KUSINTHA KWA GENOME
PNAS
Chisinthiko ndi mbiri yoweta nsomba zagolide (Carassius auratus)
PacBio |Ilumina |Mapu a Bionano Genome |Hi-C Genome Assembly |Genetic Mapu |GWASA |RNA-Seq
Mfundo zazikuluzikulu
1.Goldfish genome idasinthidwa ndi mtundu wapamwamba kwambiri, wokhazikika 95.75% ya contigs mu 50 pseudochromosomes (Scaffold N50=31.84 Mb).Ma subgenomes awiri adalumikizidwa.
2. Madera omwe amaseseredwa mwachisawawa panthawi yoweta adadziwika kuchokera kuzinthu zofananira za anthu 201, akuwonetsa mitundu yopitilira 390 yomwe ingakhale yokhudzana ndi zoweta.
3.GWAS pa dorsal fin mu goldfish yoweta inavumbulutsa majini 378 omwe amagwirizana nawo.Mtolankhani wa tyrosine-protein kinase adadziwika ngati jini yochititsa chidwi yokhudzana ndi kuwonekera.
Mbiri
Goldfish ( Carassius auratus ) ndi imodzi mwa nsomba zofunika kwambiri zoweta, zomwe zinkaweta kuchokera ku crucian carp ku China.Adanenedwa ndi Charles Darwin kuti "Podutsa mitundu ingapo yamitundu yosiyanasiyana, timakumana ndi zosinthika modabwitsa kwambiri".Kusiyanasiyana kosiyanasiyana komanso mbiri yakale yoweta ndi kuswana kumapangitsa nsomba za goldfish kukhala njira yabwino kwambiri yopangira chibadwa cha nsomba ndi chisinthiko.
Zopambana
Goldfish genome
JKusanthula kwamafuta kwa PacBio ndi Illumina kutsata zotsatizana zapawiri kumapereka msonkhano woyamba wa 1.657 G (Contig N50=474 KB).Mapu owoneka bwino a Bionano adapangidwa ndikuwongolera gululo kukhala 1.73 Gb kukula kwake (Kuyerekeza kukula kwa genome: 1.8 Gb).Msonkhano wokhazikitsidwa ndi Hi-C udapititsa patsogolo scaffold N50 kuchokera ku 606 KB mpaka 31.84 Mb ndipo idapeza 95.75% (1.65 Gb) yokhazikika ndikuyitanitsa kukhazikika.Ma genome amakhala ndi ma gene 56,251 okhota komanso 10,098 osalemba zilembo zazitali.Kuphatikiza apo, madera 38 omwe angakhale apakati adanenedweratu mwa ma chromosome 50.
Fig.1 Goldfish Genome
TMagulu omveka bwino a ma subgenomes adadziwika mu ma chromosomes 50 a goldfish omwe adachokera ku chochitika chakale cha kusakanizidwa.Seti ya ma chromosome okhala ndi gawo lalikulu la zowerengera zolumikizidwa pakati pa goldfish ndi Barbinae adafotokozedwa kuti subgenome A (ChrA01~A25), mwachitsanzo, subgenome yodziwika bwino kwa Barbinae, ndi zotsalira monga subgenome B (ChrB01~B25).
Kusesa kwapakhomo ndi kusankha
ANsomba zokwana 16 zakuthengo za crucian carps ndi mitundu 185 ya nsomba za golide zinali zotsatizana ndi kuya kwa pafupifupi 12.5X, zomwe zinapanga ma data 4.3.Kukonzanso kwa Phylogenetic ndi kusanthula kwa PCA kunatsimikizira kugwirizana kwapafupi pakati pa nsomba za golide ndi crucian carp kusiyana ndi nsomba zina za golide, zomwe zinagawanika kukhala mizere iwiri.
LKufufuza kwa D kuwola pamagulu anayi omwe ali pamwambawa kunatsimikizira kukhalapo kwa chiwerengero cha chibadwa cha anthu panthawi yoweta komanso kusankha kwakukulu kochita kupanga mu goldfish.Kuchulukitsa kwamitundumitundu (π) kuchokera ku crucian carp kupita ku nsomba zagolide wamba kupita ku Wen goldfish ndi Egg goldfish kunawonetsa kuchuluka kwakukulu kwamitundu yosiyanasiyana panthawi yoweta.Madera 50 osankhidwa akusesa ma genomic okhala ndi 25.2 Mb ndi 946 majini adadziwika kuchokera ku data yoyimira (33 goldfish ndi 16 crucian crap).Kukulitsa kusanthula kwa anthu 201, majini 393 adawonetsa madera omwe adamaliza kusankha.Ma jini awa adapezeka kuti ali ndi mitundu yotsika, yomwe mwina idathandizira ku ma phenotypes omwe amakhudzana ndi zoweta zazikulu za nsomba za golide.
Fig.3 Genome-wide domestication-associated analysis
GWAS pa nsomba za golide zoweta
Dorsal fin ndi chinthu chofunikira chomwe chimasiyanitsa Wen goldfish ndi Egg goldfish.GWAS ya dorsal fin pa 96 Wen goldfish ndi 87 Egg goldfish inavumbulutsa majini okwana 378 omwe amafalikira mu ma chromosomes 13 ndipo kugawidwa kosiyana kwa majiniwa pakati pa subgenomes kunawonedwa.Kusanthula kogwira ntchito pamajini osankhidwawa kunawunikira njira zachilengedwe kuphatikiza "Cell surface receptor signing", "Transmembrane transportation", "Skeletal system development", ndi zina zambiri.
Chithunzi cha 4 GWAS cha dorsal fin pa nsomba za golide zoweta
In GWAS ya mawonekedwe okhudzana ndi sikelo yowonekera, chiwongola dzanja chimodzi cholimba chidapezeka.Jini yomwe imasunga cholandilira cha tyrosine-protein kinase idazindikirika m'chigawo chimodzi cha ofuna kusankha.
Fig.5 GWAS ya mawonekedwe okhudzana ndi sikelo
Buku
Chen D et al.Chisinthiko ndi mbiri yoweta nsomba zagolide (Carassius auratus).PNAS (2020)
Nkhani ikufuna kugawana milandu yaposachedwa kwambiri ndi Biomarker Technologies, kujambula zomwe zakwaniritsa zasayansi komanso njira zodziwika bwino zomwe zimagwiritsidwa ntchito pophunzira.
Nthawi yotumiza: Jan-04-2022