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I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)

I-Chromatin Immunoprecipitation Sequencing (ChIP-seq) Isithombe Esifakiwe
  • I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)
  • I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)

I-ChIP-Seq inikeza iphrofayili ebanzi yofuzo lwezinjongo ze-DNA zokuguqulwa kwe-histone, izici zokuloba, namanye amaprotheni ahlobene ne-DNA.Ihlanganisa ukukhethwa kwe-chromatin immuno-precipitation (ChIP) ukuze kubuyiselwe ama-protein-DNA complexes athile, namandla okulandelana kwesizukulwane esilandelayo (NGS) okulandelana okuphezulu komphumela we-DNA etholiwe.Ukwengeza, ngenxa yokuthi ama-protein-DNA complexes atholakala kumangqamuzana aphilayo, izingosi ezibophayo zingaqhathaniswa ngezinhlobo zamangqamuzana nezicubu ezihlukene, noma ngaphansi kwezimo ezihlukene.Izicelo zisukela ekulawuleni okulotshiweyo kuye ezindleleni zokuthuthuka eziya ezindleleni zezifo nangale kwalokho.

Ipulatifomu: I-Illumina NovaSeq Platform


Imininingwane Yesevisi

I-Bioinformatics

Imiphumela yedemo

Izinzuzo Zesevisi

● I-Platform: I-Illumina NovaSeq Platform, PE150

● Uhlobo lwelabhulali: 100-500bp faka umtapo wezincwadi we-DNA (kuncike ekusabalaliseni okuphezulu)

● Isu lokulandelanisa: I-Painted-End 150 bp

● Ncoma okuphumayo kwedatha: 6 Gb idatha/isampula eluhlaza.

Izidingo Zesampula

Ubuningi be-DNA: ≥ 70 ng, ukuphakama okuyinhloko kwe-100 kuya ku-500 bp

Ivolumu yesampula: ≥ 10 μl

OD260/280 = 1.8 kuya ku-2.0 ngaphandle kokucekelwa phansi noma ukungcoliswa kwe-RNA

Ukugeleza komsebenzi wokuhlola

图片1

Ukugeleza Komsebenzi Wesevisi

ukulethwa kwesampula

Ukulethwa kwesampula

Ukulungiselela Umtapowolwazi

Ukwakhiwa komtapo wolwazi

Ukulandelanisa

Ukulandelanisa

Ukuhlaziywa kwedatha

Ukuhlaziywa kwedatha

Ngemva kokudayiswa Services

Amasevisi angemuva kokuthengisa

  • Okwedlule:
  • Olandelayo:

    • 表观-01

    1. Ukusatshalaliswa kokunothisa okuphezulu

    官网图1

     

    2. Isakhiwo se-MA sesiqongo esihlukile

    官网图2

     

    3.KEGG isichasiselo

    官网图3

     

    4. GO ngezigaba

    官网图4

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