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Chromatin Immunoprecipitation Sequencing (ChIP-seq)

Chromatin Immunoprecipitation (CHIP) is a technique that leverages antibodies to selectively enrich DNA-binding proteins and their corresponding genomics targets. Its integration with NGS enables the genome-wide profiling of DNA targets associated with histone modification, transcription factors, and other DNA-binding proteins. This dynamic approach enables comparisons of binding sites across diverse cell types, tissues, or conditions. ChIP-Seq’s applications span from studying transcriptional regulation and developmental pathways to elucidating disease mechanisms, making it an indispensable tool for understanding genomic regulation landscapes and advancing therapeutic insights.

Platform: Illumina NovaSeq


Service Details

Bioinformatics

Demo Results

Service Advantages

●  Advanced Bioinformatic Analysis and Comprehensive Annotation: We use multiple databases to functionally annotate the genes associated with regions of protein-DNA binding, providing insights on the cellular and molecular processes underlying the interaction.

●  Post-Sales Support: Our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.

●  Extensive Experience: With a track record of successfully completing numerous ChIP-Seq projects, our company brings over a decade of expertise to the table. Our highly skilled analysis team, coupled with comprehensive content and post-sales support, ensures the success of your projects.

●  Rigorous Quality Control: We implement core control points across all stages, from sample and library preparation to sequencing and bioinformatics. This meticulous monitoring ensures the delivery of consistently high-quality results.

Service Specifications

Library

Sequencing Strategy

Recommended data output

Quality control

Purified DNA after Immunoprecipitation

Illumina PE150

10Gb

Q30≥85%

Bisulfite conversion >99%

MspI cutting efficiency > 95%

Sample Requirements

Total amount: ≥10 ng

Fragment size distribution: 100-750 bps

Service Work Flow

sample delivery

Sample delivery

Library Preparation

Library construction

Sequencing

Sequencing

Data analysis

Data analysis

After sale Services

After-sale services


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    Includes the following analysis:

    ● Raw data quality control

    ● Peak calling based on mapping to reference genome

    ● Annotation of peak-associated genes

    ● Motif analysis: identification of transcription factor binding sites (TFBS)

    ● Differential Peak Analysis and annotation

    Evaluation of enrichment near Transcription Starting Sites (TSSs)

     

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    Genome-wide distribution of CHIP peaks

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    Classification of peak regions

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    Functional enrichment of peak-related genes (KEGG)

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