Juyin Halitta
Amfanin Sabis
Takagi et al.,Jaridar shuka, 2013
● Ƙimar bambancin lokaci da saurin jinsuna dangane da bambancin matakin nucleotide da amino acid.
● Bayyana mafi dangantakar da aka dogara da Phylogenetic tsakanin jinsi tare da rage yawan tasirin juyin halitta da kuma juyin halitta na layi daya
● Ƙirƙirar haɗin kai tsakanin canje-canjen kwayoyin halitta da nau'in halitta don gano kwayoyin halitta masu alaƙa
● Ƙimar bambance-bambancen kwayoyin halitta, wanda ke nuna yuwuwar juyin halitta
● Saurin juyowa lokaci
● Ƙwarewa mai yawa: BMK ya tara kwarewa mai yawa a cikin yawan jama'a da ayyukan da suka shafi juyin halitta fiye da shekaru 12, wanda ya shafi daruruwan nau'o'in nau'in, da dai sauransu kuma ya ba da gudummawa a cikin manyan ayyuka sama da 80 da aka buga a Nature Communications, Molecular Plants, Plant Biotechnology Journal, da dai sauransu.
Ƙayyadaddun Sabis
Kayayyaki:
A al'ada, ana ba da shawarar aƙalla ƙananan jama'a uku (misali nau'i ko nau'i).Kowane yanki ya kamata ya ƙunshi mutane ƙasa da 10 (Tsarin 15, ana iya rage shi don nau'ikan da ba kasafai ba).
Dabarun tsarawa:
* Ana iya amfani da WGS don nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan suna amfani da su, yayin da SLAF-Seq ya dace da nau'ikan ko dai tare da ko ba tare da ma'anar genome ba, ko ma'anar kwayar halitta mara kyau.
| Aiwatar da girman genome | WGS | SLAF-Tags (×10,000) |
| ≤ 500 Mb | 10 × / mutum | WGS an fi ba da shawarar |
| 500 Mb - 1 Gb | 10 | |
| 1 Gb - 2 Gb | 20 | |
| ≥2 GB | 30 |
Binciken bioinformatics
● Binciken juyin halitta
● Zaɓar zaɓe
● kwararar kwayoyin halitta
● Tarihin alƙaluma
● Lokacin rarrabuwa
Samfuran Bukatun da Bayarwa
Samfuran Bukatun:
| Nau'o'i | Nama | Farashin WGS-NGS | SLAF |
| Dabba
| Visceral nama |
0.5-1 g
|
0.5g ku
|
| Naman tsoka | |||
| Jinin dabbobi masu shayarwa | 1.5ml
| 1.5ml
| |
| Jinin kaji/Kifi | |||
| Shuka
| Fresh Leaf | 1 ~ 2g | 0.5-1 g |
| Petal/Stem | |||
| Tushen/Iri | |||
| Kwayoyin halitta | Tantanin halitta |
| gDNA | Hankali | Adadin (ug) | Saukewa: OD260/OD280 |
| SLAF | ≥35 | ≥1.6 | 1.6-2.5 |
| Farashin WGS-NGS | ≥1 | ≥0.1 | - |
Gudun Aikin Sabis
Gwajin ƙira
Samfurin bayarwa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
*Sakamakon Demo da aka nuna anan duk sun fito ne daga kwayoyin halittar da aka buga tare da BMKGENE
1.Binciken Juyin Halitta ya ƙunshi gina bishiyar phylogenetic, tsarin yawan jama'a da PCA dangane da bambancin kwayoyin halitta.
Bishiyar phylogenetic tana wakiltar alaƙar taxonomic da juyin halitta tsakanin nau'ikan da ke da kakanni ɗaya.
PCA na nufin ganin kusanci tsakanin ƙananan jama'a.
Tsarin yawan jama'a yana nuna kasancewar ɓangarorin ɓangarorin jinsin halittu dangane da mitocin allele.
Chen, da.al.,PNAS, 2020
2.Zaɓaɓɓen shara
Zaɓan zaɓi yana nufin tsari wanda aka zaɓi wuri mai fa'ida kuma ana ƙara mitoci na rukunin yanar gizo masu tsaka-tsaki da kuma raguwar wuraren da ba su da alaƙa, yana haifar da raguwar yanki.
Gano-fadi na genome akan zaɓaɓɓun yankuna ana sarrafa su ta hanyar ƙididdige fihirisar yawan jama'a (π, Fst, Tajima's D) na duk SNPs a cikin taga mai zamewa (100 Kb) a wani mataki (10 Kb).
Bambancin Nucleotide (π)
Tajima's D
Fihirisar gyarawa (Fst)
Wu, da.al.,Shuka Kwayoyin Halitta, 2018
3.Gene yawo
Wu, da.al.,Shuka Kwayoyin Halitta, 2018
4. Tarihin alƙaluma
Zhang, da.al.,Halin Halitta & Juyin Halitta, 2021
5.Lokacin rarrabuwa
Zhang, da.al.,Halin Halitta & Juyin Halitta, 2021
BMK Case
Taswirar bambance-bambancen kwayoyin halitta yana ba da haske game da tushen kwayoyin halittar Kabejin Sinawa na bazara (Brassica rapa ssp. Pekinensis) zaɓi.
Buga: Shuka Kwayoyin Halitta, 2018
Dabarun tsarawa:
Maimaitawa: zurfin jeri: 10×
Sakamako mai mahimmanci
A cikin wannan binciken, an sarrafa kabeji 194 na kasar Sin don sake tsarawa tare da matsakaicin zurfin 10 ×, wanda ya samar da SNPs 1,208,499 da InDels 416,070.Binciken phylogenetic akan waɗannan layukan 194 ya nuna cewa ana iya raba waɗannan layin zuwa nau'ikan halittu guda uku, bazara, bazara da kaka.Bugu da ƙari, tsarin yawan jama'a da bincike na PCA sun nuna cewa kabeji na kasar Sin na bazara ya samo asali ne daga wani kabeji na kaka a Shandong, kasar Sin.Daga baya an gabatar da waɗannan zuwa Koriya da Japan, an ketare su tare da layin gida kuma an gabatar da wasu nau'ikan su a ƙarshen zamani zuwa China kuma a ƙarshe sun zama kabeji na Sin na bazara.
Binciken da aka yi kan nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau’in nau’in nau’in nau’in nau’in nau’in nau’in halitta, ya nuna nau’in nau’in nau’in nau’in nau’in halitta guda 23 da aka zaba, wadanda biyu daga cikinsu sun cika da yankin sarrafa lokaci bisa taswirar QTL.An gano waɗannan yankuna biyu suna ɗauke da mahimman kwayoyin halittar da ke daidaita furanni, BrVIN3.1 da BrFLC1.Wadannan kwayoyin halitta guda biyu an kara tabbatar da cewa suna da hannu a lokacin bolting ta hanyar binciken kwafi da gwaje-gwajen transgenic.
 Binciken tsarin yawan jama'a akan cabbages na kasar Sin |  Bayanan kwayoyin halitta akan zabin kabeji na kasar Sin |
Tongbing, et al."Taswirar Bambancin Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittu tana Ba da Hazaka ga Tushen Halitta na Kabeji na Sinanci (Brassica rapa ssp.pekinensis) Zaɓi."Tsiren kwayoyin halitta,11 (2018): 1360-1376.
