Single-nucleus RNA Sequencing

Siffar RNA guda ɗaya da aka Fitar da Hoton

Ci gaba a cikin ɗaukan tantanin halitta guda ɗaya da dabarar ginin ɗakin karatu na ɗaiɗaikun haɗe tare da babban tsarin aiki yana ba da damar nazarin bayanin kwayoyin halitta akan tushen tantanin halitta.Yana ba da damar zurfafa da cikakken bincike na tsarin akan hadaddun yawan adadin tantanin halitta, wanda a cikinsa ya fi nisantar rufe nau'ikan halittarsu ta hanyar ɗaukar matsakaicin dukkan ƙwayoyin.

Duk da haka, wasu sel ba su dace a sanya su su zama dakatarwar tantanin halitta guda ɗaya ba, don haka wasu hanyoyin shirye-shiryen samfurin - ana buƙatar cirewar tsakiya daga kyallen takarda, wato, tsakiya ana fitar da su kai tsaye daga kyallen takarda ko tantanin halitta kuma an shirya su zuwa dakatarwar tsakiya guda ɗaya don guda ɗaya- jerin kwayoyin halitta.

BMK yana ba da sabis na jeri na 10x Genomics ChromiumTM tushen RNA guda tantanin halitta.An yi amfani da wannan sabis ɗin sosai a cikin binciken akan binciken da ke da alaƙa da cututtuka, irin su bambance-bambancen ƙwayoyin cuta, nau'in ƙwayar cuta, haɓaka nama, da sauransu.

Na'urar kwafin sararin samaniya: 10 × Genomics

Platform: Illumina NovaSeq Platform

Tuntube Mu

Cikakkun Sabis

Bioinformatics

Sakamakon Demo

Ƙa'idar fasaha

Ana samun keɓantawar ƙwayoyin cuta ta 10 × Genomics Chromium TM, wanda ya ƙunshi tsarin microfluidics na tashoshi takwas tare da ketare biyu.A cikin wannan tsarin, gel beads tare da barcodes da primer, enzymes da kuma tsakiya guda daya suna kunshe a cikin nanoliter-sized man drop, samar da Gel Bead-in-Emulsion (GEM).Da zarar an kafa GEM, ana yin sel lysis da sakin lambobin barcode a kowane GEM.mRNA ana jujjuya su cikin kwayoyin cDNA tare da 10x barcodes da UMI, waɗanda ke da alaƙa da daidaitaccen ginin ɗakin karatu.

Nama bai dace da shirye-shiryen dakatarwar tantanin halitta guda ɗaya ba

Cell / Nama	Dalili
Cire daskararre nama	Ba za a iya samun sabo ko ƙungiyoyi masu dogon lokaci ba
Kwayoyin tsoka, Megakaryocyte, Fat…	Diamita na salula ya yi girma da yawa don shigar da kayan aiki
Hanta…	Yayi rauni sosai don karyewa, ya kasa bambance sel guda ɗaya
Kwayoyin Neuron, Brain…	Ƙarin hankali, mai sauƙin damuwa, zai canza sakamakon jerin abubuwan
Pancreas, thyroid…	Mawadaci a cikin enzymes na endogenous, yana shafar samar da dakatarwar tantanin halitta

Single-nucleus vs Single-cell

Single-nucleus	Single-cell
Diamita na tantanin halitta mara iyaka	Diamita na salula: 10-40 μm
Kayan na iya zama daskararre nama	Dole ne kayan ya zama sabo ne
Low danniya na daskararre Kwayoyin	Maganin Enzyme na iya haifar da halayen damuwa na cell
Babu jajayen ƙwayoyin jini da ake buƙatar cirewa	Ana buƙatar cire jajayen ƙwayoyin jini
Nukiliya yana bayyana bayanan halittu	Dukan tantanin halitta yana bayyana bayanan halitta