GWASE
Sehlooho: Requencing ea genome eohle e senola tšimoloho ea Brassica napus le liphatsa tsa lefutso tse amehang ntlafatsong ea eona.
Journal: Tlhaho Puisano
NGS |WGS |Resequence |GWAS |Sengoloa |RNAseq |Brassica napus |Evolution |Ho sebetsa ka lapeng
Thutong ena, Biomarker Technologies e fane ka Litšebeletso mabapi le tatellano ea NGS, hammoho le tšehetso ea tekheniki mabapi le tlhahlobo ea bioinformatics mabapi le tatellano ea data.
Ka morao
Brassica napus(rapeseed) ke sejalo se bohlokwa sa peo ya oli le mohlala o kgabane bakeng sa ho batlisisa ditshebetso tsa polyploid speciation, evolution le kgetho.Leha ho le joalo, hore na mefuta ea liphoofolo tse hlaha kapa bafani ba malapeng e ne e le baholo-holo ba batsoali le liphatsa tsa lefutso tse tlatselitseng ho ruisoeng le ntlafatsong ea peō ea peō ha ho tsejoe.
Lisebelisoa le mekhoa
Lisebelisoa:588B. napusLiphuputso li ne li kenyelelitsoe thutong ena, ho kenyeletsoa ba 466 ba tsoang Asia, ba 102 ba tsoang Europe, ba 13 ba Amerika Leboea, le ba 7 ba Australia.Ho ipapisitsoe le lirekoto tsa tloaelo ea kholo, lisebelisoa tsena li arotsoe ka li-ecotype tse tharo;selemo (li-admissions tse 86), mariha (li-admissions tse 74), le halofo ea mariha (428 accessions).
Tatelano:Karolelano ea hoo e ka bang.5 × (ho tloha 3.37 × ho 7.71 ×)
Sequencing platform:Illumina Hiseq 4000
Tlhahiso ea lintlha:4.03 Tb data e hloekileng
SNP e letsa:BWA + GATK.Li-SNP tse 5,294,158 le li-InDels tse 1,307,151 li ile tsa fumanoa.
Liphetho
Tšimoloho ea B. napus
B. napusSubgenome e bile teng ho tsoa ho moholo-holo oa turnip ea Europe.Ketsahalo ea phallo ea liphatsa tsa lefutso ho tloha ho turnip ea Europe ho ea hoB. napus A subgenome e etsahetse ~ lilemo tse 106-1170 tse fetileng.B. napusC subgenome e kanna ea hlaha ho tsoa ho moholo-holo ea tloaelehileng oa meloko ena.Ntate-moholo oaB. napusho arohana ho tloha ho moholo-holo ea tloaelehileng oa mefuta e mene ea B. oleracea, e nang le phallo ea liphatsa tsa lefutso tsa morao-rao hoB. napus~ lilemo tse 108–898 tse fetileng.B. napusC subgenome e na le tšimoloho e rarahaneng ho feta A Subgenome.Botlolo bo matla bo hlahisitsoeng ka bobeli li-subgenomes tse peli nakong eaB. napustlhaboloho.Mariha le halofo ea marihaB. napusLi-ecotype li fapane ~ lilemong tse 60 tse fetileng, athe mariha le selemoB. napuskheloha ~ lilemo tse 416 tse fetileng, le peo ea oli le e seng ea oliB. napuskheloha ~ lilemo tse 277 tse fetileng.
Setšoantšo sa 2 Sebopeho sa baahi ba 588 B. napus accessions le 199 ea B. rapa accessions.
Setšoantšo sa 3 Sebopeho sa baahi ba 588 B. napus accessions le 119 ea B. oleracea accessions
Lipontšo tsa khetho le lithuto tsa mokhatlo oa Genome-wide.
Nakong ea mohato oa pele oa ntlafatso (FSI), mefuta e mengata ea liphatsa tsa lefutso e ile ea lahleha ho B. napus C subgenome ho feta ho A subgenome.Phapang e fokolang ea liphatsa tsa lefutso e etsahetse nakong ea FSI ho feta nakong ea mohato oa bobeli oa ntlafatso (SSI).Liphatsa tsa lefutso libakeng tsa matšoao a khetho ea SSI li matlafalitsoe ka mamello ea khatello ea maikutlo, nts'etsopele le litsela tsa metabolic.Li-loci tse 60 li amahanngoa haholo le litšobotsi tse 10 tse lebisitsoeng, ho kenyeletsoa tse 5 tse amanang le tlhahiso ea peo, bolelele ba 3 ho silique, 4 ho ea ho oli, le 48 ho boleng ba peo.
Setšoantšo sa 4 Genome-wide scanning le litlhaloso tsa libaka tse khethiloeng nakong ea SSI ea B. napus
Tlhahlobo ea Transcriptome
Lintlha tsa RNAseq tsa li-tissue tse 11 tse tsoang ho khalthiba e nang le oli e ngata le e tlase habeli le mofuta o nang le oli e tlase le mefuta e 'meli e phahameng ea mefuta e amanang le ts'ebetso ea glucosinolate biosynthetic e ne e emetsoe haholo.
Setšoantšo sa 5 Kakaretso ea taolo ea nako ea lipalesa tlas'a khetho ea ntlafatso ea ecotype ea B. napus
Puisano
Thuto ena e fane ka mohloli oa bohlokoa oa ho utloisisa tšimoloho le histori ea ntlafatso eaB. napus'me e tla thusa ho aroloa ha liphatsa tsa lefutso tsa litšobotsi tsa bohlokoa tsa agronomic complex.Li-SNP tsa bohlokoa tse amanang le mefuta e metle, matšoao a khetho le liphatsa tsa lefutso tsa mokhethoa li tla kenya letsoho haholo nakong e tlang, haholoholo ho ntlafatseng chai, boleng ba peo, litaba tsa oli, le ho ikamahanya le maemo a sejalo sena sa morao-rao sa allopolyploid le beng ka sona.
Referense
Requencing ea lefutso lohle e senola tšimoloho ea Brassica napus le liphatsa tsa lefutso tse amehang ntlafatsong ea eona[J].Tlhaho Puisano, 2019, 10(1).
Litaba le Lintlha-khōlō e ikemiselitse ho arolelana linyeoe tsa morao-rao tse atlehileng le Biomarker Technologies, ho hapa likatleho tse ncha tsa mahlale hammoho le mekhoa e tsebahalang e sebelisitsoeng nakong ea thuto.
Nako ea poso: Jan-05-2022