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Binciken Ƙungiya mai Faɗi
Nazarin ƙungiyar genome-wide (GWAS) yana nufin gano bambance-bambancen kwayoyin halitta (genotype) waɗanda ke da alaƙa da takamaiman halaye (phenotype).Nazarin GWAS yana bincika alamomin kwayoyin halitta sun haye dukkanin kwayoyin halittar mutane masu yawa kuma yana tsinkayar ƙungiyoyin genotype-phenotype ta ƙididdigar ƙididdiga a matakin yawan jama'a.An yi amfani da shi sosai a cikin bincike kan cututtukan ɗan adam da aikin haƙar ma'adinan kwayoyin halitta akan hadaddun halaye na dabbobi ko tsirrai.
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Single-nucleus RNA Sequencing
Ci gaba a cikin ɗaukan tantanin halitta guda ɗaya da dabarar ginin ɗakin karatu na ɗaiɗaikun haɗe tare da babban tsarin aiki yana ba da damar nazarin bayanin kwayoyin halitta akan tushen tantanin halitta.Yana ba da damar zurfafa da cikakken bincike na tsarin akan hadaddun yawan adadin tantanin halitta, wanda a cikinsa ya fi nisantar rufe nau'ikan halittarsu ta hanyar ɗaukar matsakaicin dukkan ƙwayoyin.
Duk da haka, wasu sel ba su dace a sanya su su zama dakatarwar tantanin halitta guda ɗaya ba, don haka wasu hanyoyin shirye-shiryen samfurin - ana buƙatar cirewar tsakiya daga kyallen takarda, wato, tsakiya ana fitar da su kai tsaye daga kyallen takarda ko tantanin halitta kuma an shirya su zuwa dakatarwar tsakiya guda ɗaya don guda ɗaya- jerin kwayoyin halitta.
BMK yana ba da sabis na jeri na 10x Genomics ChromiumTM tushen RNA guda tantanin halitta.An yi amfani da wannan sabis ɗin sosai a cikin binciken akan binciken da ke da alaƙa da cututtuka, irin su bambance-bambancen ƙwayoyin cuta, nau'in ƙwayar cuta, haɓaka nama, da sauransu.
Na'urar kwafin sararin samaniya: 10 × Genomics
Platform: Illumina NovaSeq Platform
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Tsarin Tsiro/Dabbobi Gabaɗayan Halitta
Dukkanin tsarin sake-sake-tsarin kwayoyin halitta, wanda kuma aka sani da WGS, yana ba da damar bayyanar da sauye-sauye na yau da kullun da na yau da kullun akan dukkan kwayoyin halittar da suka hada da Single Nucleotide Polymorphism (SNP), Sharewa Shiga (InDel), Bambancin Tsarin (SV), da Bambancin Lamba Kwafi (CNV). ).SVs sun ƙunshi babban ɓangare na tushen bambancin fiye da SNPs kuma suna da tasiri mafi girma akan kwayoyin halitta, wanda ke da tasiri mai mahimmanci akan rayayyun halittu.Tsawon karatu mai tsawo yana ba da damar ƙarin ainihin gano manyan gutsuttsura da bambance-bambance masu rikitarwa saboda dogon karantawa yana ba da sauƙin hayewar chromosomal akan yankuna masu rikitarwa kamar maimaita tandem, yankuna masu wadatar GC/AT, da yankuna masu saurin canzawa.
Platform: Illumina, PacBio, Nanopore
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BMKMANU S1000 Spatial Transcriptome
Tsarin sararin samaniya na sel yana taka muhimmiyar rawa a cikin matakai daban-daban na ilimin halitta, kamar shigar da rigakafi, haɓaka amfrayo, da dai sauransu. Tsarin fassarar sararin samaniya, wanda ke nuna bayanan bayanan kwayoyin halitta yayin da yake riƙe da bayanan sararin samaniya, ya ba da haske mai zurfi game da gine-ginen matakin nama.Tare da haɓakar fasaha, ƙwararrun ƙwayoyin nama mai haske da ainihin bambancin tsarin yanayin magana yana buƙatar yin nazari tare da ƙuduri mafi girma.BMKGENE yana ba da cikakkiyar sabis ɗin jujjuya bayanan sarari na tsayawa ɗaya daga samfurori zuwa fahimtar ilimin halitta.
Fassarar fasikanci na sararin samaniya sun ba da ƙarfin hangen nesa na labari a fagen bincike daban-daban ta hanyar warware bayanin bayanan kwayoyin halitta tare da abun ciki na sararin samaniya a cikin samfurori iri-iri.
guntu kwafin sararin samaniya: BMKMANU S1000
Platform: Illumina NovaSeq Platform
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10x Genomics Visium Spatial Transcriptome
Maganar Visium Spatial Gene Expression fasaha ce ta gama gari ta juzu'i don rarraba nama dangane da jimillar mRNA.Yi taswirar gabaɗayan rubutun tare da mahallin ilimin halittar jiki don gano sabbin dabaru game da haɓakawa na yau da kullun, cututtukan cututtuka, da binciken fassarar asibiti.BMKGENE yana ba da cikakkiyar sabis ɗin jujjuya bayanan sarari na tsayawa ɗaya daga samfurori zuwa fahimtar ilimin halitta.
Fasahar fasikanci ta sararin samaniya sun ba da ƙarfin hangen nesa na labari a fagen bincike daban-daban ta hanyar warware bayanan bayanan kwayoyin halitta tare da abun ciki na sararin samaniya a cikin samfurori iri-iri..
Na'urar kwafin sararin samaniya: 10x Genomics Visium
Dandalin:Illumina NovaSeq Platform
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Cikakkun Tsawon mRNA Sequencing-Nanopore
Jerin RNA ya kasance kayan aiki mai kima don cikakken bincike na kwafi.Babu shakka, jerin gajerun karatun gargajiya sun sami ci gaba mai yawa a nan.Duk da haka, sau da yawa yakan gamu da iyakancewa a cikin cikakkun bayanan isoform na tsawon tsayi, ƙididdigewa, son zuciya na PCR.
Nanopore sequencing ya bambanta kansa da sauran tsarin dandali, domin ana karanta nucleotide kai tsaye ba tare da haɗin DNA ba kuma yana haifar da dogon karatu a dubun kilobases.Wannan yana ba da ikon ƙetare cikakken kwafin kwafin karatu kai tsaye da kuma magance ƙalubalen a cikin karatun matakin isoform.
Dandalin:Nanopore PromethION
Laburare:cDNA-PCR
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Tsarin mRNA mai cikakken tsayi -PacBio
Da novojeri mai cikakken tsayin rubutu, wanda kuma aka sani daDa novoIso-Seq yana ɗaukar fa'idodin PacBio sequencer a cikin tsayin karantawa, wanda ke ba da damar jeri na cikakken tsawon kwayoyin cDNA ba tare da wani hutu ba.Wannan gaba ɗaya yana guje wa duk wani kurakurai da aka haifar a cikin matakan tattara bayanan rubutu kuma yana gina saiti na unigene tare da ƙudurin matakin isoform.Wannan saitin unigene yana ba da bayanan kwayoyin halitta masu ƙarfi a matsayin "kwayoyin halitta" a matakin rubutu.Bugu da kari, haɗe tare da bayanan jerin tsararru na gaba, wannan sabis ɗin yana ba da ƙarfin ƙididdige ƙimar matakin isoform.
Platform: PacBio Sequel IILibrary: SMRT bell library -
Eukaryotic mRNA Sequencing-Illumina
Tsarin mRNA yana ba da damar yin bayanin duk mRNAs da aka rubuta daga sel a ƙarƙashin takamaiman yanayi.Fasaha ce mai ƙarfi don bayyana bayanan bayanan kwayoyin halitta, tsarin kwayoyin halitta da hanyoyin kwayoyin wasu hanyoyin nazarin halittu.Ya zuwa yau, an yi amfani da jeri na mRNA a cikin bincike na asali, bincike na asibiti, haɓaka magunguna, da sauransu.
Platform: Illumina NovaSeq Platform
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Matsakaicin tushen mRNA Sequencing-Illumina
mRNA sequencing ya rungumi dabara na gaba-gaba (NGS) don kama manzo RNA (mRNA) ya samar da Eukaryote a takamaiman lokacin da wasu ayyuka na musamman ke kunnawa.Mafi tsayin rubutun da aka raba ana kiransa 'Unigene' kuma an yi amfani da shi azaman jeri na bincike na gaba, wanda shine ingantacciyar hanya don nazarin tsarin kwayoyin halitta da tsarin sadarwa na nau'in ba tare da tunani ba.
Bayan tattara bayanan kwafi da bayanin aikin unigene
(1) Binciken SSR, tsinkayar CDS da tsarin kwayoyin halitta za a riga an tsara su.
(2) Ƙididdigar maganganun unigene a cikin kowane samfurin za a yi.
(3)Unigenes daban-daban da aka bayyana tsakanin samfurori (ko ƙungiyoyi) za a gano su bisa lafazin unigene
(4) Za a gudanar da tari, bayanin aiki da kuma ingantaccen bincike na unigenes da aka bayyana daban-daban.
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Dogon jerin abubuwan da ba codeing-Ilumina
Dogayen RNAs marasa coding (lncRNAs) wani nau'in kwayoyin RNA ne masu tsayin da ya wuce 200 nt, wadanda aka siffata su da yuwuwar yin coding.LncRNA, a matsayin maɓalli mai mahimmanci a cikin RNAs mara coding, ana samunsa galibi a cikin tsakiya da plasma.Haɓakawa a cikin jerin abubuwan fasaha da bioinformtics suna ba da damar gano lncRNAs da yawa da kuma haɗa waɗanda ke da ayyukan ilimin halitta.Shaidu masu tarawa suna ba da shawarar cewa lncRNA yana da hannu sosai a cikin ƙa'idodin epigenetic, ka'idojin rubutun da kuma tsarin rubutun bayan bayanan.
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Ƙananan jerin RNA-Illumina
Ƙananan RNA yana nufin nau'in kwayoyin RNA marasa coding waɗanda yawanci ba su wuce 200nt tsayi ba, gami da micro RNA (miRNA), ƙaramin tsangwama RNA (siRNA), da RNA (piRNA) mai hulɗa da piwi.
MicroRNA (miRNA) aji ne na ƙananan RNA mai ƙarfi tare da tsawon kusan 20-24nt, wanda ke taka muhimmiyar rawa na tsari iri-iri a cikin sel.miRNA ya shiga cikin matakai na rayuwa da yawa waɗanda ke bayyana nama - takamaiman da mataki - takamaiman magana kuma an kiyaye su sosai a cikin nau'ikan daban-daban.
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circRNA sequencing-Ilumina
CircRNAs (RNAs madauwari) aji ɗaya ne na ƙwayoyin RNA marasa coding waɗanda ke da gajeriyar hular ƙarshen 5' da 3' ƙarshen poly(A).CircRNAs suna yin tsarin madauwari ta hanyar haɗin gwiwa wanda shine matsayi akan narkewar RNA exonuclease.circRNA tana taka muhimmiyar rawa wajen haɓakawa da haɓakar halittu da juriya ga yanayin waje.
CircRNA yana da ayyuka da yawa, waɗanda zasu iya haɗawa da miRNA cikin gasa, aiwatar da aikin ka'ida na ceRNA, don daidaita maganganun kwayoyin halitta.A cikin 'yan shekarun nan, an kuma gano cewa yana da alaƙa da abubuwan da suka faru da ci gaban cututtuka, kuma yana da kyakkyawan fata na aikace-aikace a cikin hanyar gano alamun cututtuka da sauransu.
