● Platform: Illumina NovaSeq Platform, PE150
● Library type: 100-500bp insert DNA library (depends on peak distribution)
● Sequencing strategy: Paired-End 150 bp
● Recommend data output: 6 Gb raw data/sample.
DNA quantity: ≥ 70 ng, main peak of 100 to 500 bp
Sample volume: ≥ 10 μl
OD260/280 = 1.8 to 2.0 without degradation or RNA contamination
1. Peak enrichment distribution
2. MA plot of differential peak
3. KEGG annotation
4. GO classification