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Genome-wide Association Analysis
Genome-wide association study (GWAS) ine chinangwa chekutsvaga mazita akasiyana-siyana (genotype) ane hukama nemaitiro chaiwo (phenotype).Chidzidzo cheGWAS chinoongorora ma genetic markers anoyambuka genome yenhamba huru yevanhu uye inofanotaura genotype-phenotype masangano kuburikidza nenhamba yekuongorora pahuwandu hwevanhu.Yakashandiswa zvakanyanya mukutsvagisa zvirwere zvevanhu uye kushanda kwemajini kumigodhi pahunhu hwakaoma hwemhuka kana zvirimwa.
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Single-nucleus RNA Sequencing
Iko kufambira mberi mukutora sero rimwe chete uye nzira yekuvaka raibhurari yega-inosanganiswa neyepamusoro-kuburikidza kutevedzana inobvumira gene expression zvidzidzo pasero-ne-sero.Iyo inogonesa yakadzama uye yakazara sisitimu yekuongorora pane yakaoma cell populations, iyo inodzivirira zvakanyanya masking yehterogeneity yavo nekutora mavhareji emasero ese.
Zvisinei, mamwe masero haana kukodzera kuitwa mu-single-sero suspension, saka dzimwe nzira dzekugadzirira sampuli - nucleus extraction kubva kumatissue inodiwa, kureva kuti, nucleus inotorwa zvakananga kubva mumatishu kana sero uye inogadzirirwa mu-single-nucleus suspension for single- cell sequencing.
BMK inopa gumi × Genomics Chromium TM yakavakirwa imwe-sero RNA sequencing sevhisi.Iyi sevhisi yakashandiswa zvakanyanya muzvidzidzo pazvidzidzo zvine chekuita nechirwere, senge immune cell kusiyanisa, tumor heterogeneity, kukura kwetishu, nezvimwe.
Spatial transcriptome chip: 10 × Genomics
Platform: Illumina NovaSeq 6000
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Chirimwa/Mhuka Yese Genome Sequencing
Yese genome re-sequencing, inozivikanwawo seWGS, inogonesa kuburitswa kwezvose zvakajairika uye zvisingawanzo shanduko pajenome rese kusanganisira Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), uye Copy Number Variation (CNV). )SVs inogadzira chikamu chikuru chekusiyana kwekusiyana kweSNPs uye ine simba guru pane genome, iyo ine simba guru pane zvipenyu.Kuverengera-kurebesa requencing kunobvumira kunyatsozivikanwa kwezvimedu zvakakura uye misiyano yakaoma nekuti kuverenga kwenguva refu kunoita kuti zvive nyore kuyambuka chromosomal kuyambuka matunhu akaoma senge tandem kudzokorora, GC / AT-akapfuma matunhu, uye hyper-anosiyana matunhu.
Platform: Illumina, PacBio, Nanopore
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BMKMANU S1000 Spatial Transcriptome
Kurongeka kwenzvimbo yemaseru kunoita basa rakakosha mumaitiro akasiyana-siyana ebhayoloji, sekupinzwa kwemuviri, kukura kwe embryo, zvichingodaro. Spatial transcriptome sequencing, iyo inoratidza gene expression profiling uchichengeta ruzivo rwechinzvimbo, inopa ruzivo rwakakura mu transcriptome-level tissue architecture.Nekuvandudza tekinoroji, iyo yekupedzisira-yakajeka tishu morphology uye chaiyo dhizaini musiyano we spatial molecular expression inoda kudzidzwa nepamusoro resolution.BMKGENE inopa yakazara, imwe-stop spatial transcriptome sequencing sevhisi kubva kumasample kuenda kune biological ruzivo.
Spatial transcriptomics tekinoroji inogonesa maonero matsva munhandare yekutsvagisa dzakasiyana nekugadzirisa gene expression profil ine spatial yemukati mune heterogenous samples.
Spatial transcriptome chip: BMKMANU S1000
Platform: Illumina NovaSeq 6000
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10x Genomics Visium Spatial Transcriptome
Visium Spatial Gene Expression ndiyo yakajairwa spatial transcriptome sequencing tekinoroji yekuisa mumhando yematishu zvichienderana nemRNA yakazara.Mepu iyo yese transcriptome ine morphological mamiriro kuti uwane mitsva yeruzivo mukukura kwakajairwa, chirwere chechirwere, uye kiriniki yekududzira tsvakiridzo.BMKGENE inopa yakazara, imwe-stop spatial transcriptome sequencing sevhisi kubva kumasample kuenda kune biological ruzivo.
Spatial transcriptomics tekinoroji inogonesa maonero matsva munhandare dzakasiyana siyana dzekutsvagisa nekugadzirisa gene expression profil ine spatial yemukati mune heterogenous samples..
Spatial transcriptome chip: 10x Genomics Visium
Platform:Illumina NovaSeq 6000
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Yakazara-Kureba mRNA Sequencing-Nanopore
RNA sequencing yanga iri chishandiso chakakosha chekuongorora kwakadzama transcriptome.Pasina kupokana, yechinyakare-pfupi-kuverenga kutevedzana yakawana yakawanda yakakosha budiriro muno.Zvakangodaro, inowanzosangana nemipimo mune yakazara-kureba isoform kuzivikanwa, quantification, PCR kusarura.
Nanopore sequencing inozvisiyanisa kubva kune mamwe mapuratifomu ekutevedzana, pakuti nucleotides inoverengwa zvakananga pasina DNA synthesis uye inogadzira kuverenga kwenguva refu pamakumi e kilobases.Izvi zvinopa simba rakananga kuverenga-kunze kuyambuka yakazara-kureba zvinyorwa uye kugadzirisa zvinonetsa muisoform-level zvidzidzo.
Platform:Nanopore PromethION
Raibhurari:cDNA-PCR
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Yakazara-kureba mRNA kutevedzana -PacBio
De novofull-length transcriptome sequencing, inozivikanwawo seDe novoIso-Seq inotora zvakanakira PacBio sequencer mukureba kwekuverenga, iyo inogonesa kutevedzana kweakazara-kureba cDNA mamorekuru pasina chero mabreak.Izvi zvinodzivirira zvachose chero zvikanganiso zvinogadzirwa mumatanho egungano ezvinyorwa uye zvinovaka unigene seti ine isoform-level resolution.Iyi unigene seti inopa ruzivo rwakasimba rwemajini se "reference genome" pa transcriptome-level.Uye zvakare, kusanganisa nechizvarwa chinotevera chekuteedzera data, iyi sevhisi inopa iyo chaiyo quantification yeisoform-level kutaura.
Platform: PacBio Sequel IIRaibhurari: SMRT bhero raibhurari -
Eukaryotic mRNA Sequencing-Illumina
mRNA sequencing inogonesa profiling yeese mRNAs akanyorwa kubva kumaseru pasi pemamiriro chaiwo.Iyo tekinoroji ine simba yekuburitsa chimiro chejeni, zvimiro zvemajini uye mamorekuru emamwe maitiro ebhayoloji.Parizvino, kutevedzana kwemRNA kwave kushandiswa zvakanyanya mukutsvaga kwakakosha, kuongororwa kwekiriniki, kukura kwezvinodhaka, nezvimwe.
Platform: Illumina NovaSeq 6000
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Non-Reference based mRNA Sequencing-Illumina
mRNA sequencing inotora inotevera-chizvarwa kutevedzana maitiro (NGS) kutora mutumwa RNA(mRNA) fomu Eukaryote panguva chaiyo iyo mamwe mabasa akakosha ari kuita.Chinyorwa chakareba kwazvo chakanatswa chainzi 'Unigene' uye chakashandiswa sereferensi yekuongorora kunotevera, inova nzira inoshanda yekudzidza mamorekuru nemambure ekutonga emarudzi pasina kutaurwa.
Mushure mekubatanidza data yetranscriptome uye unigene inoshanda annotation
(1) Ongororo yeSSR, kufanotaura kweCDS uye chimiro chemajini zvichafanogadzirwa.
(2) Quantification ye unigene kutaura mumuenzaniso wega wega uchaitwa.
(3) Unigenes akasiyaniswa pakati pemasamples (kana mapoka) achawanikwa zvichibva pane unigene kutaura.
(4) Kubatanidza, chirevo chekushanda uye kuvandudza kuongororwa kweunigenes yakasiyana-siyana ichaitwa.
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Yakareba isiri-coding kutevedzana-Illumina
Marefu asina kukodha maRNA (lncRNAs) imhando yemamorekuru eRNA ane hurefu hunodarika 200 nt, ayo anoratidzirwa nekuderera zvakanyanya kugona kukodha.LncRNA, senhengo yakakosha mune isiri-coding RNAs, inonyanya kuwanikwa munyucleus uye plasma.Iko kusimukira mukuteedzera tekinoroji uye bioinformatics inogonesa kuzivikanwa kweakawanda novel lncRNAs uye kubatanidza ayo ane biological mabasa.Uchapupu hunowedzera hunoratidza kuti lncRNA inonyanya kubatanidzwa mu epigenetic regulation, transcription regulation uye post-transcription regulation.
