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Transcriptomics

  • Full-length mRNA sequencing-Nanopore

    Full-length mRNA sequencing-Nanopore

    RNA sequencing has been an invaluable tool for comprehensive transcriptome analysis. Doubtlessly, traditional short-read sequencing achieved numerous important development in here. Nevertheless, it often encounters limitations in full-length isoform identifications, quantification, PCR bias.

    Nanopore sequencing distinguishes itself from other sequencing platforms, in that the nucleotides are read directly without DNA synthesis and generates long read at tens of kilobases. This empowers direct read-out crossing full-length transcripts and tackling the challenges in isoform-level studies.

    PlatformNanopore PromethION

    Library: cDNA-PCR

  • De novo Full-length Transcriptome sequencing -PacBio

    De novo Full-length Transcriptome sequencing -PacBio

    De novo full-length transcriptome sequencing, also known as De novo Iso-Seq takes the advantages of PacBio sequencer in read length, which enables sequencing of full-length cDNA molecules without any breaks. This completely avoids any errors generated in transcript assembly steps and constructs unigene sets with isoform-level resolution. This unigene sets provides powerful genetic information as “reference genome” at transcriptome-level. In addition, combining with next generation sequencing data, this service empowers an accurate quantification of isoform-level expression.

    Platform: PacBio Sequel II
    Library: SMRT bell library
  • Eukaryotic mRNA sequencing-Illumina

    Eukaryotic mRNA sequencing-Illumina

    mRNA sequencing enables profiling of all mRNAs transcripted from cells under specific conditions. It is a powerful technology for revealing gene expression profile, gene structures and molecular mechanisms of certain biological processes. To date, mRNA sequencing has been widely employed in fundamental research, clinical diagnostics, drug development, etc.

    Platform: Illumina NovaSeq 6000

  • Non-Reference based mRNA sequencing-Illumina

    Non-Reference based mRNA sequencing-Illumina

    mRNA sequencing adopts next-generation sequencing technique (NGS) to capture the messenger RNA(mRNA) form Eukaryote at specific period that some special functions are activating. The longest transcript spliced was called ‘Unigene’ and used as the reference sequence for subsequent analysis, which is an effective means to study the molecular mechanism and regulatory network of the species without reference.

    After transcriptome data assembly and unigene functional annotation

    (1)SNP analysis, SSR analysis, CDS prediction and gene structure will be preformed.

    (2)Quantification of unigene expression in each sample will be performed.

    (3)Differentially expressed unigenes between samples (or groups) will be discovered based on unigene expression

    (4)Clustering, functional annotation and enrichment analysis of differentially expressed unigenes will be performed

  • Long non-coding sequencing-Illumina

    Long non-coding sequencing-Illumina

    Long non-coding RNAs (lncRNAs) are a type of RNA molecules with length exceeding 200 nt, which are characterize by extremely low coding potential. LncRNA, as a key member in non-coding RNAs, is mainly found in nucleus and plasma. The development in sequencing technology and bioinformtics enables identification of numerous novel lncRNAs and associate those with biological functions. Accumulative evidences suggest that lncRNA is widely involved in epigenetic regulation, transcription regulation and post-transcription regulation.

  • Small RNA sequencing-Illumina

    Small RNA sequencing-Illumina

    Small RNA refers to a class of non-coding RNA molecules that are usually less than 200nt in length, including micro RNA (miRNA), small interference RNA (siRNA), and piwi-interacting RNA (piRNA).

    MicroRNA (miRNA) is a class of endogenous small RNA with a length of about 20-24nt, which plays a variety of important regulatory roles in cells. miRNA involved in many life processes which revealing tissue – specific and stage – specific expression and highly conserved in different species.

  • circRNA sequencing-Illumina

    circRNA sequencing-Illumina

    Whole transcriptome sequencing is designed to profile all types of RNA molecules, including coding (mRNA) and non-coding RNAs (including lncRNA, circRNA and miRNA) which are transcribed by specific cells at a certain time. Whole transcriptome sequencing, also known as “total RNA sequencing” aims at revealing comprehensive regulatory networks at transcriptome level. Taking advantage of NGS technology, sequences of entire transcriptome products are available for ceRNA analysis and joint RNA analysis, which provides the first step towards functional characterization. Revealing regulatory network of circRNA-miRNA-mRNA based ceRNA.

  • Whole transcriptome sequencing – Illumina

    Whole transcriptome sequencing – Illumina

    Whole transcriptome sequencing is designed to profile all types of RNA molecules, including coding (mRNA) and non-coding RNAs (including lncRNA, circRNA and miRNA) which are transcribed by specific cells at a certain time. Whole transcriptome sequencing, also known as “total RNA sequencing” aims at revealing comprehensive regulatory networks at transcriptome level. Taking advantage of NGS technology, sequences of entire transcriptome products are available for ceRNA analysis and joint RNA analysis, which provides the first step towards functional characterization. Revealing regulatory network of circRNA-miRNA-mRNA based ceRNA.

  • Prokaryotic RNA sequencing

    Prokaryotic RNA sequencing

    Prokaryotic RNA sequencing uses next generation sequencing (NGS) to reveal the presence and quantity of RNA at a given moment, by analyzing the changing cellular transcriptome. Our company‘s prokaryotic RNA sequencing, specifically aims at prokaryotes with reference genomes, providing you with transcriptome profiling, gene structure analysis, etc. It has been widely applied to basic science research, drug research and development, and more.

    Platform: Illumina NovaSeq 6000

  • Metatranscriptome Sequencing

    Metatranscriptome Sequencing

    Metatranscriptome sequencing indentifies gene expression of microbes (both eukaryotes and prokaryotes) within natural enviroments (i.e. soil, water, sea, feces, and gut.).Specifically, this services allows you to obtain whole gene expression profiling of complex microbial communities, taxonomic analysis of species, functional enrichment analysis of differently expressed genes, and more.

    Platform: Illumina NovaSeq 6000

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