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Microbiomics

  • Tsarin Metagenomic -NGS

    Tsarin Metagenomic -NGS

    Metagenome yana nufin tarin tarin kwayoyin halitta na gauraya al'umma, kamar muhalli metagenome, mutum metagenome, da dai sauransu. Yana dauke da kwayoyin halittun da ake nomawa da wadanda ba za a iya nomawa ba.Tsarin metagenomic kayan aiki ne na kwayoyin halitta da ake amfani da shi don nazarin gaurayen kayan halitta da aka samo daga samfuran muhalli, wanda ke ba da cikakkun bayanai game da bambancin nau'in da yawa, tsarin yawan jama'a, dangantakar phylogenetic, kwayoyin aiki da hanyar sadarwa tare da abubuwan muhalli.

    Dandalin:Illumina NovaSeq Platform

  • Tsarin Metagenomic-Nanopore

    Tsarin Metagenomic-Nanopore

    Metagenomics wani kayan aiki ne na kwayoyin halitta da ake amfani dashi don nazarin abubuwan da aka haɗe da kwayoyin halitta da aka samo daga samfurori na muhalli, wanda ke ba da cikakkun bayanai game da bambancin nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i) nau'i-nau'i-nau'i na kwayoyin da ake amfani da su don nazarin abubuwan da suka shafi muhalli,da sauransu. zuwa nazarin metagenomic.Fitaccen aikin sa a cikin tsayin karantawa ya inganta binciken metagenomic na rafi, musamman taron metagenome.Yin amfani da fa'idodin tsayin karantawa, nazarin metagenomic na tushen Nanopore yana iya samun ƙarin ci gaba da taro idan aka kwatanta da abubuwan metagenomics na harbi.An buga cewa Nanopore na tushen metagenomics sun sami nasarar haifar da cikakkun kuma rufaffiyar kwayoyin halittar kwayoyin cuta daga microbiomes (Moss, EL, et. al,Nature Biotech, 2020)

    Dandalin:Nanopore PromethION P48

  • 16S/18S/ITS Amplicon Sequencing-PacBio

    16S/18S/ITS Amplicon Sequencing-PacBio

    Rukunin da ke kan 16S da 18S rRNA wanda ya ƙunshi yankuna biyun da aka kiyaye su sosai da kuma masu saurin canzawa shine cikakken hoton yatsa na kwayoyin halitta don gano kwayoyin prokaryotic da eukaryotic.Shan amfani da sequencing, wadannan amplicons za a iya niyya dangane da kiyaye sassa da kuma hyper-canzawa yankuna za a iya cikakken hali ga microbial ganewa na taimaka wa nazarin rufe microbial bambancin bincike, taxonomy, phylogeny, da dai sauransu Single-kwayoyin real-lokaci (SMRT). Tsarin tsarin dandamali na PacBio yana ba da damar samun ingantaccen dogon karantawa, wanda zai iya rufe amplicons masu tsayi (kimanin 1.5 Kb).Faɗin ra'ayi game da filin kwayoyin halitta ya inganta ƙudiri sosai a cikin bayanin nau'in ƙwayoyin cuta ko fungi.

    Dandalin:PacBio Sequel II

  • 16S/18S/ITS Amplicon Sequencing-NGS

    16S/18S/ITS Amplicon Sequencing-NGS

    16S/18S/ITS amplicon sequencing da nufin bayyana phylogeny, haraji, da nau'i mai yawa a cikin microbial al'umma ta hanyar binciken PCR kayayyakin na kiyaye gida alamomin kwayoyin halitta wanda ya ƙunshi duka biyu hira sosai da kuma hypervariable sassa.Gabatar da waɗannan cikakkiyar sawun yatsa na ƙwayoyin cuta ta Woeses et al, (1977) yana ba da damar keɓance bayanan microbiome mara amfani.Jeri na 16S (kwayoyin cuta), 18S (fungi) da na waje da aka rubuta na ciki (ITS, fungi) yana ba da damar gano nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan iri biyu ne, da nau'ikan da ba a san su ba.Wannan fasaha ta zama kayan aiki da yawa da ake amfani da su kuma babban kayan aiki don gano nau'ikan ƙwayoyin cuta daban-daban a cikin yanayi daban-daban, kamar bakin ɗan adam, hanji, najasa, da sauransu.

    Dandalin:Illumina NovaSeq Platform

  • Bacterial da Fungal Dukan Halittun Halittu Sake-Sequencing

    Bacterial da Fungal Dukan Halittun Halittu Sake-Sequencing

    Bacterial da fungal dukan kwayoyin halittar sake-sequencing kayan aiki ne mai mahimmanci don kammala kwayoyin halittar ƙwayoyin cuta da fungi da aka sani, da kuma kwatanta kwayoyin halitta masu yawa ko yin taswirar kwayoyin halitta na sababbin kwayoyin halitta.Yana da matukar muhimmanci a jera dukkan kwayoyin halittar kwayoyin cuta da fungi domin samar da ingantattun kwayoyin halittar kwayoyin halitta, don yin gano kwayoyin halitta da sauran nazarin kwayoyin halittar kwatance.

    Platform: Illumina NovaSeq Platform

  • Metatranscriptome Sequencing

    Metatranscriptome Sequencing

    Metatranscriptome sequencing yana nuna bayanin kwayoyin halitta na microbes (duka eukaryotes da prokaryotes) a cikin mahalli na halitta (watau ƙasa, ruwa, teku, najasa, da gut). Naƙiƙa, nazarin ƙirƙirar halittu daban-daban da aka bayyana halittu, da ƙari.

    Platform: Illumina NovaSeq Platform

  • Fungal Genome

    Fungal Genome

    Biomarker Technologies suna samar da binciken kwayoyin halitta, kyawawan kwayoyin halitta da kuma cikakkiyar kwayar halittar pene na fungal dangane da takamaiman manufar bincike.Za'a iya samun jerin abubuwan da ke tattare da kwayoyin halitta, taro da bayanin aiki ta hanyar haɗa jerin tsaran gaba + Tsarin tsara na uku don cimma babban taro na genome.Hakanan ana iya amfani da fasahar Hi-C don sauƙaƙe haɗuwar kwayoyin halitta a matakin chromosome.

    Dandalin:PacBio Sequel II

    Nanopore PromethION P48

    Illumina NovaSeq Platform

  • Bacteria Complete Genome

    Bacteria Complete Genome

    Biomarker Technologies yana ba da sabis na jeri akan gina cikakkiyar kwayar halittar ƙwayoyin cuta tare da gibin sifili.Babban aikin ƙwayoyin cuta cikakken ginin kwayoyin halitta ya haɗa da jerin tsararrun tsararraki na uku, taro, bayanin aiki da ci-gaba na nazarin halittu masu cika takamaiman manufofin bincike.Ƙarin cikakkun bayanan kwayoyin halitta na kwayoyin halitta yana ba da damar bayyana mahimman hanyoyin da ke ƙarƙashin tsarin rayuwarsu, wanda kuma zai iya ba da mahimmanci ga bincike na kwayoyin halitta a cikin nau'in eukaryotic mafi girma.

    Dandalin:Nanopore PromethION P48 + Illumina NovaSeq Platform

    PacBio Sequel II

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