Metatranscretprome kutevedzana
Service maficha
● RRN Flight inoteverwa nekutungamira mrna library kugadzirira.
● Kuongorora kuIllumina novaseq.
Zvigadzirwa zvebasa
●Dzidza shanduko dzechikoro chezvakaipa zve microbial:Izvi zvinoitika padanho rekushandurwa uye kuongorora zvingangoita genera ratsva.
●Kutsanangurira microbial nharaunda yekudyidzana nehutachiona kana nharaunda.
●Yakarongedzwa Bioinformatic Analysis: Izvi zvinopa ruzivo rwekunzwisisika pangozi venhamba uye zvinoshanda makomendi, pamwe nekusiyanisa kwerugwaro rweGene kuongororwa.
●Yakawedzera gene gentation:Uchishandisa kumusoro-to-date gene basa reDhatabhesi yeGumi Expression Ruzivo rwezvikoro zveMachinda.
●Post-Kutengesa Tsigiro:Kuzvipira kwedu kunowedzera kupfuura purojekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa basa nguva. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
Service Rudzidziso
| Sequencing Puratifomu | Kuongorora zano | Data yakakurudzirwa | Data Mhando Dzidzo |
| Illumina Nevaaseq | Pe150 | 12GB | Q3085% |
Zvinodiwa Muenzaniso
| Concentration (ng / μL) | Mari Yese (μG) | Vhoriyamu (μL) | Od260 / 280 | Od260 / 230 | Rin |
| ≥50 | ≥1.0 | ≥20 | 1.8-2.0 | 1.0-2.5 | ≥6.5 |
Basa rebasa rinoyerera
Muenzaniso
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Inosanganisira kuongororwa kunotevera:
● SEQUENCET DATA YEMAHARA DZESVONDO
● Kosi yeKumusoro
● Taxonomic Annotation uye zvakawanda
● Inoshanda Annotation uye zvakawanda
● Chirevo quature uye kusiyanisa kuongorora
Taxoniomic kugoverwa kweumwe neumwe muenzaniso:
Beta Diversity Analysis: Upgma
Inoshanda Annotation - Enda kuwanda
Musiyanisa Terkomy Wakawanda - Lefse
Ongorora kufambira mberi kwakabatana neBMKGEDZIDZO YEMADZIDZO YEMAHARA SEXQUCE MABHUKU KUNYANYA KUNYANYA KWEMAHARA.
Lu, z. et al. .Wutrition yemhuka, 14, p. 130-140. Doi: 10.1016 / J.aninu.2023.05.006.
Rwiyo, Z. Et Al. . "Mbiru muzvikoro zveMicrobiology, 8 (Jul). Doi: 10.3389 / ficb.2017.01294 / izere.
Wang, W. et Al. .Viramu, 14 (11), p. 2552. Dii: 10.390 / v14112552 / S1.
Wei, J. et al. . "Molekyar Echology, 31 (15), pp. 3999-016. Doi: 10.1111 / mec.16557.
