GWAS
Mutu: Kubwereza kwamtundu wonse kumawulula chiyambi cha Brassica napus ndi genetic loci yomwe ikukhudzidwa pakuwongolera kwake.
Magazini: Nature Communications
NGS |WGS |Zotsatira |GWASA |Chithunzi |RNASEK |Brassica napa |Evolution |Kusamalira Pakhomo
Mu kafukufukuyu, Biomarker Technologies idapereka Ntchito pakutsatizana kwa NGS, komanso chithandizo chaukadaulo pakuwunika kwa bioinformatics pakusanja deta.
Mbiri
Brassica napus(rapeseed) ndi mbewu yofunika kwambiri yambewu yamafuta komanso chitsanzo chabwino kwambiri chofufuzira njira za polyploid speciation, evolution ndi kusankha.Komabe, ngati zamoyo zakuthengo kapena opereka zoweta anali makolo a makolo ndipo majini omwe adathandizira kulera ndi kuwongolera kwa rapeseed sizikudziwikabe.
Zida ndi njira
Zida:588B. napuOphunzirawo adalowa nawo mu kafukufukuyu, kuphatikiza 466 ochokera ku Asia, 102 ochokera ku Europe, 13 ochokera ku North America, ndi 7 ochokera ku Australia.Kutengera zolemba za kukula kwa chizolowezi, zida izi zidagawidwa mumitundu itatu;masika (86 accessions), yozizira (74 accessions), ndi theka-dzinja (428 accessions).
Kutsata:Pafupifupi pafupifupi.5 × (kuyambira 3.37 × 7.71 ×)
Sequencing nsanja:Illumina Hiseq 4000
Kupanga deta:4.03 Tb deta yoyera
Kuyimba kwa SNP:BWA + GATK.5,294,158 SNPs ndi 1,307,151 InDels zinapezedwa.
Zotsatira
Chiyambi cha B. napus
B. napuSubgenome idachokera ku kholo la mpiru waku Europe.Chochitika cha gene kuchokera ku mpiru waku Europe kupita kuB. napus A subgenome idachitika ~ zaka 106-1170 zapitazo.B. napuC subgenome mwina idachokera ku kholo wamba la mibadwo iyi.Bambo waB. napukugawanika kuchokera kwa makolo wamba anayi a B. oleracea subspecies, ndi jini posachedwapa kulowaB. napu~ 108-898 zaka zapitazo.B. napuC subgenome ili ndi chiyambi chovuta kwambiri kuposa A Subgenome.Kutsekeka kwamphamvu kumayambika m'ma subgenomes awiriwaB. napuchisinthiko.M'nyengo yozizira ndi theka-dzinjaB. napuEcotypes anasiyana ~ zaka 60 zapitazo, pamene nyengo yozizira ndi masikaB. napuadasiyana ~ 416 zaka zapitazo, ndi mbewu zamafuta komanso zopanda mafutaB. napuadasiyana ~ 277 zaka zapitazo.
Chithunzi 2 Kapangidwe ka anthu a 588 B. napus accessions ndi 199 a B. rapa accessions.
Chithunzi 3 Kapangidwe ka anthu a 588 B. napus accessions ndi 119 a B. oleracea accessions
Zizindikiro zosankhidwa ndi maphunziro a Genome-wide association.
Pa gawo loyamba la kusintha (FSI), mitundu yosiyanasiyana ya majini inatayika mu subgenome ya B. napus C kusiyana ndi A subgenome.Kusiyanitsa kochepa kwa majini kunachitika panthawi ya FSI kusiyana ndi gawo lachiwiri la kusintha (SSI).Majini m'magawo osankhidwa a SSI adalimbikitsidwa pakulekerera kupsinjika, chitukuko ndi njira zama metabolic.Malo okwana 60 okhudzana kwambiri ndi makhalidwe 10, kuphatikizapo 5 okhudzana ndi zokolola zambewu, 3 mpaka silique kutalika, 4 ku mafuta okhutira, ndi 48 ku khalidwe lambewu adadziwika.
Chithunzi cha 4 Genome-wide scanning ndi zofotokozera za zigawo zosankhidwa panthawi ya SSI ya B. napus
Kusanthula kwa Transcriptome
Deta ya RNAseq ya minyewa ya 11 yochokera kumafuta ochuluka kwambiri komanso otsika kawiri komanso mafuta ocheperako komanso mitundu iwiri yamitundu yodziwika bwino yokhudzana ndi njira ya glucosinolate biosynthetic idaimiridwa kwambiri.
Chithunzi 5 Chidule cha malamulo a nthawi ya maluwa posankha kusintha kwachilengedwe kwa B. napus
Zokambirana
Kafukufukuyu adapereka chida chofunikira kumvetsetsa chiyambi ndi mbiri yabwino yaB. napundipo zithandizira kugawanika kwa ma genetic maziko ofunikira agronomic zovuta.Ma SNP ofunikira okhudzana ndi mitundu yabwino, ma siginecha osankhidwa ndi majini osankhidwa azithandizira kwambiri mtsogolo, makamaka pakuwongolera zokolola, mtundu wambewu, kuchuluka kwamafuta, komanso kusinthika kwa mbewu yaposachedwa ya allopolyploid ndi abale ake.
Buku
Kuphatikizika kwa ma genome athunthu kumawonetsa chiyambi cha Brassica napus ndi genetic loci yomwe ikukhudzidwa ndikusintha kwake[J].Nature Communications, 2019, 10(1).
Nkhani ndi Mfundo Zazikulu ikufuna kugawana milandu yaposachedwa kwambiri ndi Biomarker Technologies, kujambula zomwe zakwaniritsa zasayansi komanso njira zodziwika bwino zomwe zimagwiritsidwa ntchito pophunzira.
Nthawi yotumiza: Jan-05-2022