Prokaryotic RNA Sequencing
Siffofin
● Sarrafa samfurin RNA ya haɗa da raguwar rRNA tare da shirye-shiryen ɗakin karatu na RNA.
● Binciken bioinformatic dangane da daidaitawa zuwa kwayoyin halitta
● Bincike ya haɗa da maganganun kwayoyin halitta da DEGs amma kuma tsarin kwafi da kuma nazarin sRNA
Amfanin Sabis
●Tsananin Kula da Inganci: muna aiwatar da mahimman abubuwan sarrafawa a duk matakai, daga samfurin da shirye-shiryen ɗakin karatu zuwa jerin abubuwa da bioinformatics. Wannan saka idanu mai kyau yana tabbatar da isar da sakamako mai inganci akai-akai.
●Ƙayyadaddun bayanan jeri na Strand: saboda shirye-shiryen ɗakin karatu na RNA kasancewa jagora, yana ba da damar gano bayanan da ba su da hankali.
●Cikakken Nazari Wanda Aka Keɓance Zuwa Rubutun Prokaryotic: bututun bioinformatic ya haɗa da ba kawai nazarin maganganun kwayoyin halitta ba har ma da nazarin tsarin rubutun, ciki har da ganewar operons, UTRs da masu gabatarwa. Hakanan ya haɗa da nazarin sRNAs, wato annotation da tsinkayar tsarin sakandare da maƙasudai.
●Tallafin Bayan Talla: alkawarinmu ya wuce bayan kammala aikin tare da lokacin sabis na watanni 3 bayan-sayar. A wannan lokacin, muna ba da bibiyar aikin, taimako na warware matsala, da kuma zaman Q&A don magance duk wata tambaya da ta shafi sakamakon.
Samfuran Bukatun da Bayarwa
| Laburare | Dabarun jeri | An ba da shawarar bayanai | Kula da inganci |
| rRNA ya ƙare ɗakin karatu na jagora | Farashin PE150 | 1-2 Gb | Q30≥85% |
Samfuran Bukatun:
| Conc.(ng/μl) | Adadin (μg) | Tsafta | Mutunci |
| ≥ 50 | ≥ 1 | OD260/280=1.8-2.0 OD260/230=1.0-2.5 Iyakance ko babu furotin ko gurɓataccen DNA da aka nuna akan gel. | RIN≥6.5 |
Isar da Samfurin Nasiha
Kwantena: 2 ml bututu centrifuge (Ba a ba da shawarar foil tin ba)
Alamar samfur: Ƙungiya+ kwafi misali A1, A2, A3; B1, B2, B3.
Kawo:
1. Busasshen ƙanƙara: Ana buƙatar samfurori a cikin jaka kuma a binne su a bushe-kankara.
2. RNAstable tubes: Ana iya bushe samfuran RNA a cikin bututun daidaitawar RNA (misali RNAstable®) kuma a tura su cikin zafin jiki.
Gudun Aikin Sabis
Samfurin bayarwa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
Gudun Ayyuka na Bioinformatic Analysis
Ya haɗa da Binciken Mai zuwa:
● Raw data ingancin kula
● Daidaita zuwa ga kwayoyin halitta
● Ƙimar ingancin Laburare: Bazuwar ɓarkewar RNA, saka girman da jikewa jeri.
● Bayanin aiki na ƙwayoyin cuta da aka annabta
● Binciken Magana: Daidaituwa da Babban Binciken Abubuwan Mahimmanci (PCA)
● Bambance-bambancen Halitta (DEGs)
● Bayanin aiki da haɓaka DEGs
● Binciken sRNA: tsinkaya, bayani, manufa, da tsinkayar tsari na biyu
● Ƙididdigar Tsarin Rubuce-rubuce: operons, farawa da matsayi na ƙarshe, Yankin da ba a fassara ba (UTS), mai gabatarwa, da kuma nazarin SNP/InDel
Sequencing jikewa
Bayanin aiki na codeing genes
Daidaita tsakanin samfurori
Binciken Bambancin Bayyana Halitta (DEGs).
Binciken haɓaka aikin aiki
sRNA annotation
Bincika ci gaban da BMKGene's Nanopore cikakken tsawon sabis na jerin mRNA ya samu a cikin wannan fitaccen ɗaba'ar.
Guan, CP et al. (2018)' Canje-canjen Rubutun Duniya na Biofilm-Kafa Staphylococcus epidermidis Mai amsawa ga Jimillar Alkaloids na Sophorea alopecuroides',Jaridar Yaren mutanen Poland na Microbiology, 67(2), shafi. 223. doi: 10.21307/PJM-2018-024.
