GENOME EVOLUTION
PNAS
Inkomoko y'ubwihindurize n'amateka yo gutunga amafi ya zahabu (Carassius auratus)
PacBio |Illumina |Ikarita ya Bionano Ikarita |Muraho-C Inteko rusange |Ikarita ya genetike |GWAS |RNA-Seq
Ingingo z'ingenzi
1.Indabyo za Goldfish zavuguruwe hamwe na verisiyo yo guterana yo mu rwego rwohejuru, ihuza 95,75% ya contigs muri 50 pseudochromosomes (Scaffold N50 = 31.84 Mb).Subgenomes ebyiri zaciwe.
2.Uturere twose two gutoranya abantu mugihe cyo gutura mu rugo byagaragaye uhereye kumibare yabantu 201, bagaragaza genes zabakandida barenga 390 zishobora kuba zifitanye isano nimico yo murugo.
3.GWAS kuri dorsal fin mumafi yororerwa mu rugo yerekanye genes 378 zabakandida zishobora kuba zifitanye isano.Umunyamakuru wa tyrosine-protein kinase yamenyekanye nkumukandida utera gene ifitanye isano no gukorera mu mucyo
Amavu n'amavuko
Goldfish (Carassius auratus) ni rimwe mu mafi yororerwa cyane, yororerwa muri karipi yabambwe mu Bushinwa bwa kera.Bavuzwe na Charles Darwin ngo "Tunyuze hejuru y'amabara atandukanye atagira ingano, duhura no guhindura imiterere idasanzwe".Ibintu bitandukanye cyane n'amateka maremare yo gutunga no korora bituma amafi ya zahabu ari uburyo bwiza bwo kwerekana imiterere ya fiologiya na evolution.
Ibyagezweho
Goldfish genome
Jisesengura ryamavuta ya PacBio na Illumina ikurikirana-ikurikirana itanga umusaruro wambere 1.657 G umushinga winteko (Contig N50 = 474 Kb).Ikarita ya optique ya Bionano yarakozwe kandi ikosora inteko muri 1.73 Gb (Ingano ya genome igereranijwe: 1.8 Gb).Inteko ishingiye kuri Hi-C yarushijeho kunoza scafold N50 kuva 606 Kb igera kuri 31.84 Mb kandi igera kuri 95,75% (1.65 Gb) yerekanwe kandi itegeka igipimo cya ankoring.Ikirangantego kigizwe na 56,251 coding ya coding na 10.098 z'uburebure butari code.Byongeye kandi, uturere 38 dushobora kuba hagati ya chromosomes.
Igishushanyo.1 Ifi Zahabu Genome
Two wasobanutse neza ya subgenomes yamenyekanye muri chromosomes 50 ya zahabu yavuye mubikorwa bya kera byo kuvanga.Igice cya chromosomes hamwe nigice kinini cyibisomwa bihujwe hagati yamafi ya zahabu na Barbinae byasobanuwe nka subgenome A (ChrA01 ~ A25), ni ukuvuga subgenome ihuriweho na Barbinae, naho ibisigaye nka subgenome B (ChrB01 ~ B25).
Gutunga urugo no gutoranya ibintu
Ayose hamwe 16 yibiti byo mumashyamba hamwe na 185 zihagarariye amafi ya zahabu byari bihuye hamwe nuburinganire bwikigereranyo cya 12.5X, bikabyara terabase 4.3.Kwiyubaka kwa Phylogenetike hamwe nisesengura rya PCA byemeje isano iri hagati y amafi asanzwe ya zahabu na karipi ya karusiya kurusha andi mafi ya zahabu, agabanijwemo imirongo ibiri.
LD isesengura ryangirika hejuru ya subpopulations zashyigikiye ko habaho umuturage wabaturage mugihe cyo gutunga no gutoranya ibihangano muri zahabu.Kongera ubwoko butandukanye (π) kuva kuri karipi ya karusiya kugeza kumafi ya zahabu kugeza kuri Wen goldfish na Egg zahabu byerekana ko habaho ihindagurika ryinshi ryimiterere yabantu mugihe babaga.Uturere 50 twatoranijwe dukwirakwiza 25.2 Mb na 946 genes zagaragaye mubisobanuro byerekana (amafi 33 ya zahabu na 16 ya crapi).Kwagura isesengura kubantu 201, gen 393 zerekanye uturere twarangije gutoranya.Izi genes zabonetse zifite ubudasa butandukanye, zishobora kuba zigira uruhare muri fenotipike ijyanye nimiterere yo gutunga amafi muri zahabu.
Igishushanyo.3 Genome-yagutse yo mu rugo ifitanye isano nisesengura
GWAS kumafi ya zahabu
Dorsal fin nikintu cyingenzi gitandukanya Wen zahabu na Egg zahabu.GWAS ya dorsal fin kuri 96 Wen goldfish na 87 Egg zahabu yerekana amagi 378 yabakandida yakwirakwijwe muri chromosomes 13 kandi ikwirakwizwa rya genes hagati ya subgenomes ryaragaragaye.Isesengura ry'imikorere kuri genes z'abakandida ryagaragaje inzira y'ibinyabuzima harimo "Ikimenyetso cyo hejuru cya selile yerekana ibimenyetso", "Transmembrane transport", "Iterambere rya Skeletal", nibindi.
Igishushanyo.4 GWAS ya fin ya dorsal kumafi ya zahabu
In GWAS yimiterere yibipimo bifitanye isano, impinga imwe ikomeye ikomeye yagaragaye.Gene igizwe na tyrosine-protein kinase reseptor yamenyekanye muri kamwe mu turere tw’abakandida.
Igicapo.5 GWAS yimiterere igaragara neza
Reba
Cinkoko D n'abandi.Inkomoko y'ubwihindurize n'amateka yo gutunga amafi ya zahabu (Carassius auratus).PNAS (2020)
Amakuru igamije gusangira ibibazo byatsinzwe na Biomarker Technologies, gufata ibyagezweho mu buhanga hamwe nubuhanga bukomeye bwakoreshejwe mugihe cyo kwiga.
Igihe cyo kohereza: Mutarama-04-2022