenome rese kusanganisira Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), uye Copy Number Variation (CNV). )SVs inogadzira chikamu chikuru chekusiyana kwekusiyana kweSNPs uye ine simba guru pane genome, iyo ine simba guru pane zvipenyu.Kuverengera-kurebesa requencing kunobvumira kunyatsozivikanwa kwezvimedu zvakakura uye misiyano yakaoma nekuti kuverenga kwenguva refu kunoita kuti zvive nyore kuyambuka chromosomal kuyambuka matunhu akaoma senge tandem kudzokorora, GC / AT-akapfuma matunhu, uye hyper-anosiyana matunhu.
Platform: Illumina, PacBio, Nanopore
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Evolutionary Genetics
Evolutionary genetics ibasa rakazara rekutevedzana rakagadzirirwa kupa dudziro yakazara paruzivo rwekushanduka kwezvinhu zvakapihwa zvichibva mukusiyana kwemajini, kusanganisira SNPs, InDels, SVs uye CNVs.Inopa kuongorora kwese kwakakosha kunodiwa pakutsanangura shanduko yekushanduka-shanduka uye maumbirwo emarudzi evanhu, zvakadai sehuwandu hwevanhu, kusiyana kwemajini, hukama hwefilogeny, nezvimwewo. Iinewo zvidzidzo pamusoro pekuyerera kwemajini, izvo zvinopa simba kufungidzira kwehuwandu hwevanhu hunobudirira, nguva yekusiyana.
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Kuenzanisa Genomics
Comparative genomics zvinoreva kuenzanisa kutevedzana kwegenome yakazara uye zvimiro zvemarudzi akasiyana.Chirango ichi chine chinangwa chekuburitsa kushanduka kwemhando, basa remajini, nzira yekudzora majini padanho rejenome nekuratidza kutevedzana zvimiro uye zvinhu zvakachengetedza kana kusiyanisa marudzi akasiyana.Yakajairika yekuenzanisa genomics yekudzidza inosanganisira ongororo mumhuri yejini, kusimukira kwekushanduka, kudzokorodza kwegenome yakazara, kudzvanywa kwekusarudza, nezvimwe.
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Hi-C yakavakirwa Genome Assembly
Hi-C inzira yakagadzirirwa kubata chromosome kumisikidzwa nekubatanidza kuongorora kudhuze-kwakavakirwa kupindirana uye yakakwirira-kuburikidza sequencing.Kusimba kwekudyidzana uku kunofungidzirwa kuve kwakabatana zvisina kunaka nekureba kwemuviri pamakromosomes.Naizvozvo, Hi-C data inogona kutungamira kuunganidzwa, kurongeka uye kutungamira kweakaunganidzwa akateedzana mugwaro rejenome uye kusimudzira iwo pane imwe nhamba yemakromosomes.Iyi tekinoroji inopa simba kugungano re chromosome-level genome pasina huwandu hwevanhu-based genetic mepu.Yese genome imwe inoda Hi-C.
Platform: Illumina NovaSeq Platform / DNBSEQ
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Chirimwa/Mhuka De Novo Genome Sequencing
De Novokutevedzana kunoreva kuvakwa kwejenome rerudzi rwemhando yose vachishandisa matekinoroji ekutevedzanisa, semuenzaniso PacBio, Nanopore, NGS, zvichingodaro, pasina genome rereferensi.Kuvandudzwa kunoshamisa mukuverenga kureba kwechizvarwa chechitatu kutevedzana matekinoroji kwakaunza mikana mitsva mukuunganidza majenomes akaoma, akadai seaya ane heterozygosity yakakwira, chiyero chepamusoro chenzvimbo dzinodzokororwa, mapolyploid, nezvimwewo. kugadzirisa zvinhu zvinodzokororwa, matunhu ane abnormal GC zvirimo uye mamwe matunhu akaomarara.
Platform: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq Platform
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Kutevedzana Kwemunhu Yese Exome
Whole exome sequencing (WES) inoonekwa senzira inodhura-inoshanda yekuteedzera nzira yekuziva shanduko dzinokonzera chirwere.Kunyangwe maexons achingotora angangoita 1.7% yegenome yese, inomiririra chimiro chese mapuroteni anoshanda zvakananga.Mune genome yemunhu, zvakashumwa kuti inopfuura 85% yezvirwere zvinoenderana nekuchinja kunoitika munharaunda yeprotein coding.
BMKGENE inopa yakazara uye inochinjika yemunhu yese exome sequencing masevhisi ane akasiyana exon ekutora nzira dziripo dzekuzadzisa zvinangwa zvakasiyana zvekutsvaga.
Platform: Illumina NovaSeq Platform
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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, kunyanya pahuwandu hwevanhu, inhanho inokosha muzvidzidzo zvekubatana kwemajini, izvo zvinopa genetic nheyo yekushanda kwemajini ekutsvaga, evolutionary analysis, etc. Panzvimbo yekudzika kwese genome re-sequencing, kuderedza kumiririra genome sequencing (RRGS). ) inounzwa kuti ideredze mutengo wekuteedzana pasample, uku uchichengetedza kugona kwakanaka pakuwana genetic marker.Izvi zvinowanzo wanikwa nekubvisa chimedu chezvirambidzo mukati mehukuru hwakapihwa, iyo inonzi yakaderedzwa yekumiririra raibhurari (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) inzira yekuzvigadzira yega yeSNP genotyping ine kana isina referensi genome.
Platform: Illumina NovaSeq Platform
