mRNA-seq (NGS) me te ira tohutoro
He taputapu paerewa te RNA-seq puta noa i ngā pūtaiao koiora me ngā pūtaiao hua whenua, e hono ana i te āputa i waenga i ngā ira me ngā proteome. Ko tōna kaha kei te kimi i ngā tuhinga hou me te ine i ō rātou whakaaturanga i roto i te whakamātautau kotahi. He whānuitia te whakamahinga mō ngā rangahau whakatairite i te tuhinga, e whakamārama ana i ngā ira e pā ana ki ngā āhuatanga rerekē, pērā i te whakatairite i ngā mutants ki ngā momo-mohoao, te whakaatu rānei i te whakaaturanga ira i raro i ngā āhuatanga motuhake. Ka whakauruhia e te BMKCloud mRNA (Tohutoro) APP te ine whakaaturanga, te tātari whakaaturanga rerekētanga (DEG), me ngā tātari hanganga raupapatanga ki roto i te paipa bioinformatics mRNA-seq (NGS) me te whakakotahi i ngā kaha o ngā pūmanawa rite, e whakarite ana i te watea me te ngāwari ki te whakamahi. Ka taea e ngā kaiwhakamahi te tuku ake i ā rātou raraunga RNA-seq ki te kapua, kei reira te Taupānga e tuku ana i tētahi otinga tātari bioinformatic whānui, kotahi-mutu. Hei tāpiri, ka whakatairanga i te wheako kiritaki, e tuku ana i ngā mahi whaiaro kua whakaritea ki ngā hiahia motuhake o ngā kaiwhakamahi. Ka taea e ngā kaiwhakamahi te whakatakoto tawhā me te tuku i te kaupapa paipa mā rātou anō, tirohia te pūrongo tauwhitiwhiti, tiro i ngā raraunga/hoahoa me te keri raraunga katoa, pērā i: te kōwhiringa ira ūnga, te whakarōpū mahi, te hoahoa, me ētahi atu.
Pūhara:Illumina, MGI
Rautaki:RNA-Seq
Tahora: Ngā raraunga kua oti te whakarite, kua ma.
Momo whare pukapuka:kāore he aho, he aho tuatahi, he aho tuarua rānei
Te roa o te pānui:150 bp
Momo kōnae:*.fastq, *.fq, *.fastq.gz, *.fq.gz rānei. Kahono aunoa i ngā kōnae .fastq kia rite ki ō rātou ingoa kōnae,hei tauira, kua honoa a *_1.fastq ki a *._2.fastq.
Te maha o ngā tauira:Kāore he herenga mō te mahao ngā tauira, engari ka piki ake te wā tātari i te mea ka nui haere te maha o ngāka tipu haere ngā tauira.
Te nui o ngā raraunga e taunakitia ana:6G mō ia tauira
