Fungal Genome

Biomarker Technologies inopa genome ongororo, yakanaka genome uye pene-yakazara genome yefungal zvichienderana nechinangwa chekutsvaga.Genome sequencing, kuungana uye kushanda annotation inogona kuwanikwa nekubatanidza Inotevera-chizvarwa kutevedzana + Yechitatu chizvarwa kutevedzana kuti iwane yakakwirira-level genome ungano.Hi-C tekinoroji inogona zvakare kushandiswa kufambisa genome kusangana pakromosome level.

Platform:PacBio Sequel II

Nanopore PromethION P48

Illumina NovaSeq Platform

Taura nesu

Service Details

Demo Results

Basa Rinobatsira

● Nzira dzakawanda dzekutevedzana dziripo dzezvinangwa zvekutsvaga zvakasiyana

● Ane ruzivo rwakanyanya mukusangana kwefungi genome nepamusoro pe10,000 pene-complete genome yakaunganidzwa.

● Nyanzvi mushure mekutengesa tekinoroji yetsigiro timu inozadzisa zvakanangana tsvakiridzo zvinodiwa.

Sevhisi Zvinotsanangurwa

Service	Sequencing Strategy	Hunhu hunovimbiswa	Inofungidzirwa kutendeuka-nguva
Fungal yakanaka mepu	Illumina 50X+Nanopore 100X	Contig N50≥2 Mb	35 mazuva ekushanda
Fungal yakanaka mepu	PacBio HiFi 30X	Contig N50≥2 Mb	35 mazuva ekushanda
Fungal pene-yakazara mepu	Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	Chromosome anchoring ratio>90%	45 mazuva ekushanda

Bioinformatics inoongorora

● Raw data quality control

● Gungano reGenome

● Genome chikamu chekuongorora

● Gene basa rondedzero

● Kuenzanisa genomic kuongorora

Sample Zvinodiwa uye Delivery

Sample Zvinodiwa:

ForDNA zvinyorwa:

Muenzaniso Type	Mari	Concentration	Kuchena
DNA zvinyorwa	＞ 1.2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Kune matishu samples:

Muenzaniso mhando

Yakakurudzirwa kurapwa kwemuenzaniso

Sample kuchengetedza uye kutumira

Unicellular Fungus

Tarisa mbiriso pasi pemaikorosikopu uye uvaunganidze muchikamu chavo cheexponential

Shandura tsika (ine approx. 3-4.5e9 masero) mu 1.5 kana 2 ml eppendorf.(Ramba uri muchando)

Centrifuge chubhu ye1 min pa14000 g kuunganidza mabhakitiriya uye kubvisa supernatant nekungwarira.

Nama chubhu uye omesa mabhakitiriya mune mvura nitrogen kweinenge 1-3 h.Chengetedza chubhu mu -80 ℃ firiji.

Svitsa masampula munitrogen yemvura kwemaawa 3-4 uye chengetedza mune yemvura nitrogen kana -80 dhigirii kusvika kurebesa kwenguva refu.Sample shipping with dry-ice inodiwa.

Macro Fungus

Tissue muchikamu chekukura chinokurudzirwa.

Sukurudza matishu nemvura isina endotoxin, wozoita 70% ethonal.

Chengetedza sampuli mu cryo-tubes.