I-GWAS
Isihloko: Ukulandelelana kwe-genome yonke kubonisa imvelaphi ye-Brassica napus kunye ne-loci yofuzo ebandakanyekayo ekuphuculeni kwayo.
Ijenali: Unxibelelwano lweNdalo
NGS |WGS |Ilandelelanisa |GWAS |Umbhalo obhaliweyo |RNAseq |Brassica napus |Indaleko |Ukwenziwa kwasekhaya
Kolu phononongo, i-Biomarker Technologies ibonelele ngeeNkonzo ngokulandelelana kwe-NGS, kunye nenkxaso yobugcisa kuhlalutyo lwe-bioinformatics kwidatha yokulandelelana.
Imvelaphi
Brassica napus(i-rapeseed) sisityalo esibalulekileyo sembewu ye-oyile kunye nomzekelo ogqwesileyo wokuphanda iinkqubo ze-polyploid speciation, i-evolution kunye nokukhetha.Nangona kunjalo, ukuba izilwanyana zasendle okanye abaxhasi basekhaya yayingookhokho babazali kwaye imizila yemfuza enegalelo ekufuyweni nasekuphuculweni kwembewu yokudlwengulwa akwaziwa.
Impahla nenkqubo
Izixhobo:588B. napusUkufikelela kwakubandakanyeka kolu phononongo, kubandakanywa i-466 evela e-Asia, i-102 evela eYurophu, i-13 evela kuMntla Melika, kunye ne-7 evela e-Australia.Ngokusekwe kwiirekhodi zesiqhelo sokukhula, ezi mathiriyeli zahlulwa zaba zii-ecotypes ezintathu;intwasahlobo (86 accessions), ebusika (74 accessions), kunye nesiqingatha sebusika (428 accessions).
Ulandelelwano:I-avareji malunga.5× (ukusuka kwi-3.37× ukuya kwi-7.71×)
Iqonga lolandelelwano:Illumina Hiseq 4000
Ukuveliswa kwedatha:4.03 Tb idatha ecocekileyo
SNP umnxeba:BWA + GATK.I-5,294,158 ye-SNP kunye ne-1,307,151 ye-InDels yafunyanwa.
Iziphumo
Imvelaphi ka-B. napus
B. napusI-subgenome yavela kukhokho wetheniphu yaseYurophu.Isiganeko sokuhamba kofuzo ukusuka kwitheniphu yaseYurophu ukuya kwiB. napus I-subgenome yenzeka ~106–1170 kwiminyaka eyadlulayo.B. napusI-C subgenome inokuba yavela kwisinyanya esiqhelekileyo sale migca.Ukhokho weB. napusukwahlukana ukusuka kukhokho oqhelekileyo ezine B. oleracea subspecies, kunye gene flow yakutshanje kwiB. napus~Iminyaka eyi-108–898 eyadlulayo.B. napusI-subgenome ye-C inemvelaphi entsonkothileyo kune-A Subgenome.I-bottleneck eyomeleleyo iphuhliswe kuzo zombini ii-subgenomes ezimbini ngexeshaB. napusindaleko.Ebusika nakwisiqingatha sebusikaB. napusIi-ecotypes zahlukana ~ kwiminyaka engama-60 eyadlulayo, ngelixa ebusika nasentwasahloboB. napusyahlukana ~ 416 kwiminyaka eyadlulayo, kunye ne-oyile kunye ne-non-oilseedB. napusyahlukana ~ 277 kwiminyaka eyadlulayo.
Isazobe 2 Ulwakhiwo lwabantu abangama-588 B. ukungena kwinapus kunye ne-199 ye-B. rapa accessions.
Isazobe 3 Ubume boluntu lwe-588 B. ukungena kwi-napus kunye ne-119 ye-B. oleracea accessions
Imiqondiso yokukhetha kunye nezifundo zombutho obanzi weGenome.
Ngexesha lenqanaba lokuqala lokuphucula (FSI), ukuhlukahluka kofuzo kwalahleka kwi-subgenome ye-B. napus C kune-A subgenome.Ukwahlulwa kofuzo okuncinci kwenzeka ngexesha le-FSI kunenqanaba lesibini lokuphucula (SSI).I-Gene kwiingingqi ze-SSI-zokukhethwa kweempawu ziye zatyetyiswa ekunyamezeleni uxinzelelo, uphuhliso kunye neendlela ze-metabolic.Ii-loci ezingama-60 zinxulunyaniswa kakhulu neempawu ezili-10 ekujoliswe kuzo, kubandakanywa i-5 enxulumene nesivuno sembewu, i-3 ukuya kubude be-silique, i-4 kumxholo we-oyile, kunye ne-48 kumgangatho wembewu ichongiwe.
Umzobo we-4 ukuskena ngokubanzi kweGenome kunye neenkcazo zemimandla ekhethiweyo ngexesha le-SSI ye-B. napus
Uhlalutyo lwe-Transcriptome
Idatha ye-RNAseq yezicubu ze-11 ezivela kwi-oyile ephezulu kunye ne-double-low cultivar kunye ne-oyile ephantsi-i-oyile kunye ne-double-high-high cultivar ezichongiweyo zofuzo ezinxulumene nenkqubo ye-glucosinolate biosynthetic zazibonakaliswe kakhulu.
Umfanekiso wesi-5 Ushwankathelo lolawulo lwexesha leentyantyambo phantsi kokhetho lophuculo lwe-ecotype ye-B. napus
Ingxoxo
Olu phononongo lubonelele ngomthombo oxabisekileyo wokuqonda imvelaphi kunye nembali yophuculo lweB. napuskwaye iya kuququzelela ukuchithwa kweziseko zofuzo zeempawu ezibalulekileyo ze-agronomic complex.Ii-SNP ezibalulekileyo ezinxulunyaniswa nokwahluka okuncomekayo, imiqondiso yokukhetha kunye nofuzo lomgqatswa ziya kuba negalelo kakhulu kwixesha elizayo, ngakumbi ekuphuculeni isivuno, umgangatho wembewu, umxholo we-oyile, kunye nokuqhelana nesi sityalo samva nje se-allopolyploid kunye nezalamane zaso.
Isalathiso
Ulandelelwano lwe-genome yonke luveza imvelaphi ye-Brassica napus kunye ne-loci yofuzo ebandakanyekayo ekuphuculeni kwayo[J].Unxibelelwano lweNdalo, 2019, 10(1).
Iindaba neembalasane ijolise ekwabelaneni ngamatyala aphumeleleyo amva nje kunye neBiomarker Technologies, ukubamba impumelelo yesayensi enoveli kunye nobuchule obubalaseleyo obusetyenzisiweyo ngexesha lokufunda.
Ixesha lokuposa: Jan-05-2022