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mRNA-seq (NGS) tare da kwayar halittar da aka ambata

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mRNA-seq (NGS) tare da kwayar halittar da aka ambata

RNA-seq kayan aiki ne na yau da kullun a fannin kimiyyar rayuwa da amfanin gona, wanda ke cike gibin da ke tsakanin kwayoyin halitta da kuma sunadarai. Ƙarfinsa ya ta'allaka ne a gano sabbin bayanai da kuma auna yadda suke bayyana a cikin gwaji ɗaya. Ana amfani da shi sosai don nazarin kwafi na kwatantawa, yana haskaka kwayoyin halitta da suka shafi halaye ko siffofi daban-daban, kamar kwatanta maye gurbi da nau'ikan daji ko bayyana bayyanar kwayoyin halitta a ƙarƙashin takamaiman yanayi. BMKCloud mRNA (Reference) APP yana haɗa ƙididdigar magana, nazarin bambancin magana (DEG), da kuma nazarin tsarin jeri a cikin bututun bioinformatics na mRNA-seq (NGS) kuma yana haɗa ƙarfin irin wannan software, yana tabbatar da dacewa da sauƙin amfani. Masu amfani za su iya loda bayanan RNA-seq ɗinsu zuwa gajimare, inda App ɗin ke ba da cikakken bayani game da bioinformatic mai cikakken tsayawa ɗaya. Bugu da ƙari, yana fifita ƙwarewar abokin ciniki, yana ba da ayyukan da aka keɓance waɗanda aka tsara don takamaiman buƙatun masu amfani. Masu amfani za su iya saita sigogi da ƙaddamar da aikin bututun da kansu, duba rahoton hulɗa, duba bayanai/zane-zane da cikakken haƙar bayanai, kamar: zaɓin kwayar halitta mai manufa, rarraba aiki, zane-zane, da sauransu.

Sakamakon gwaji
Haƙar bayanai
Bukatar shigo da kaya
Babban bincike
Nassoshi
Sakamakon gwaji

Haƙar bayanai

Bukatar shigo da kaya

Dandalin:Illumina, MGI
Dabaru:RNA-Seq
Tsarin Zane: An yi masa bayani mai tsabta, mai tsabta.
Nau'in ɗakin karatu:fr-unstrand, fr-firststrand ko fr-secondstrand
Tsawon karatu:150 bp
Nau'in fayil:*.fastq, *.fq, *.fastq.gz ko *.fq.gz. Tsarin zaita atomatik haɗa fayilolin .fastq bisa ga sunayen fayilolinsu,misali *_1.fastq an haɗa shi da *._2.fastq.
Adadin samfuran:Babu wani ƙayyadadden adadin da aka ƙayyadena samfurori, amma lokacin bincike zai ƙaru yayin da adadinsamfurori suna girma.
Adadin Bayanan da aka Ba da Shawara:6G a kowace samfuri

Babban bincike
Babban kayan aikin bincike da bioinformatic na mRNA-seq (Reference)bututun bututun kamar haka:
1. Kula da Ingancin Rawdata:
• Cire jerin masu ƙarancin inganci, jerin adaftar,da sauransu;
•Kayan aiki: bututun da aka gina a cikin gida;
2. Daidaita bayanai zuwa ga kwayar halittar da aka yi amfani da ita:
• Daidaita karatu tare da tsarin haɗin gwiwa akan tsarinkwayar halittar da aka yi amfani da ita.
•Kayan aiki:HISAT2, samtools
3. Binciken ingancin ɗakin karatu:
• Saka nazarin tsayi, nazarin jikewa a jere, da sauransu;
•Kayan aiki:samtools;
4. Binciken tsarin jeri:
•Madadin nazarin haɗakar kwayoyin halitta, inganta tsarin kwayoyin halitta,sabon hasashen kwayoyin halitta, da sauransu;
•Kayan aiki:Tire-tire, kwatanta, GATK,LU'AMOND, InterProScan, kumaHMMER.
5. Binciken bambancin magana:
• Tantance DEG, nazarin alaƙar haɗin gwiwa, aikiwadata;
Sakamakon gani daban-daban;
Rtare daSEGseq, DESeq2, ggplot2, DEXSeq
Nassoshi
1. Kim, Daehwan et al. “Daidaitaccen tsarin kwayoyin halitta bisa zane da kumayin amfani da tsarin halittar HISAT2 da HISAT-genotype.YanayiFasahar kere-kere37 (2019): 907 - 915.
2. McKenna, Aaron da sauransu. “Kayan Aikin Nazarin Halittar Jini: aTsarin MapReduce don nazarin DNA na ƙarni na gabajerin bayanai.Binciken kwayoyin halitta20 9 (2010): 1297-303.
3. Li, Heng da sauransu. “Tsarin Jerin/Taswirar daSAMtools.Bioinformatics25 (2009): 2078 - 2079.
4. Perțea, Mihaela et al. “StringTie yana ba da damar ingantawasake gina wani rubutu daga karatun jerin RNA.YanayiFasahar kere-kere33 (2015): 290-295.
5. Love, Michael I. da sauransu. “Kimantawar da aka daidaita na canjin lanƙwasa da kumawatsawa don bayanan RNA-seq tare da DESeq2.Kwayoyin halittaIlimin halittu15 (2014): n. shafi.
6. Eddy, Sean R.. “Binciken HMM na Bayanan Sirri Mai Sauri.”PLoS Ilimin Halittar Kwamfuta7 (2011): n. shafi.

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