PHEPO PHETOHO
liphatsa tsa lefutso tsa tlhaho
Kopano ea boleng bo holimo ea genome e totobatsa litšobotsi tsa rye genomic le liphatsa tsa lefutso tsa bohlokoa tsa temo
PacBio |Ilumina |Bionano Optical Map |Kopano ea Hi-C Genome |'Mapa oa liphatsa tsa lefutso |Li-sweeps tse Khethehileng |RNA-Seq |ISO-seq |SLAF-seq
Biomarker Technologies e fane ka tšehetso ea tekheniki mabapi le tatellano ea Pacbio, tatellano ea Hi-C le tlhahlobo ea data thutong ena.
Lintlha-khōlō
1.Ho ile ha fumanoa mofuta oa pele oa boemo bo phahameng ba chromosomal Rye genome, e nang le boholo ba chromosome bo fetang 1 Gb.
2.Ha ho bapisoa le Tu, Aet le Hv genome, liketsahalo tse ikhethang tsa morao-rao tsa LTR-RT li ile tsa bonoa ho Rye genome, e neng e ikarabella bakeng sa ho atolosoa ha boholo ba genome ea rye.
3. Phapang pakeng tsa rye le koro ea diploid e etsahetse ka mor'a ho arohana ha harese ho koro, ka linako tse fapaneng bakeng sa liketsahalo tse peli tse ka bang 9.6 le 15 MYA.
FT liphatsa tsa lefutso phosphorylation e ka laola mokhoa oa pele oa sehlooho ho rye.
4. Tlhahlobo e ikhethileng e bonts'a ho kenya letsoho ha ScID1 taolong ea letsatsi la sehlooho le khetho ea eona e ka bang teng ka ho ruisa rye.
Ka morao
Ka morao
Rye ke sejalo sa bohlokoa sa lijo le furu, mohloli oa bohlokoa oa liphatsa tsa lefutso bakeng sa ntlafatso ea koro le triticale, le thepa ea bohlokoa bakeng sa lithuto tse bapisang tsa genomic tsa joang.Weining rye, mofuta oa lipalesa oa pele o lengoang Chaena, o tsoteha ka lebaka la ho hanyetsana le hlobo ka bongata le mafome a mela.Ho utloisisa motheo oa liphatsa tsa lefutso le limolek'hule tsa litšobotsi tse phahameng tsa rye le ho khothaletsa lithuto tsa genomic le ho ikatisa ho rye le lijalo tse amanang le tsona, mona re hlophisitse le ho sekaseka genome ea Weining rye.
Katleho
Rye Genome
Rye genome e hahiloe ka ho kopanya lipalo tsa PacBio SMRT, tatellano e khutšoane ea Illumina, hammoho le tse tsoang ho chromatin conformation capture (Hi-C), 'mapa oa liphatsa tsa lefutso, le tlhahlobo ea BioNano.Li-contigs tse kopaneng (7.74 Gb) li nkile karolo ea 98.47% ea boholo bo hakantsoeng ba genome (7.86 Gb), 'me 93.67% ea li-contigs (7.25 Gb) e abetsoe li-chromosome tse supileng.Lintho tse pheta-phetoang li ne li etsa 90.31% ea genome e kopaneng.
Rye Genome
'Mapa oa khokahano ea liphatsa tsa lefutso (WJ) o ntlafalitsoe ho sebelisoa limela tse 295 tsa F2 tse nkiloeng ho tšela mabala a mabeli a rye (Weining × Jingzhou)
'Mapa oa ho ikopanya oa Hi-C oa li-chromosome tse supileng tse kopaneng tsa Weining rye (1R - 7R)
Khokahano lipakeng tsa li-chromosome tse supileng tse kopaneng tsa Weining rye le lihlopha tse supileng tsa khokahano ea rye tse ntlafalitsoeng ka Lo7 x Lo255 RIL
LTR Assembly Index (LAI) boleng ba Rye genome e fumanoe e le 18.42 le 1,393 (96.74%) ea liphatsa tsa lefutso tsa BUSCO tse 1,440 tse bolokiloeng haholo. le libaka tsa tlhaho.Kakaretso ea liphatsa tsa lefutso tsa protheine tse 86,991, ho kenyeletsoa liphatsa tsa lefutso tse 45,596 tse nang le ts'epo e phahameng (HC) le liphatsa tsa lefutso tse 41,395 tse nang le ts'epo e tlase (LC) li ile tsa boleloa esale pele.
2. Tlhahlobo ea li-TE
Tlhahlobo ea TEs.Kakaretso ea 6.99 Gb, e emelang 90.31% ea kopano ea Weining, e ile ea hlalosoa e le TEs, e kenyeletsang likarolo tse 2,671,941 tsa malapa a 537.Litaba tsena tsa TE li ne li le holimo ka ho hlakileng ho feta tse tlalehiloeng pele bakeng sa Ta (84.70%), Tu (81.42%), Aet (84.40%), WEW (82.20%), kapa Hv (80.80%).The long terminal repeat retrotransposons (LTR-RTs), ho akarelletsa le Gypsy, Copia le unclassified RT elements, e ne e le tsona tse hlahelletseng tsa TEs, 'me 1e nkile 84.49% ea litaba tsa TE tse hlalositsoeng le 76.29 % ea genome ea Weining e kopaneng;Li-transposon tsa CACTA DNA e bile li-TE tsa bobeli tse ngata ka ho fetisisa, tse etsang 11.68% ea litaba tsa TE tse hlalositsoeng le 10.55% ea genome ea Weining e kopaneng.
Tlhahlobo ea likarolo tsa transposon tsa rye
Weining rye e ne e e-na le karolo e batlang e le holimo ea ho kenngoa ha morao tjena ea LTR-RTs le tlhōrō ea ho matlafatsa e hlahile lilemong tse limilione tse 0.5 tse fetileng (MYA), e neng e le ea morao-rao har'a mefuta e mene;tlhōrō e 'ngoe, e etsahetse hoo e ka bang 1.7 MYA, e ne e le ea khale ebile e bonoa ka harese.Boemong ba malapa a maholo, ho ile ha fumanoa ho phatloha ho hoholo ha likarolo tsa Copia ho Weining rye ho 0.3 MYA, athe li-amplification tsa Gypsy RTs li thehile haholo mokhoa oa phepelo ea li-bimodal tsa LTR-RT burst dynamics.
3. Patlisiso ea rye genome evolution le chromosome syntenies
Phapang pakeng tsa rye le koro ea diploid e etsahetse ka mor'a ho aroloa ha harese ho koro, ka linako tse fapaneng bakeng sa liketsahalo tse peli tse ka bang 9.6 le 15 MYA, ka ho latellana.1R, 2R, 3R e ne e le li-collinear ka ho feletseng le lihlopha tsa 1, 2 le 3 tsa koro, ka ho latellana.4R, 5R, 6R, 7R li ile tsa fumanoa li na le li-fusions le likarolo tse kholo.
4. Tlhahlobo ea liphatsa tsa lefutso le phello ea tsona ho liphatsa tsa lefutso tsa starch biosynthesis
Habohlokoa, lipalo tsa liphatsa tsa lefutso tse kopitsoang (TDGs) le liphatsa tsa lefutso tsa Weining rye ka bobeli li ne li le holimo ho feta tse fumanoeng bakeng sa Tu, Aet, Hv, Bd le Os.Liphatsa tsa lefutso tsa Transposed duplicated (TrDGs) le tsona li ne li le ngata ho feta tse fumanehang ka ho khetheha bakeng sa Tu le Aet.Katoloso ea liphatsa tsa lefutso tsa Rye e tsamaisana le lipalo tse phahameng tsa liphatsa tsa lefutso.Ho phatloha ho hoholo ha TE ho rye ho ka ba ho lebisitse ho palo e phahameng ea TrDGs.
Evolutionary le chromosome synteny analysis of rye genome
Tlhahlobo ea liphatlalatso tsa liphatsa tsa lefutso tsa rye le phello ea tsona ho mefuta-futa ea liphatsa tsa lefutso tse amanang le starch biosynthesis (SBRGs)
5. Karohano ea liphatsa tsa lefutso tsa protheine ea polokelo ea peo ea rye (SSP).
Li-loci tse 'ne tsa chromosomal (Sec-1 ho isa ho Sec-4) tse hlalosang li-SSP tsa rye li khethiloe ho 1R kapa 2R.Liphatsa tsa lefutso tsa α-gliadin li bile teng haufinyane tjena ka koro le mefuta e amanang haufi-ufi ka mor'a ho fapana ha koro ho tsoa ho rye.
6. Tlhahlobo ea transcription factor (TF) le liphatsa tsa lefutso tsa ho hanyetsa mafu
Tlhahlobo ea rye secalin loci
Weining rye e ne e na le mefuta e mengata ea ho hanyetsa mafu e amanang le (DRA) (1,989, Supplementary Data 3) ho feta Tu (1,621), Aet (1,758), Hv (1,508), Bd (1,178), Os (1,575), le A (1,836) ), B (1,728) le D (1,888) subgenomes tsa koro e tloaelehileng.
7. Patlisiso ea likarolo tsa liphatsa tsa lefutso tse amanang le tšobotsi ea pele ea sehlooho
Liphatsa tsa lefutso tse peli tsa FT tse nang le polelo e batlang e le holimo tlas'a maemo a letsatsi le lelelele, ScFT1 le ScFT2, li ne li hlalositsoe kopanong ea Weining genome.Masala a mabeli a amino acid a ScFT2 (S76 le T132) phosphorylation a fumanoe a amana le ho theola taolo ea nako.
Likarolo tsa nts'etsopele le liphatsa tsa lefutso tse amanang le sebopeho sa sehlooho sa Weining rye
8. Merafo ea libaka tsa chromosomal le loci tse ka 'nang tsa ameha ho rueng ha rye
Kakaretso ea li-SNP tse 123,647 li ile tsa sebelisoa ho etsa tlhahlobo e khethehileng pakeng tsa rye e lenngoeng le S. vavilovii.Lipontšo tse 11 tse khethiloeng tsa ho fiela tse khethiloeng ka index ea phokotso (DRI), fixation index (FST) le XP-CLR mokhoa.ScID1 e fumanoe e ka kenya letsoho taolong ea letsatsi la sehlooho.
Ho tsebahatsa le ho hlahloba libaka tsa chromosomal le loci tse ka 'nang tsa amana le rye domestication
Referense
Li GW le ba bang.Kopano ea boleng bo holimo ea genome e totobatsa litšobotsi tsa rye genomic le liphatsa tsa lefutso tsa bohlokoa tsa temo.Nature Genetics (2021)
Litaba le Lintlha-khōlō e ikemiselitse ho arolelana linyeoe tsa morao-rao tse atlehileng le Biomarker Technologies, ho hapa likatleho tse ncha tsa mahlale hammoho le mekhoa e tsebahalang e sebelisitsoeng nakong ea thuto.
Nako ea poso: Jan-05-2022