条形 banner-03

Kayayyaki

Jerin DNA/RNA - Jerin Nanopore

Tsarin ONT wata fasaha ce ta siginar lantarki ta zamani wadda aka gina ta da nanopores, ƙa'idar tsarin kowane dandamali iri ɗaya ce. DNA/RNA mai layi biyu za ta ɗaure zuwa furotin nanoporos da aka saka a cikin biofilm ɗin kuma ta huta ƙarƙashin jagorancin furotin na mota, ƙarƙashin tasirin bambancin ƙarfin lantarki daga ɓangarorin biyu na biofilm ɗin, zaren DNA/RNA suna ratsa ta cikin furotin na tashar nanopore a wani ƙayyadadden adadin. Saboda bambance-bambancen halayen sinadarai na tushe daban-daban akan zaren DNA/RNA, lokacin da tushe ɗaya ko kwayar halittar DNA ta ratsa ta hanyar nanopore, zai haifar da canjin siginar lantarki daban-daban. Ta hanyar ganowa da daidaita waɗannan siginar, ana iya ƙididdige nau'ikan tushe masu dacewa, kuma ana iya kammala gano jerin a ainihin lokacin.


Cikakkun Bayanan Sabis

Sakamakon Gwaji

Siffofin Cikakkun Bayanan Sabis

Dandalin

Girman Laburare

Yawan Bayanan Ka'ida (Kowace Kwayar Halitta)

Daidaito na tushe ɗaya

Aikace-aikace

Nanopore

8Kb, 10kb, 20kb, Ultralong, cDNA-PCR

70-90Gb/Kwayar halitta

Kashi 85-92%

Kiran SV, De novo, Cikakken tsari, Iso-Seq, Bayanin Halitta, Gano methylation na DNA

Fa'idodin Sabis

● Fiye da shekaru 5 na gwaninta a kan dandamalin jerin PacBio tare da dubban ayyuka da aka rufe tare da nau'ikan halittu daban-daban.
● BMKGENE abokin tarayya ne na hukuma na Oxford Nanopore, tare da takardar shaidar RNA/DNA ta dandamali biyu.
● Akwai manyan samfuran na'urorin tsara jerin abubuwa tare da cikakken kayan aiki da isasshen tsarin tsara jerin abubuwa.
● Dangane da dandalin Nanopore, an buga fiye da bincike 10 na dabbobi da tsirrai na Denovo a cikin mujallu masu shahara a duniya.

Samfurin Bukatun


Nau'in Samfura

Adadin

Mayar da Hankali (Qubit ®)

Ƙarar girma

Tsarkaka

Wasu

DNA na kwayoyin halitta

Dogara da Bukatar Bayanai

 ≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5;

Haske mai haske a 260 nm, babu gurɓatawa

Ana buƙatar auna maida hankali ta hanyar Qubit da Qubit/Nanopore ≤ 2

Jimlar RNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5; babu gurɓatawa

Ƙimar RIN ≥7.5

 

Tsarin Aikin Sabis

shirye-shiryen samfurin

Shirye-shiryen samfurin

Shirye-shiryen Laburare

Gina ɗakin karatu

Jerin abubuwa

Jerin abubuwa

Nazarin bayanai

Nazarin bayanai

Samfurin QC

Isar da aikin


  • Na baya:
  • Na gaba:

  • Kimanta Ingancin Bayanai na Samfurin DNA

    Tebur 1. Ƙididdiga akan bayanai masu tsabta.

    BMKID

    rawSeqNum

    rawSumBase

    cleanSeqNum

    cleanSumBase

    cleanN50Len

    cleanN90Len

    cleanMeanLen

    cleanMaxLen

    cleanMeanQual

    DNA_BMK01

    1,218,239

    26.37

    1,121,736

    25.90

    28,014

    15,764

    23,090

    143,181

    9

    Kimanta Ingancin Bayanai na Samfurin RNA

    Tebur 1. Ƙididdiga akan bayanai masu tsabta.

    Sunan Fayil

    ID na Abokin Ciniki

    Lambobin Karatu

    BaseNum

    N50

    Matsakaicin Tsawon

    Matsakaicin Tsawon

    Ma'anar Ma'auni

    RNA_BMK001

    C2

    8,947,708

    4,047,230,083

    398

    452

    129,227

    Q12

    Siffa ta 1. Karanta rarrabawar tsawon

    A3

    Siffa ta 2. Rarraba ma'auni mai inganci na bayanai masu tsabta

    A4

    Siffa ta 3. Tsawo da rarraba maki mai kyau na bayanai masu tsabta

    A5

    sami ƙiyasin farashi

    Rubuta saƙonka a nan ka aika mana da shi

    Aika mana da sakonka: