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Chromatin Immunoprecipitation Sequencing (ChIP-seq)

ChIP-Seq provides genome-wide profiling of DNA targets for histone modification, transcription factors, and other DNA-associated proteins. It combines the selectivity of chromatin immuno-precipitation (ChIP) to recover specific protein-DNA complexes, with the power of next-generation sequencing (NGS) for high-throughput sequencing of the recovered DNA. Additionally, because the protein-DNA complexes are recovered from living cells, binding sites can be compared in different cell types and tissues, or under different conditions. Applications range from transcriptional regulation to developmental pathways to disease mechanisms and beyond.

Platform: Illumina NovaSeq Platform

Service Details


Demo Results

Service Advantages

●  Platform: Illumina NovaSeq Platform, PE150

● Library type: 100-500bp insert DNA library (depends on peak distribution)

● Sequencing strategy: Paired-End 150 bp

● Recommend data output: 6 Gb raw data/sample.

Sample Requirements

DNA quantity: ≥ 70 ng, main peak of 100 to 500 bp

Sample volume: ≥ 10 μl

OD260/280 = 1.8 to 2.0 without degradation or RNA contamination

Experimental workflow


Service Work Flow

sample delivery

Sample delivery

Library Preparation

Library construction



Data analysis

Data analysis

After sale Services

After-sale services

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  • 表观-01

    1. Peak enrichment distribution



    2. MA plot of differential peak



    3. KEGG annotation



    4. GO classification


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