JUYIN HALITTA
dabi'ar halitta
Babban taro na genome yana ba da haske game da halayen hatsin rai da kuma mahimman kwayoyin halittar agronomically
PacBio |Illumina |Bionano Optical Map |Hi-C Genome Majalisar |Taswirar Halitta |Zaɓan Zaɓa |RNA-Seq |ISO-seq |SLAF-seq
Biomarker Technologies sun ba da goyon bayan fasaha akan tsarin Pacbio, Hi-C da kuma nazarin bayanai a cikin wannan binciken.
Karin bayanai
1.An samu farkon chromosomal-level high quality Rye genome, wanda yana da girman chromosome guda ɗaya ya fi 1 Gb.
2.Idan aka kwatanta da Tu, Aet da Hv genome, an lura da abubuwan da suka faru na kwanan nan na LTR-RT a cikin Rye genome, wanda ke da alhakin haɓaka girman ƙwayar hatsin rai.
3.Bambancin tsakanin hatsin rai da alkama diploid ya faru ne bayan rabuwar sha'ir da alkama, tare da lokutan bambance-bambancen abubuwan da suka faru guda biyu sun kasance kamar 9.6 da 15 MYA.
FT genes phosphorylation na iya sarrafa farkon taken a cikin hatsin rai.
4.Zaɓi share bincike yana nuna yiwuwar shigar da ScID1 a cikin ƙa'idar kwanan wata da zaɓin da zai yiwu ta hanyar gida a cikin hatsin rai.
Fage
Fage
Rye abinci ne mai kima da amfanin gona mai kiwo, muhimmin tushen kwayoyin halitta don inganta alkama da triticale, kuma abu ne mai mahimmanci don ingantaccen nazarin ilimin genomics a cikin ciyawa.Weining hatsin rai, nau'in furanni na farko da ake nomawa a kasar Sin, ya yi fice saboda tsayin daka saboda tsayin daka na juriya ga mildew powdery da tsatsa.Don fahimtar tushen kwayoyin halitta da kwayoyin halitta na dabi'un hatsin rai da kuma inganta nazarin kwayoyin halitta da kiwo a cikin hatsin rai da amfanin gona masu dangantaka, mu a nan mun tsara da kuma nazarin kwayoyin halittar Weining hatsin rai.
Nasarorin da aka samu
Rye Genome
An gina Rye genome ta hanyar haɗa karatun PacBio SMRT, taƙaitaccen jerin Illumina, da kuma waɗanda suka fito daga kamawar chromatin conformation (Hi-C), taswirar kwayoyin halitta, da bincike na BioNano.Ƙungiyoyin da aka haɗa (7.74 Gb) sun kai kashi 98.47% na ƙididdigan girman kwayoyin halitta (7.86 Gb), tare da 93.67% na contigs (7.25 Gb) da aka sanya wa chromosomes bakwai.Abubuwan maimaitawa sun ƙunshi 90.31% na kwayoyin halittar da aka haɗa.
Rye Genome
Taswirar haɗin gwiwar kwayoyin halitta (WJ) ta haɓaka ta amfani da tsire-tsire 295 F2 waɗanda aka samo daga ƙetare nau'ikan hatsin rai biyu (Weining × Jingzhou)
Hi-C taswirar tuntuɓar chromosomes bakwai da aka haɗa Weining rye (1R – 7R)
Daidaita tsakanin chromosomes bakwai da aka haɗu na Weining rye da ƙungiyoyin haɗin gwiwar hatsin rai bakwai sun haɓaka ta amfani da yawan Lo7 x Lo255 RIL.
An gano ƙimar LTR Assembly Index (LAI) na Rye genome a matsayin 18.42 da 1,393 (96.74%) na 1,440 na BUSCO da aka adana sosai. da yankunan al'adu.Jimillar kwayoyin halittar furotin 86,991, gami da 45,596 high-confidence(HC) genes da kuma 41,395 ƙananan-kwance genes (LC) an annabta.
2. Binciken TEs
Binciken TEs.Jimlar 6.99 Gb, wanda ke wakiltar 90.31% na taron Weining, an bayyana shi azaman TEs, wanda ya haɗa da abubuwa 2,671,941 na iyalai 537.Wannan abun ciki na TE ya fi wanda aka ruwaito a baya don Ta (84.70%), Tu (81.42%), Aet (84.40%), WEW (82.20%), ko Hv (80.80%).Retrotransposons mai tsayi mai tsayi (LTR-RTs), gami da Gypsy, Copia da abubuwan RT waɗanda ba a rarraba su ba, sune manyan TEs, kuma 1 sun mamaye 84.49% na bayanan bayanan TE da 76.29% na haɗe-haɗe Weining genome;CACTA DNA transposons su ne na biyu mafi yawan TEs, wanda ya ƙunshi 11.68% na bayanan bayanan TE da 10.55% na haɗe-haɗen kwayoyin halittar Weining.
Analysis na transposon abubuwa na hatsin rai
Rye na Weining yana da kaso mai tsoka na kwanan nan na shigar LTR-RT tare da kololuwar haɓakawa ya bayyana kusan shekaru miliyan 0.5 da suka gabata (MYA), wanda shine na baya-bayan nan a cikin nau'ikan nau'ikan guda huɗu;ɗayan kololuwar, ya faru kusan 1.7 MYA, ya tsufa kuma ana gani a cikin sha'ir.A matakin babban iyali, kwanan nan fashewar abubuwan Copia a cikin Rye na Weining a 0.3 MYA an samo su, yayin da haɓakar Gypsy RTs suka mamaye tsarin rarraba bimodal na LTR-RT fashe mai kuzari.
3. Binciken Rye genome evolution da chromosome syntenies
Bambanci tsakanin hatsin rai da alkama diploid ya faru ne bayan rabuwar sha'ir da alkama, tare da lokutan bambance-bambancen abubuwan biyu sun kasance kusan 9.6 da 15 MYA, bi da bi.1R, 2R, 3R sun kasance gaba ɗaya tare da ƙungiyoyi 1, 2 da 3 chromosomes na alkama, bi da bi.4R, 5R, 6R, 7R an sami akwai manyan fusions da sassa.
4. Binciken kwafin kwayoyin halitta da tasirin su akan kwayoyin halittar sitaci
Musamman ma, adadin kwayoyin halittar da aka kwafi (TDGs) da kuma kwayoyin halittar da aka kwafi (PDGs) na Weining rye duk sun fi wadanda aka samu na Tu, Aet, Hv, Bd da Os.Ƙwayoyin halittar da aka kwafi (TrDGs) su ma sun fi waɗanda aka samo musamman na Tu da Aet.Fadada kwayar halittar Rye tana tare da mafi girman lambobi na kwafin kwayoyin halitta.Ƙarar fashewar TE a cikin hatsin rai ƙila ya haifar da haɓakar adadin TrDGs.
Binciken Juyin Halitta da chromosome synteny na genome na hatsin rai
Yin nazarin kwafin kwayoyin halittar hatsin rai da tasirinsu akan nau'ikan kwayoyin halittu masu alaƙa da sitaci (SBRGs)
5. Rarraba furotin adana iri na hatsin rai (SSP) loci
An gano chromosomal loci guda huɗu (Sec-1 zuwa Sec-4) waɗanda ke ƙayyadaddun rye SSPs akan 1R ko 2R.Kwayoyin halittar α-gliadin sun samo asali ne kwanan nan a cikin alkama da nau'ikan da ke da alaƙa bayan bambancin alkama daga hatsin rai.
6. Gwajin ma'aunin rubutu (TF) da kwayoyin juriya na cututtuka
Analysis na hatsin rai secalin loci
Weining hatsin rai yana da ƙarin juriya na cututtukan da ke da alaƙa (DRA) genes (1,989, Ƙarin Bayanai 3) fiye da Tu (1,621), Aet (1,758), Hv (1,508), Bd (1,178), Os (1,575), da A (1,836). ), B (1,728) da D (1,888) subgenomes na alkama gama gari.
7. Bincika sifofin maganganun kwayoyin halittar da ke da alaƙa da sifa ta farko
Kwayoyin FT guda biyu tare da ingantacciyar magana mai girma a ƙarƙashin yanayin dogon rana, ScFT1 da ScFT2, an bayyana su a cikin taron genome na Weining.Ragowar amino acid guda biyu na ScFT2 (S76 da T132) phosphorylation an sami alaƙa tare da rage sarrafa lokaci.
Siffofin haɓakawa da maganganun kwayoyin halitta masu alaƙa da farkon taken taken Weining hatsin rai
8. Ma'adinai na chromosomal yankuna da loci yuwuwar hannu a hatsin rai domestication
An yi amfani da jimillar 123,647 SNPs don gudanar da bincike na zaɓe tsakanin hatsin rai da S.vavilovii.11 zaɓaɓɓen siginar share fage da aka gano ta hanyar raguwar index (DRI), fixation index (FST) da hanyar XP-CLR.An sami ScID1 mai yuwuwar shiga cikin ƙa'idar kwanan wata.
Ganewa da bincike na yankuna chromosomal da loci mai yuwuwar alaƙa da gidan hatsin rai
Magana
Li GW et al.Babban taro na genome yana ba da haske game da halayen hatsin rai da kuma mahimman kwayoyin halittar agronomically.Halitta Halitta (2021)
Labarai da Labarai yana da niyyar raba sabbin maganganu masu nasara tare da Biomarker Technologies, ɗaukar sabbin nasarorin kimiyya gami da fitattun dabarun da aka yi amfani da su yayin binciken.
Lokacin aikawa: Janairu-05-2022