De novo sequencing refers to construction of a species’ whole genome using sequencing technologies, e.g. PacBio, Nanopore, NGS, etc., in absence of a reference genome. The remarkable improvement in read length of third generation sequencing technologies has brought new opportunities in assembling complex genomes, such as those with high heterozygosity, high ratio of repetitive regions, polyploids, etc. With read length at tens of kilobases level, these sequencing reads enable resolving of repetitive elements, regions with abnormal GC contents and other highly complex regions.
Platform： PacBio Sequel II /Nanopore PromethION P48/ Illumina NovaSeq6000