Metagenomics (NGS)

Shotgun metagenomics tare da Illumina sanannen kayan aiki ne don nazarin microbiomes ta hanyar tsara DNA kai tsaye daga samfuran hadaddun, yana ba da damar nazarin duka nau'ikan taxonomic da bambancin aiki. Bututun BMKCloud metagenomic (NGS) yana farawa tare da kulawa mai inganci da haɗuwa na metagenome, daga inda ake hasashen kwayoyin halitta kuma ana tattara su cikin bayanan da ba su da yawa waɗanda aka keɓance don aiki da haraji ta amfani da bayanai masu yawa. Ana amfani da wannan bayanin don bincika tsakanin-samfurin bambance-bambancen harajin haraji (bambancin alpha) da tsakanin-samfurin bambance-bambancen (banbancin beta). Binciken bambance-bambance tsakanin ƙungiyoyi yana samo OTUs da ayyukan nazarin halittu waɗanda suka bambanta tsakanin ƙungiyoyin biyu ta yin amfani da gwaje-gwajen parametric da marasa daidaituwa, yayin da nazarin daidaituwa ya danganta waɗannan bambance-bambance ga abubuwan muhalli.