Raupapa mRNA Eukaryotic-NGS
Ngā Āhuatanga
● Te whakarite whare pukapuka mRNA aronga whiriwhiri hei whakahohe i te whiwhi raraunga raupapatanga motuhake-miro
● Ka hipokina e ngā tātaritanga BI rerekē ngā hiahia katoa o ā mātou kiritaki
Ngā Painga
●Tohungatanga WhānuiNeke atu i te 600,000 ngā tauira kua tukatukahia e mātou, tae atu ki ngā momo tauira rerekē pērā i ngā ahurea pūtau, ngā kiko, me ngā wai tinana. Ka kawea mai e mātou te nui o ngā pūkenga ki ia kaupapa.
●Mana Kounga PūmauKa whakatinanahia e mātou ngā pūwāhi whakahaere matua puta noa i ngā wāhanga katoa, mai i te whakarite tauira ki te whakarite whare pukapuka, te raupapatanga me te hangarau koiora. Mā tā mātou aroturukitanga tino tupato ka puta he hua kounga teitei tonu.
●He Whakarāpopototanga WhānuiKa whakamahia e mātou ngā pātengi raraunga maha hei tāpiri i ngā mahi a ngā Ira Whakapuakitanga Rerekē (DEG) me te mahi i ngā tātaritanga whakarei ake e rite ana. Mā tēnei huarahi whānui ka kitea ngā tukanga pūtau me ngā tukanga ngota e pā ana ki te urupare transcriptome, kia whiwhi ai koe i ngā mōhiohio katoa e pā ana ki ngā raraunga o tō whakamātautau.
●Tautoko Whai muri i te HokongaE mārama ana mātou ki te hiranga o te noho mai, koia mātou i whakapau kaha ai ki tua atu i te otinga o te kaupapa me te wā ratonga i muri i te hoko e toru marama te roa. I tēnei wā, ka tukuna e mātou he whai i te kaupapa, he āwhina rapurongoā, me ngā wā pātai me ngā whakautu hei whakautu i ngā pātai e pā ana ki ngā hua.
Ngā Whakaritenga Tauira me te Tukunga
| Whare Pukapuka | Rautaki raupapatanga | Ngā raraunga e taunakitia ana | Mana Kounga |
| Whakarei ake i te Poly A | Illumina PE150 DNBSEQ-T7 | 6 -10 GB | Q30≥85% |
Ngā Whakaritenga Tauira:
Ngā nukleotida:
Ngā Whakaritenga Tauira Iti Rawa:
| Te nui o te konupūmā (ng/μl) | Te nui (μg) | Te parakore | Ngākau pono | |
| Whare Pukapuka Paerewa | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 He iti noa iho, kāore rānei he poke o te pūmua, o te DNA rānei e kitea ana i runga i te tiēre. | Mō ngā tipu: RIN≥4.0; Mō ngā kararehe: RIN≥4.5; 5.0≥28S/18S≥1.0; te pikinga turanga iti, kāore rānei |
| Whare Pukapuka Aronga | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 He iti noa iho, kāore rānei he poke o te pūmua, o te DNA rānei e kitea ana i runga i te tiēre. | Mō ngā tipu: RIN≥4.0; Mō ngā kararehe: RIN≥4.5; 5.0≥28S/18S≥1.0; te pikinga turanga iti, kāore rānei |
| Ngā tipu | Kararehe | Ngā Angaanga | Toto katoa | Ngā pūtau |
| Pūtake, Kākano, Putiputi rānei: 450 mg Rau, Kākano rānei: 300 mg Hua: 1.2 karamu | Ngākau, te Puku rānei: 300 mg Ngā whekau, te roro rānei: 240 mg Uaua: 450 mg Ngā wheua, ngā makawe, te kiri rānei: 1 karamu | Ngā pepeke: 6g Ngā Kōura: 300 mg | 1 ngongo | 106 ngā pūtau |
Tukunga Tauira i Manakohia
Ipu: ngongo whakapūkara 2 ml (Kāore i te tūtohutia te pepa konumohe)
Tauira tapanga: Rōpū+tāruatia hei tauira A1, A2, A3; B1, B2, B3... ...
Tukunga:
1. Hukapapa maroke: Me tākai ngā tauira ki roto i ngā putea ka tanumia ki roto i te hukapapa maroke.
2. Ngā ngongo tēpu RNA: Ka taea te whakamaroke i ngā tauira RNA i roto i te ngongo whakau RNA (hei tauira, RNAstable®) ka tukuna i te pāmahana rūma.
Ngā Whakaritenga Tauira me te Tukunga
Te hoahoa whakamātautau
Tukunga tauira
Te tango RNA
Te hanganga whare pukapuka
Te raupapatanga
Tātari raraunga
Ngā ratonga i muri i te hoko
Pūtaiao Koiora
Mō ngā tauira me te ira tohutoro e wātea ana, ko te tātaritanga BI penei:
ØTe whakahaere kounga raraunga mata
ØTe whakaritenga ira tohutoro
ØTātaritanga hanganga tuhinga
ØTe ine i te whakapuakitanga
ØTātaritanga rerekētanga o te whakaaturanga
ØTe tuhipoka me te whakarei ake i te mahi
Mō ngā tauira kāore he ira tohutoro, koinei te kōwhiringa e wātea ana:
• Te whakahaere kounga raraunga mata
•Huihuinga me te arotautanga hanganga
•Tātaritanga rerekētanga o te whakaaturanga
•Te tuhipoka me te whakarei ake i te mahi
Te Whakaritenga Ira Tohutoro
Te Mākū o te Raraunga
Te hononga tauira me te aromatawai i ngā tāruarua koiora
Ngā Ira Whakapuakitanga Rerekē (DEG)
He Whakamōhiotanga Mahi mō ngā DEG
Te Whakarei Ake i ngā Mahi a ngā DEG
Tūhurahia ngā whanaketanga i āwhinahia e ngā ratonga raupapatanga mRNA NGS eukaryotic a BMKGene mā roto i tētahi kohinga pukapuka kua whiriwhiria.
Huang, L. et al. (2023) 'Ka whakaitihia e te Triclosan me te triclocarban te kaha kakara o te ika koura mā te aukati i te mōhio ki ngā kakara, te whakararuraru i te tuku tohu kakara, me te whakararuraru i te tukatuka mōhiohio kakara',Rangahau Wai, 233, whārangi 119736. doi: 10.1016/J.WATRES.2023.119736.
Jia, LJ et al. (2023) 'Ka tahaetia e Aspergillus fumigatus te p11 tangata hei tuku i ngā phagosome kei roto he harore ki te ara kore-whakangaro',Pūtau Manaaki me te Moroiti, 31(3), wh.373-388.e10. doi: 10.1016/J.CHOM.2023.02.002.
Jin, K. et al. (2022) 'Ngā Take Whakawhiti TCP e Whai Wāhi Ana ki te Whanaketanga o te Pua o te Ma Bamboo (Dendrocalamus latiflorus Munro)',Ngā Rohe i roto i te Pūtaiao Tipu, 13, whārangi 884443. doi: 10.3389/FPLS.2022.884443/BIBTEX.
Wen, X. et al. (2022) 'Te ira Chrysanthemum lavandulifolium me te tikanga ngota i raro i ngā momo capitulum rerekē',Rangahau Māra, 9. doi: 10.1093/HR/UHAB022.
Zhang, Yujie et al. (2023) 'He nanoreactor cascade mō te whakarei ake i te rongoā sonodynamic i runga i te mate pukupuku colorectal mā te whakanui ROS synergistic me te aukati autophagy',Nano I Tēnei Rā, 49, wh. 101798. doi: 10.1016/J.NANTOD.2023.101798.
