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Takamaiman-Locus Amplified Sequencing Sequencing (SLAF-Seq)

Ƙwararren ƙwayar cuta mai girma, musamman a kan yawan jama'a, wani muhimmin mataki ne a cikin nazarin ƙungiyoyin kwayoyin halitta, wanda ke ba da tushen kwayoyin halitta don gano aikin kwayoyin halitta, nazarin juyin halitta, da dai sauransu. Maimakon zurfin sake sake tsarin kwayoyin halitta, rage yawan wakilcin kwayoyin halitta (RRGS). ) an gabatar da shi don rage farashin jeri akan kowane samfuri, yayin da ake kiyaye ingantaccen aiki akan gano alamun kwayoyin halitta.Ana samun wannan galibi ta hanyar ciro ɓangarorin taƙaitawa a cikin kewayon girman da aka ba, wanda ake kiran sunan ɗakin karatu na wakilci (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) dabara ce ta ci gaba da kai don SNP genotyping tare da ko ba tare da genome na tunani ba.
Platform: Illumina NovaSeq Platform


Cikakkun Sabis

Sakamakon Demo

Fitattun wallafe-wallafe

Cikakkun Sabis

Tsarin Fasaha

111

Gudun aiki

流程图

Amfanin Sabis

Babban ingancin gano alama- Fasahar jeri mai ƙima tana taimakawa SLAF-Seq wajen gano ɗaruruwan dubunnan tags a cikin dukkan kwayoyin halitta.

Low dogara ga kwayoyin halitta- Ana iya amfani da shi ga nau'in ko dai tare da ko ba tare da wani nau'i na genome ba.

Tsarin tsari mai sassauƙa- Single-enzyme, dual-enzyme, multi-enzyme narkewa da nau'ikan enzymes iri-iri, duk ana iya zaɓar su don biyan burin bincike daban-daban ko nau'in.Ana amfani da pre-kimanin a cikin siliki don tabbatar da ƙirar enzyme mafi kyau.

Ingantacciyar narkewar enzymatic- An gudanar da gwaje-gwaje na farko don inganta yanayin, wanda ke sa gwajin na yau da kullun ya tabbata kuma abin dogara.Ingancin tarin juzu'i na iya cimma sama da 95%.

Alamar SLAF da aka rarraba ko'ina- Ana rarraba alamun SLAF a ko'ina cikin duk chromosomes zuwa mafi girma, cimma matsakaicin 1 SLAF a kowace 4 kb.

Ingantacciyar gujewa maimaituwa- Matsakaicin maimaitawa a cikin bayanan SLAF-Seq ya ragu zuwa ƙasa da 5%, musamman a cikin nau'ikan da ke da babban matakin maimaitawa, kamar alkama, masara, da sauransu.

Kyawawan kwarewa-Sama da 2000 rufe ayyukan SLAF-Seq akan ɗaruruwan nau'ikan nau'ikan da ke rufe tsirrai, dabbobi masu shayarwa, tsuntsaye, kwari, ƙwayoyin ruwa, da sauransu.

Gudun aiki na bioinformatic da kansaBMKGENE ne ya haɓaka haɗe-haɗe na aikin bioinformatic don SLAF-Seq don tabbatar da aminci da daidaiton fitarwa na ƙarshe.

 

Ƙayyadaddun Sabis

 

Dandalin

Conc.(ng/gl)

Jimlar (ug)

OD260/280

Illumina NovaSeq

>35

>1.6(Juzu'i>15μl)

1.6-2.5

Lura: Samfura guda uku, kowanne tare da tsarin enzyme guda uku, za a yi don gwaji kafin gwaji.

Dabarun Jeri Na Shawarar

Zurfin jeri: 10X/Tag

Girman Halitta

Shawarar SLAF Tags

< 500 Mb

100K ko WGS

500 Mb- 1 GB

100 K

1 Gb-2 Gb

200 K

Giant ko hadadden kwayoyin halitta

300-400K

 

Aikace-aikace

 

Nasiha

Girman Yawan Jama'a

 

Dabarun tsarawa da zurfi

 

Zurfin

 

Lambar Tag

 

GWAS

 

Misalin lamba ≥ 200

 

10X

 

 

 

 

 

Bisa lafazin

girman kwayoyin halitta

 

Juyin Halitta

 

Mutanen kowannensu

Ƙungiya ≥ 10;

jimlar samfurori ≥ 30

 

10X

 

Isar da Samfurin Nasiha

Kwantena: 2 ml centrifuge tube

Don yawancin samfurori, muna ba da shawarar kada a adana a cikin ethanol.

Alamar samfurin: Samfuran suna buƙatar a yi musu lakabi a sarari kuma iri ɗaya ga siffan bayanin samfurin da aka ƙaddamar.

Jirgin ruwa: Busasshen ƙanƙara: Ana buƙatar a haɗa samfuran a cikin jakunkuna da farko kuma a binne su a bushe-kankara.

Gudun Aikin Sabis

Misalin QC
Gwajin matukin jirgi
Gwajin SLAF
Shirye-shiryen Laburare
Jeri
Binciken bayanai
Bayan Sabis na siyarwa

Misalin QC

Gwajin matukin jirgi

SLAF-gwaji

Shirye-shiryen Laburare

Jeri

Binciken Bayanai

Bayan-tallace-tallace Services


  • Na baya:
  • Na gaba:

  • 1. Kididdigar sakamakon taswira

    hoto1

    A1

    2. Ci gaban alamar SLAF

    A2

    3. Bambance-bambancen bayanin

    A3

    Shekara

    Jarida

    IF

    Take

    Aikace-aikace

    2022

    Sadarwar yanayi

    17.694

    Tushen genomic na giga-chromosomes da giga-genome na itacen peony

    Paeonia ostii

    SLF-GWAS

    2015

    Sabon Likitan Halitta

    7.433

    Sawun cikin gida yana kafa yankuna masu mahimmancin agronomic a cikin

    waken soya

    SLF-GWAS

    2022

    Jaridar Babban Bincike

    12.822

    G. hirsutum na Gossypium barbadense mai faɗin ɗan adam

    bayyana loci mafi girma don haɓaka ingancin fiber auduga da yawan amfanin ƙasa lokaci guda

    halaye

    SLAF-Genetics na Juyin Halitta

    2019

    Shuka Kwayoyin Halitta

    10.81

    Binciken Al'umma na Al'umma da Majalisar De Novo sun Bayyana Asalin Weedy

    Shinkafa a matsayin Wasan Juyin Halitta

    SLAF-Genetics na Juyin Halitta

    2019

    Halitta Halitta

    31.616

    Tsarin kwayoyin halitta da bambancin kwayoyin halittar irin kifi na kowa, Cyprinus carpio

    SLAF-Haɗin taswira

    2014

    Halitta Halitta

    25.455

    Halin halittar gyada da aka noma yana ba da haske game da legume karyotypes, polyploid

    juyin halitta da amfanin gona domestication.

    SLAF-Haɗin taswira

    2022

    Jaridar Biotechnology Journal

    9.803

    Gane ST1 yana bayyana zaɓin da ya haɗa da ƙwanƙwasa ƙwayoyin halittar iri

    da abun da ke cikin mai a lokacin gidan waken suya

    SLF-Marker ci gaba

    2022

    Jaridar Duniya ta Kimiyyar Kwayoyin Halitta

    6.208

    Ganewa da Ci gaban Alamar DNA don Alkama-Leymus mollis 2Ns (2D)

    Sauya Disomic Chromosome

    SLF-Marker ci gaba

    samun zance

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