Babban bincike na Segregant
Amfanin Sabis
Takagi et al., Jaridar shuka, 2013
● Daidaitaccen wuri: Haɗuwa da yawa tare da 30 + 30 zuwa 200 + 200 mutane don rage girman amo;Hasashen yanki na ɗan takarar mutatant wanda ba daidai ba.
● Cikakken bincike: Bayanin aikin ɗan takara mai zurfi, ciki har da NR, SwissProt, GO, KEGG, COG, KOG, da dai sauransu.
● Saurin Juyin Juyawa: Gaggawar gano kwayoyin halitta a cikin kwanakin aiki 45.
● Ƙwarewa mai zurfi: BMK ya ba da gudummawa a cikin dubban halaye na gida, yana rufe nau'o'in nau'i daban-daban kamar amfanin gona, kayayyakin ruwa, gandun daji, furanni, 'ya'yan itatuwa, da dai sauransu.
Ƙayyadaddun Sabis
Yawan jama'a:
Rarraba zuri'ar iyaye tare da mabambantan dabi'u.
misali zuriyar F2, Backcrossing (BC), Recombinant inbred line(RIL)
Wajen hadawa
Don halaye masu inganci: 30 zuwa 50 mutane (mafi ƙarancin 20) / girma
Don ƙididdigar ƙididdiga: saman 5% zuwa 10% mutane tare da ko dai matsananciyar yanayi a cikin dukan jama'a (mafi ƙarancin 30+30).
Shawarar zurfin jeri
Aƙalla 20X/iyaye da 1X/zuriya ɗaya (misali don zuriya ta hadawa ta 30+30, zurfin jeri zai zama 30X kowace girma)
Binciken bioinformatics
● Gabaɗayan kwayoyin halitta
● sarrafa bayanai
● Kiran SNP/Indel
● Binciken yanki na ɗan takara
● Bayanin aikin ɗan takara
Samfuran Bukatun da Bayarwa
Samfuran Bukatun:
Nucleotides:
| samfurin gDNA | Samfurin nama |
| Matsakaicin hankali: ≥30 ng/μl | Shuka: 1-2 g |
| Adadin: ≥2 μg (Juzu'in ≥15 μl) | Dabbobi: 0.5-1 g |
| Tsafta: OD260/280= 1.6-2.5 | Cikakken jini: 1.5 ml |
Gudun Aikin Sabis
Gwajin ƙira
Samfurin bayarwa
RNA cirewa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
1.Association bincike tushe a kan Euclidean Distance (ED) don gane dan takara yankin.A cikin adadi mai zuwa
X-axis: Lambar Chromosome;Kowace digo tana wakiltar ƙimar ED na SNP.Layin Baƙi yayi daidai da ƙimar ED mai dacewa.Ƙimar ED mafi girma tana nuna alaƙa mai mahimmanci tsakanin rukunin yanar gizon da phenotype.Layin jajayen dash yana wakiltar ƙofa na muhimmiyar ƙungiya.
2.Association analysis tushen babu SNP-index
X-axis: Lambar Chromosome;Kowace digo tana wakiltar ƙimar SNP-index.Layin baƙar fata yana tsaye don madaidaicin ƙimar SNP-index.Girman ƙimar shine, mafi girman mahimmancin ƙungiyar.
BMK Case
Babban tasiri mai ƙididdige ƙimar locus Fnl7.1 yana ɓoye ƙarancin furotin na embryogenesis mai alaƙa da tsayin wuyan 'ya'yan itace a cikin kokwamba.
Buga: Jaridar Biotechnology Journal, 2020
Dabarun tsarawa:
Iyaye (Jin5-508, YN): Dukkanin kwayoyin halitta masu kama da 34 × da 20 ×.
Wuraren DNA (50 Dogon wuyansa da 50 gajere-wuya): Sakamakon 61 × da 52 ×
Sakamako mai mahimmanci
A cikin wannan binciken, an samar da rarraba yawan jama'a (F2 da F2: 3) ta hanyar haye layin kokwamba mai tsayi Jin5-508 da YN gajere.An gina wuraren tafkunan DNA guda biyu ta wasu matsananci masu dogayen wuya 50 da matsananciyar gajerun wuyansa 50.An gano babban tasiri QTL akan Chr07 ta binciken BSA da taswirar QTL na gargajiya.An ƙara ƙaddamar da yankin ɗan takarar ta hanyar taswira mai kyau, ƙididdige maganganun kwayoyin halitta da gwaje-gwajen transgenic, wanda ya bayyana mahimmin kwayar halitta a cikin sarrafa tsayin wuyansa, CsFnl7.1.Bugu da ƙari, polymorphism a cikin CsFnl7.1 mai gabatarwa an gano yana da alaƙa da magana mai dacewa.Ƙarin bincike na phylogenetic ya ba da shawarar cewa wurin Fnl7.1 yana da yuwuwar ya samo asali daga Indiya.
 QTL-taswirar a cikin binciken BSA don gano yankin ɗan takarar da ke da alaƙa da tsayin wuyan kokwamba |  Bayanan bayanan LOD na QTL mai tsayin kokwamba da aka gano akan Chr07 |
Xu, X., et al."Babban tasiri mai ƙididdige ƙimar locus Fnl7.1 yana ɓoye ƙarancin furotin embryogenesis mai alaƙa da tsayin wuyan 'ya'yan itace a cikin kokwamba."Jaridar Biotechnology Journal 18.7(2020).
