circRNA sequencing-Illumina
Features
● rRNA kupera kunoteverwa negwara rekugadzirira raibhurari, zvichiita kuti kutevedzana kwedata kune strand-chaiyo.
● Bioinformamatic workflow inogonesa circRNA kufanotaura uye kutaura quantification
Basa Rinobatsira
●Mamwe mabhuku eRNA akazara:Isu tinoshandisa rRNA kupera pachinzvimbo chekuderera kweRNA mumutsara mukugadzirira kwedu rekutanga raibhurari, kuve nechokwadi chekuti data rekuteedzera haringosanganisire circRNA chete asiwo mRNA nelncRNA, ichigonesa kuongororwa kwakabatana pane aya dataset.
●Optional ongororo yemakwikwi endogenous RNA (ceRNA) network: kupa nzwisiso yakadzama mumaserura ekudzora maitiro
●Hunyanzvi Hwakawanda: iine rekodhi rekodhi rekugadzirisa anopfuura zviuru makumi maviri esampuli paBMK, inotora akasiyana emhando dzemhando uye mapurojekiti elncRNA, timu yedu inounza hupfumi hweruzivo kupurojekiti yega yega.
●Rigorous Quality Control: Isu tinoshandisa epakati kudzora mapoinzi pamatanho ese, kubva kumuenzaniso uye kugadzirira raibhurari kune kutevedzana uye bioinformatics.Uku kutarisisa kwakadzama kunovimbisa kuburitswa kwezvinogara zvemhando yepamusoro mhedzisiro.
● Post-Sales Support: Kuzvipira kwedu kunowedzera kupfuura kupera kweprojekiti nemwedzi mitatu mushure mekutengesa sevhisi nguva.Munguva ino, tinopa kutevedza kweprojekiti, rubatsiro rwekugadzirisa matambudziko, uye maQ&A masesheni kugadzirisa chero mibvunzo ine chekuita nemhedzisiro.
Sample Zvinodiwa uye Delivery
| Library | Platform | Yakakurudzirwa data | Data QC |
| Poly A yakafumiswa | Illumina PE150 | 16-20 Gb | Q30≥85% |
Sample Zvinodiwa:
Nucleotides:
| Conc.(ng/μl) | Mari (μg) | Kuchena | Kuperera |
| ≥ 100 | ≥ 0.5 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Yakaganhurirwa kana isina mapuroteni kana kusvibiswa kweDNA kunoratidzwa pagel. | Kumiti: RIN≥6.5; Zvemhuka: RIN≥7.0; 5.0≥28S/18S≥1.0; kukwirira kwakadzika kana kusina kwekutanga |
● Zvirimwa:
Mudzi, Dzinde kana Petal: 450 mg
Shizha kana Mbeu: 300 mg
Muchero: 1.2 g
● Mhuka:
Mwoyo kana Ura: 450 mg
Viscera kana Brain: 240 mg
Tsandanyama: 600 mg
Mabhonzo, Bvudzi kana Ganda: 1.5g
● Arthropods:
Zvipembenene: 9g
Crustacea: 450 mg
● Ropa rose:2 machubhu
● Masero: 106 masero
● Serum uye Plasma: 6 mL
Yakakurudzirwa Sample Delivery
Container: 2 ml centrifuge chubhu (Tin foil haina kukurudzirwa)
Sample labeling: Boka+ replicate eg A1, A2, A3;B1, B2, B3...
Shipment:
1. Dry-ice: Samples dzinoda kurongedzerwa mumabhegi uye kuvigwa muoma-aizi.
2. RNAstable tubes: RNA samples inogona kuomeswa muRNA stabilization tube (eg RNAstable®) uye inotumirwa mukamuri yekupisa.
Kuyerera Kwebasa Rebasa
Kuedza kugadzira
Muenzaniso wekutumira
RNA kubviswa
Kuvakwa kweraibhurari
Sequencing
Data analysis
Mushure mekutengesa masevhisi
Bioinformatics
circRNA kufanotaura: kugovera kwechromosomal
Differentially Inoratidzwa circRNAs - Volcano plot
Differentially Expressed circRNAs - hierarchical clustering
Kushanda kupfumisa kwe circRNA's host genes
Ongorora kufambira mberi kwekutsvagisa kunofambiswa neBMKGene 'circRNA sequencing masevhisi kuburikidza neakaunganidzwa muunganidzwa wezvinyorwa.
Wang, X nevamwe.(2021) 'CPSF4 inodzora circRNA kuumbwa uye microRNA mediated gene silence mu hepatocellular carcinoma', Oncogene 2021 40:25, 40 (25), mapeji 4338-4351.doi: 10.1038/s41388-021-01867-6.
Xia, K. et al.(2023) 'X oo-responsive transcriptome inoratidza basa reRNA133 inotenderera mukudzivirira zvirwere nekugadzirisa kutaura kweOsARAB mumupunga', Phytopathology Research, 5 (1), mapeji 1-14.doi: 10.1186/S42483-023-00188-8/FIGURES/6.
Y, H. nevamwe.(2023) 'CPSF3 inogadzirisa chiyero chedenderedzwa uye mutsara zvinyorwa muhepatocellular carcinoma'.doi: 10.21203/RS.3.RS-2418311/V1.
Zhang, Y. et al.(2023) 'Kuongorora kwakazara kwe circRNAs mu cirrhotic cardiomyopathy isati yatanga uye mushure mekutapurirwa kwechiropa', International Immunopharmacology, 114, p.109495. doi: 10.1016/J.INTIMP.2022.109495.
