PHEPO PHETOHO
PNAS
Tšimoloho ea ho iphetola ha lintho le nalane ea lehae ea tlhapi ea khauta (Carassius auratus)
PacBio |Ilumina |'Mapa oa Bionano Genome |Kopano ea Hi-C Genome |'Mapa oa liphatsa tsa lefutso |GWAS |RNA-Seq
Lintlha-khōlō
1.Goldfish genome e ile ea nchafatsoa ka mofuta oa kopano ea boleng bo holimo, e tiisang 95.75% ea li-contigs ho li-pseudochromosomes tse 50 (Scaffold N50=31.84 Mb).Li-subgenome tse peli li ile tsa aroloa.
2. Likarolo tsa liphatsa tsa lefutso tse khethiloeng ka nako ea lehae li ile tsa khetholloa ho tsoa ho data ea batho ba 201, ho hlahisa mefuta e fetang 390 ea liphatsa tsa lefutso tse amanang le mekhoa ea lehae.
3.GWAS ho dorsal fin ho goldfish e ruuoang lapeng e senotse liphatsa tsa lefutso tse 378 tse amanang le tsona.Motlalehi oa tyrosine-protein kinase o ile a khetholloa e le mofuta oa mokhethoa o amanang le pepeneneng.
Ka morao
Goldfish (Carassius auratus) ke e 'ngoe ea litlhapi tsa bohlokoa ka ho fetisisa tse ruuoang, tse neng li ruuoa ho tloha crucian carp Chaena ea boholo-holo.Ba hlalositsoe ke Charles Darwin e le "Ho feta mefuta e mengata e sa tšoaneng ea mebala, re kopana le liphetoho tse sa tloaelehang tsa sebopeho".Likarolo tse fapaneng haholo le nalane e telele ea ho ruisa le ho ikatisa li etsa hore tlhapi ea khauta e be mofuta o motle oa liphatsa tsa lefutso bakeng sa fisioloji ea litlhapi le thuto ea ho iphetola ha lintho.
Katleho
Goldfish genome
Jtlhahlobo ea mafura ea data ea ho latellana ha PacBio le Illumina e fana ka kopano ea pele ea 1.657 G (Contig N50 = 474 KB).'Mapa oa optical oa Bionano o ile oa hlahisoa 'me oa lokisa kopano hore e be 1.73 Gb ka boholo (Hakanyo ea boholo ba genome: 1.8 Gb).Kopano e thehiloeng ho Hi-C e ntlafalitse le ho feta scaffold N50 ho tloha 606 Kb ho isa 31.84 Mb mme e fihletse 95.75% (1.65 Gb) e shebaneng le sekhahla se laetsoeng sa ho emisa.Genome e na le liphatsa tsa lefutso tse 56,251 le liphatlalatso tse telele tse 10,098 tse sa khoutang.Ho feta moo, libaka tse 38 tse ka bang teng tsa centromeric li ile tsa boleloa esale pele ho li-chromosome tse 50.
Fig.1 Gold fish Genome
Tho ile ha hlokomeloa lihlopha tse hlakileng tsa li-subgenome ho li-chromosome tse 50 tsa goldfish tse bakiloeng ke ketsahalo ea khale ea ho tsoakanngoa.Sehlopha sa li-chromosome tse nang le karolo e phahameng ea lipalo tse hokahantsoeng lipakeng tsa goldfish le Barbinae li hlalositsoe e le subgenome A (ChrA01~A25), ke hore subgenome e tloaelehileng ho Barbinae, le tse setseng e le subgenome B (ChrB01~B25).
Ho fiela ka lapeng le ho khetha
Akakaretso ea mefuta e 16 ea mofuta o hlaha oa crucian carps le mefuta e 185 e emelang goldfish e bile tatelano ka botebo ba tatellano e ka bang 12.5X, e hlahisang 4.3 terabases ea data.Tlhahlobo ea Phylogenetic le PCA e netefalitse kamano e haufi pakeng tsa tlhapi e tloaelehileng ea khauta le crucian carp ho feta litlhapi tse ling tsa khauta, tse ileng tsa arohana ka meloko e 'meli.
LD tshekatsheko ya ho bola ha batho ba ka hodimo ho tse nne e tsheheditse boteng ba diphatsa tsa lefutso tsa batho nakong ya ho ruwa le kgetho e matla ya maiketsetso ho goldfish.Keketseho ea liphatsa tsa lefutso (π) ho tloha ho crucian carp ho ea ho tlhapi e tloaelehileng ea goldfish ho ea pele ho Wen goldfish le Egg goldfish e bontšitse pokello e kholo ea liphatsa tsa lefutso nakong ea ho ruuoa ha tsona.Libaka tse 50 tse ikhethileng tsa genomic tse koahelang 25.2 Mb le liphatsa tsa lefutso tse 946 li ile tsa khetholloa ho tsoa boitsebisong ba baemeli (33 goldfish le 16 crucian crap).Ho atolosoa ha tlhahlobo ho batho ba 201, liphatsa tsa lefutso tse 393 li bonts'itse libaka tsa ho fiela ho phethehileng.Liphatsa tsa lefutso tsena li ile tsa fumanoa tsa mefuta-futa e fokolang, e ka 'nang eaba e kentse letsoho ho li-phenotypes tse amanang le litšobotsi tse kholo tsa malapeng ho goldfish.
Setšoantšo sa 3 Genome-wide domestication-associated analysis
GWAS ho tlhapi ea khauta e ruuoang lapeng
Dorsal fin ke tšobotsi ea bohlokoa e khethollang Wen goldfish ho Egg goldfish.GWAS ea dorsal fin ho 96 Wen goldfish le 87 Egg goldfish e senotse liphatsa tsa lefutso tse 378 tse phatlalalitsoeng ho li-chromosome tse 13 'me ho ile ha hlokomeloa kabo e sa leka-lekaneng ea liphatsa tsena tsa lefutso lipakeng tsa subgenome.Tlhahlobo ea ts'ebetso ho liphatsa tsa lefutso tsa mokhethoa e totobalitse lits'ebetso tsa baeloji ho kenyelletsa "Cell surface receptor signing", "transmembrane transportation", "Skeletal system development", jj.
Fig.4 GWAS ea dorsal fin holim'a tlhapi ea khauta e ruuoang lapeng
In GWAS ea litšobotsi tse amanang le sekala se pepeneneng, tlhōrō e le 'ngoe e matla ea mokhatlo e ile ea fumanoa.Mofuta o kentseng seamoheli sa tyrosine-protein kinase se fumanoe sebakeng se seng sa bakhethoa.
Setšoantšo sa 5 GWAS ea litšobotsi tse amanang le sekala se pepeneneng
Referense
Chen D le al.Tšimoloho ea ho iphetola ha lintho le nalane ea lehae ea tlhapi ea khauta (Carassius auratus).PNAS (2020)
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Nako ea poso: Jan-04-2022