Raupapa Roa Kore-Waehere-Illumina
Ngā Painga Ratonga
●Tātaritanga ngātahi o te mRNA me te lncRNAMā te whakakotahi i te ine i ngā tuhinga mRNA me te ako i te lncRNA me ā rātou whāinga, ka taea te whiwhi i tētahi tirohanga whānui mō te tikanga whakahaere e tūāpapa ana i te urupare pūtau.
●Tohungatanga WhānuiNeke atu i te 230,000 ngā tauira kua tukatukahia e mātou, e kapi ana i ngā tauira me ngā whāinga o ngā kaupapa. Ka kawea mai e mātou te nui o ngā pūkenga ki ia kaupapa.
●Tātaritanga ngātahi o te mRNA me te lncRNAKa whakakotahi mātou i te ine i ngā tuhinga mRNA me te ako i te lncRNA me ā rātou whāinga, kia taea ai te whiwhi tirohanga whānui ki te tikanga whakahaere e whai pānga ana ki te urupare pūtau.
●Mana Kounga PūmauKa whakatinanahia e mātou ngā pūwāhi whakahaere matua puta noa i ngā wāhanga katoa, mai i te whakarite tauira ki te whakarite whare pukapuka, te raupapatanga me te hangarau koiora. Mā tā mātou aroturukitanga tino tupato ka puta he hua kounga teitei tonu.
●He Whakarāpopototanga WhānuiKa whakamahia e mātou ngā pātengi raraunga maha hei tāpiri i ngā mahi a ngā Ira Whakapuakitanga Rerekē (DEG) me te mahi i ngā tātaritanga whakarei ake e rite ana. Mā tēnei huarahi whānui ka kitea ngā tukanga pūtau me ngā tukanga ngota e pā ana ki te urupare transcriptome, kia whiwhi ai koe i ngā mōhiohio katoa e pā ana ki ngā raraunga o tō whakamātautau.
●Tautoko Whai muri i te HokongaE mārama ana mātou ki te hiranga o te noho mai, koia mātou i whakapau kaha ai ki tua atu i te otinga o te kaupapa me te wā ratonga i muri i te hoko e toru marama te roa. I tēnei wā, ka tukuna e mātou he whai i te kaupapa, he āwhina rapurongoā, me ngā wā pātai me ngā whakautu hei whakautu i ngā pātai e pā ana ki ngā hua.
Ngā Whakaritenga Tauira me te Tukunga
| Whare Pukapuka | Papaaho | Ngā raraunga e taunakitia ana | Te Whakatau Raraunga |
| Whare pukapuka aronga rRNA kua pau | Illumina PE150 | 10-16 GB | Q30≥85% |
Ngā nukleotida:
| Te nui o te konupūmā (ng/μl) | Te nui (μg) | Te parakore | Ngākau pono |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 He iti noa iho, kāore rānei he poke o te pūmua, o te DNA rānei e kitea ana i runga i te tiēre. | RIN≥6.0; 5.0≥28S/18S≥1.0; te pikinga turanga iti, kāore rānei |
● Ngā tipu:
Pūtake, Kākano, Putiputi rānei: 450 mg
Rau, Kākano rānei: 300 mg
Hua: 1.2 karamu
● Kararehe:
Ngākau, te Puku rānei: 450 mg
Ngā whekau, te roro rānei: 240 mg
Uaua: 600 mg
Ngā wheua, ngā makawe, te kiri rānei: 1.5g
● Ngā Angaanga:
Ngā pepeke: 9g
Ngā Kōura: 450 mg
● Toto katoa:2 ngā ngongo
● Ngā pūtau: 106 ngā pūtau
● Te toto me te toto toto6 mL
Tukunga Tauira i Manakohia
Ipu: ngongo whakapūkara 2 ml (Kāore i te tūtohutia te pepa konumohe)
Tauira tapanga: Rōpū+tāruatia hei tauira A1, A2, A3; B1, B2, B3.
Tukunga:
1. Hukapapa maroke: Me tākai ngā tauira ki roto i ngā putea ka tanumia ki roto i te hukapapa maroke.
2. Ngā ngongo RNA-pūmau: Ka taea te whakamaroke i ngā tauira RNA i roto i te ngongo RNA-pūmau (hei tauira, RNA-pūmau®) ka tukuna i te pāmahana rūma.
Rere Mahi Ratonga
Te hoahoa whakamātautau
Tukunga tauira
Te tango RNA
Te hanganga whare pukapuka
Te raupapatanga
Tātari raraunga
Ngā ratonga i muri i te hoko
Pūtaiao Koiora
- Raraunga mata
- Te Whakatau Raraunga
- Te whakarārangi ira
- Hanganga ira (Te honohono rerekē, te arotautanga hanganga ira me te matapae ira hou)
- Te ine i te whakaaturanga ira
- Tātaritanga rerekētanga o te whakaaturanga
- Te tuhipoka me te whakarei ake i te DEG + Ngā ira ūnga lncRNA kua whakaatuhia rerekē
- Te tautuhi tuhinga
- Te tautuhi i te lncRNA (te tiaki i te lncRNA me te lncRNA e mōhiotia ana)
- Te matapae i ngā ira ūnga lncRNA
- Te ine i te whakaaturanga lncRNA
- Tātaritanga tahi me ngā raraunga mRNA
Te tātari i te Whakapuakitanga Ira Rerekē (DEG)
Te ine i te whakaaturanga lncRNA – te whakarōpūtanga
Te whakarei ake i ngā ira ūnga lncRNA
Tātaritanga tūranga hononga mRNA me te lncRNA – Kauwhata Circos (ko te porowhita waenga ko te mRNA, ko te porowhita o roto ko te lncRNA)
Tūhurahia ngā whanaketanga i āwhinahia e ngā ratonga raupapatanga lncRNA a BMKGene mā roto i tētahi kohinga pukapuka kua whiriwhiria.
Ji, H. et al. (2020) 'Te tautuhi, te matapae mahi, me te manatoko matua o te lncRNA o ngā lncRNA e pā ana ki te ahotea makariri i roto i te ate o ngā kiore',Ngā Pūrongo Pūtaiao2020 10:1, 10(1), whārangi 1–14. doi: 10.1038/s41598-020-57451-7.
Jia, Z. et al. (2021) 'E whakaatu ana te Tātaritanga Whakawhitiwhiti Whakauru i te Tikanga Ārai Mate mō te Momo Kapā Noa Ātete CyHV-3',Ngā Rohe i roto i te Pūtaiao Ārai Mate, 12, wh. 687151. doi: 10.3389/FIMMU.2021.687151/BIBTEX.
Wang, XJ et al. (2022) 'Te Whakatakotoranga Matua i runga i te Whakaurunga Maha-Omics o ngā Whatunga Whakahaere RNA Endogenous Whakataetae i roto i te Matepukupuku Pūkahukahu Pūtau Iti: Ngā Āhuatanga Ngota me ngā Kaitatau Rongoā',Ngā Rohe i roto i te Oncology, 12, wh. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xiao, L. et al. (2020) 'Te wetewete ira o te whatunga whakaputanga ira e ahu mai ana i te whakaahua i roto i te Populus',Te Hautaka Hangarau Koiora Tipu, 18(4), wh. 1015–1026. doi: 10.1111/PBI.13270.
Zheng, H. et al. (2022) 'He Whatunga Whakahaere Ao mō te Whakapuakitanga Ira Kore-Whakahaere me te Tohu Pūmua Tauhou i roto i ngā Pūtau Ārai Mate i roto i te Taiao Moroiti o te Mate o Graves me te Mate Tairoidi o Hashimoto',Ngā Rohe i roto i te Pūtaiao Ārai Mate, 13, wh. 879824. doi: 10.3389/FIMMU.2022.879824/BIBTEX.
