mRNA-seq (NGS) ine genome reference
RNA-seq chishandiso chakajairika muzvidzidzo zvehupenyu nezvirimwa, ichivhara musiyano uripo pakati pema genomes nema proteomes. Simba rayo riri mukuwana zvinyorwa zvitsva uye kuyedza kutaurwa kwazvo muongororo imwe chete. Inoshandiswa zvakanyanya muzvidzidzo zvekuenzanisa transcriptomic, ichijekesa majini ane chekuita nehunhu hwakasiyana kana phenotypes, sekuenzanisa mutants nemarudzi emusango kana kuratidza kutaurwa kwemajini pasi pemamiriro chaiwo. BMKCloud mRNA(Reference) APP inobatanidza quantification yekutaura, differential expression analysis(DEG), uye sequence structure analysis mu mRNA-seq(NGS) bioinformatics pipeline uye inobatanidza simba re software yakafanana, ichivimbisa kuti zviri nyore uye zviri nyore kushandisa. Vashandisi vanogona kurodha data ravo reRNA-seq ku cloud, uko App inopa mhinduro yakazara, imwe chete yekuongorora bioinformatic. Pamusoro pezvo, inokoshesa ruzivo rwevatengi, ichipa mashandiro akagadzirirwa zvinodiwa nevashandisi. Vashandisi vanogona kuisa ma parameter uye kutumira pipeline mission vega, kutarisa mushumo wekudyidzana, kuona data/diagrams uye kupedzisa data mining, senge: target gene selection, functional clustering, diagramming, nezvimwewo.
Puratifomu:Illumina, MGI
Maitiro:RNA-Seq
Marongerwo: Data rakachena, rakacheneswa.
Rudzi rweraibhurari:fr-unstranded, fr-firststrand kana fr-secondstrand
Kureba kwekuverenga:150 bp
Rudzi rwefaira:*.fastq, *.fq, *.fastq.gz kana *.fq.gz. Sisitimu iyi ichaitabatanidza otomatiki mafaira e.fastq zvichienderana nemazita emafaira avo,semuenzaniso *_1.fastq yakabatana ne *._2.fastq.
Huwandu hwemienzaniso:Hapana zvirambidzo panhamba yachoyemienzaniso, asi nguva yekuongorora ichawedzera sezvo nhamba yemienzaniso inokura.
Huwandu hweData Hunokurudzirwa:6G pamuenzaniso wega wega
