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Metatranscriptome Sequencing

Leveraging Illumina sequencing tekinoroji, BMKGENE's metaranscriptome sequencing sevhisi inovhura iyo inochinja yejini kuratidzwa kweakasiyana siyana ehutachiona, kutenderera eukaryotes kune prokaryotes uye mavhairasi, mukati mezvakatipoteredza nharaunda senge ivhu, mvura, gungwa, chituru, uye ura. Sevhisi yedu yakazara inopa simba vaongorori kuti vatarise munharaunda dzakaomarara dzenharaunda 'dzizere gene expression profiles. Kupfuura ongororo yetaxonomic, yedu metaranscriptome sequencing sevhisi inofambisa ongororo mukupfumisa inoshanda, ichivhenekera kujekeswa kwemajini anoratidzwa zvakasiyana uye mabasa awo. Vhura hupfumi hweruzivo rwebhayoloji paunenge uchifamba munzvimbo dzakaoma dzemajeni ekutaura, mutsauko wetaxonomic, uye mashandiro anoita mukati meaya akasiyana niches ezvakatipoteredza.

Taura nesu

Service Details

Bioinformatics

Demo Results

Zvinyorwa zvinoratidzwa

Zvimiro Zvebasa

● rRNA kupera kunoteverwa nedirectional mRNA library library.

● Sequencing paIllumina NovaSeq.

Basa Rinobatsira

●Dzidza Shanduko dzeComplex Microbial Communities:Izvi zvinoitika padanho rekunyora uye kuongorora zvingangoita majini matsva.

●Kutsanangura Kudyidzana Kwenharaunda yeMicrobial neMugamuchiri kana Nzvimbo.

●Comprehensive Bioinformatic Analysis: Izvi zvinopa manzwisisiro panharaunda taxonomic uye inoshanda maumbirwo, pamwe nekusiyanisa gene expression analysis.

●Yakakura Gene Annotation:Kushandisa up-to-date gene function dhatabhesi yeruzivo rwemajeni ekutaura ruzivo rwenharaunda dzine hutachiona.

●Post-Sales Support:Kuzvipira kwedu kunowedzera kupfuura kupera kweprojekiti nemwedzi mitatu mushure mekutengesa sevhisi nguva. Munguva ino, tinopa kutevedza kweprojekiti, rubatsiro rwekugadzirisa matambudziko, uye maQ&A masesheni kugadzirisa chero mibvunzo ine chekuita nemhedzisiro.

Sevhisi Zvinotsanangurwa

Sequencing chikuva	Sequencing Strategy	Data inokurudzirwa	Data Quality Control
Illumina NovaSeq	PE150	12Gb	Q30≥85%

Muenzaniso Zvinodiwa

Concentration (ng/µL)	Huwandu hwese (µg)	Vhoriyamu (µL)	OD260/280	OD260/230	RIN
≥50	≥1.0	≥20	1.8-2.0	1.0-2.5	≥6.5

Kuyerera Kwebasa Rebasa

Muenzaniso wekutumira

Kuvakwa kweraibhurari

Sequencing

Data analysis

Mushure mekutengesa mabasa

Zvakapfuura: De novo Fungal Genome Assembly

Zvinotevera: Prokaryotic RNA Sequencing

Inosanganisira ongororo inotevera:

● Sequencing Data Quality Control

● Gwaro Rekunyora

● Taxonomic Annotation uye Kuwanda

● Functional Annotation uye Kuwanda

● Kutaura Quantification uye Differential Analysis

Taxonomic kugoverwa kwemuenzaniso wega wega:

Beta siyana ongororo: UPGMA

Functional annotation - GO kuwanda

Yakasiyana taxonomy kuwanda - LEFSE

Ongorora kufambira mberi kunofambiswa neBMKGene's meta transcriptomics sequencing masevhisi kuburikidza neakaunganidzwa muunganidzwa wezvinyorwa.

Lu, Z. nevamwe. (2023) 'Acid kushivirira kwelactate-kushandisa mabhakitiriya ehurongwa Bacteroidales inobatsira mukudzivirira ruminal acidosis mumbudzi yakagadziridzwa kune yakanyanya-concentrate kudya',Kudya Kwemhuka, 14, mapeji 130–140. doi: 10.1016/J.ANINU.2023.05.006.

Rwiyo, Z. et al. (2017) 'Kusunungura musimboti unoshanda microbiota mune yechinyakare yakasimba-nyika kuviriswa nepamusoro-kuburikidza amplicon uye metaranscriptomics sequencing',Frontiers muMicrobiology, 8(JUL). doi: 10.3389/FMICB.2017.01294/FULL.

Wang, W. et al. (2022) 'Novel Mycoviruses Yakawanikwa kubva kuMetatranscriptomics Survey yePhytopathogenic Alternaria Fungus',Mavhairasi, 14(11), p. 2552. doi: 10.3390/V14112552/S1.

Wei, J. nevamwe. (2022) 'Parallel metatranscriptome analysis inoratidza kushatiswa kwechirimwa chechipiri metabolites nezvipembenene nematumbu avo',Molecular Ecology, 31(15), mapeji 3999–4016. doi: 10.1111/MEC.16557.