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Kayayyaki

Chromatin Immunoprecipitation Sequencing (ChiP-seq)

Hotunan da aka Fitar da Chromatin (ChIP-seq).
  • Chromatin Immunoprecipitation Sequencing (ChiP-seq)
  • Chromatin Immunoprecipitation Sequencing (ChiP-seq)

ChIP-Seq yana ba da faffadan bayanan kwayoyin halittar DNA don gyare-gyaren tarihi, abubuwan rubutawa, da sauran sunadaran da ke da alaƙa da DNA.Yana haɗuwa da zaɓi na chromatin immuno-hazo (ChiP) don dawo da takamaiman rukunin furotin-DNA, tare da ikon jerin tsararru na gaba (NGS) don babban tsarin aiwatarwa na DNA da aka gano.Bugu da ƙari, saboda an dawo da rukunin furotin-DNA daga sel masu rai, ana iya kwatanta wuraren ɗaure a nau'ikan tantanin halitta da kyallen takarda, ko ƙarƙashin yanayi daban-daban.Aikace-aikace sun bambanta daga ƙa'idodin rubuce-rubuce zuwa hanyoyin haɓakawa zuwa hanyoyin cututtuka da ƙari.

Platform: Illumina NovaSeq Platform


Cikakkun Sabis

Bioinformatics

Sakamakon Demo

Amfanin Sabis

● Platform: Illumina NovaSeq Platform, PE150

● Nau'in ɗakin karatu: 100-500bp saka ɗakin karatu na DNA (ya dogara da mafi girman rarraba)

● Dabarar jeri: Haɗa-Ƙarshen 150 bp

● Ba da shawarar fitar da bayanai: 6 Gb raw bayanai/samfuri.

Samfuran Bukatun

Yawan DNA: ≥ 70 ng, babban kololuwar 100 zuwa 500 bp

Samfurin girma: ≥ 10 μl

OD260/280 = 1.8 zuwa 2.0 ba tare da lalacewa ko gurɓataccen RNA ba.

Gudun aikin gwaji

图片1

Gudun Aikin Sabis

samfurin bayarwa

Samfurin bayarwa

Shirye-shiryen Laburare

Gina ɗakin karatu

Jeri

Jeri

Binciken bayanai

Binciken bayanai

Bayan Sabis na siyarwa

Bayan-sayar da sabis

  • Na baya:
  • Na gaba:

    • 表观-01

    1. Kololuwar haɓaka haɓakawa

    官网图1

     

    2. MA makirci na bambancin kololuwa

    官网图2

     

    3.Rahoton da aka ƙayyade na KEGG

    官网图3

     

    4. GO rarrabawa

    官网图4

    samun zance

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    Danna shiga don bincika ko ESC don rufewa