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Chromatin Immunoprecipitation Sequencing (ChIP-seq)
ChIP-Seq provides genome-wide profiling of DNA targets for histone modification, transcription factors, and other DNA-associated proteins. It combines the selectivity of chromatin immuno-precipitation (ChIP) to recover specific protein-DNA complexes, with the power of next-generation sequencing (NGS) for high-throughput sequencing of the recovered DNA. Additionally, because the protein-DNA complexes are recovered from living cells, binding sites can be compared in different cell types and tissues, or under different conditions. Applications range from transcriptional regulation to developmental pathways to disease mechanisms and beyond.
Platform: Illumina NovaSeq 6000
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Whole genome bisulfite sequencing
DNA methylation at the fifth position in cytosine (5-mC) has a fundamental influence on gene expression and cellular activity. Abnormal methylation patterns have been associated with several conditions and diseases, such as cancer. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.
Platform: Illumina NovaSeq6000
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Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
ATAC-seq is a high-throughput sequencing method for analysis of genome-wide chromatin accessibility, which is important for global epigenetic control of gene expression. Sequencing adapters are inserted into open chromatin regions by hyperactive Tn5 transposase. After PCR amplification, a sequencing library is constructed. All the open chromatin regions can be obtained under a specific space-time condition, not only limited to the binding sites of a transcription factor, or a certain histone modified region.
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Reduced Representation Bisulfite Sequencing (RRBS)
DNA methylation research has always been a hot topic in disease research, and is closely related to gene expression and pheno-typic traits. RRBS is an accurate, efficient and economical method for DNA methylation research. Enrichment of promoter and CpG island regions by enzymatic cleavage (Msp I), combined with Bisulfite sequencing, provides high resolution DNA methylation detection.
Platform: Illumina NovaSeq 6000
