Dogon Ƙididdigar Ƙimar-Illumina
Amfanin Sabis
●Binciken haɗin gwiwa na mRNA da lncRNA: ta hanyar haɗa ƙididdige ƙididdigar mRNA tare da nazarin lncRNA da maƙasudin su, yana yiwuwa a sami cikakken bayyani game da tsarin tsarin da ke ƙarƙashin amsawar salula.
●Ƙwararren Ƙwararru: Ƙungiyarmu tana kawo ƙwararrun ƙwarewa ga kowane aiki, tare da tarihin sarrafa samfuran sama da 23,000 a BMK wanda ke ɗaukar nau'ikan samfuri iri-iri da ayyukan lncRNA.
●Tsananin Kula da Inganci: Muna aiwatar da mahimman abubuwan sarrafawa a duk matakai, daga samfurin da shirye-shiryen ɗakin karatu zuwa jerin abubuwa da bioinformatics. Wannan saka idanu mai kyau yana tabbatar da isar da sakamako mai inganci akai-akai.
●Tallafin Bayan Talla: Alƙawarinmu ya wuce bayan kammala aikin tare da lokacin sabis na watanni 3 bayan-sayar. A wannan lokacin, muna ba da bibiyar aikin, taimako na warware matsala, da kuma zaman Q&A don magance duk wata tambaya da ta shafi sakamakon.
Samfuran Bukatun da Bayarwa
| Laburare | Dandalin | Bayanan da aka ba da shawarar | Bayanan Bayani na QC |
| rRNA ya ƙare ɗakin karatu na jagora | Farashin PE150 | 10-16 GB | Q30≥85% |
Nucleotides:
| Conc.(ng/μl) | Adadin (μg) | Tsafta | Mutunci |
| ≥ 80 | 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Iyakance ko babu furotin ko gurɓataccen DNA da aka nuna akan gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; iyakance ko babu girma daga tushe |
● Shuke-shuke:
Tushen, kara ko petal: 450 MG
Leaf ko iri: 300 MG
'Ya'yan itace: 1.2 g
● Dabbobi:
Zuciya ko hanji: 450 MG
Viscera ko Brain: 240 MG
tsoka: 600 MG
Kasusuwa, Gashi ko Fata: 1.5g
● Arthropods:
Kwari: 9g
Crustacea: 450 MG
● Cikakken jini:2 bututu
● Kwayoyin: 106 Kwayoyin
● Magani da Plasmaku: 6 ml
Isar da Samfurin Nasiha
Kwantena: 2 ml bututu centrifuge (Ba a ba da shawarar foil tin ba)
Alamar samfur: Ƙungiya+ kwafi misali A1, A2, A3; B1, B2, B3.
Kawo:
1. Busasshen ƙanƙara: Ana buƙatar samfurori a cikin jaka kuma a binne su a bushe-kankara.
2. RNAstable tubes: Ana iya bushe samfuran RNA a cikin bututun daidaitawar RNA (misali RNAstable®) kuma a tura su cikin zafin jiki.
Gudun Aikin Sabis
Gwajin ƙira
Samfurin bayarwa
RNA cirewa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
Bioinformatics
Bambance-bambancen Halin Halitta (DEGs).
Ƙididdigar lncRNA magana - tari
Ingantattun kwayoyin halittar lncRNA
MRNA haɗin gwiwa da nazarin matsayi na lncRNA - Makircin Circos (da'irar tsakiya shine mRNA kuma cicrlce na ciki shine lncRNA)
Bincika ci gaban da BMKGene's lncRNA equencing sabis ya sauƙaƙe ta hanyar tarin wallafe-wallafe.
Ji, H. et al. (2020) 'Ganewa, tsinkayar aiki, da maɓallin lncRNA tabbaci na lncRNA masu alaƙa da sanyi a cikin hanta beraye', Rahoton Kimiyya 2020 10: 1, 10 (1), shafi 1-14. doi: 10.1038/s41598-020-57451-7.
Jiya, Z. et al. (2021) 'Bincike na Haɗin Rubutun Yana Nuna Injin rigakafi don Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙarƙashin Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararru, 12, p. 687151. doi: 10.3389/FIMMU.2021.687151/BIBTEX.
Wang, XJ et al. (2022) 'Multi-Omics Integration-Bassed Pribased of Gasar Ƙarfin Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararru', Ƙwararrun Ƙwararru a Oncology, 12, p. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xiao, L. et al. (2020) 'Ratsawar kwayoyin halitta na cibiyar sadarwar haɗin gwiwar kwayoyin halitta da ke ƙarƙashin photosynthesis a cikin Populus', Jaridar Biotechnology Journal, 18(4), shafi. 1015-1026. doi: 10.1111/PBI.13270.
Zheng, H. et al. (2022) 'Cibiyar Sadarwar Yanar Gizo ta Duniya don Bayyanar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittar Halittu a cikin Kwayoyin rigakafi a cikin Microenvironment na Cutar Kabari da Hashimoto's Thyroiditis', Frontiers in Immunology, 13, p. 879824. doi: 10.3389/FIMMU.2022.879824/BIBTEX.
