Umhlinzeki Nabakhiqizi Abahle Kakhulu Be-Chromatin Immunoprecipitation Sequencing (ChIP-seq)

Ukulandelana Kwe-Chromatin Immunoprecipitation (ChIP-seq)

Isithombe Esifakiwe se-Chromatin Immunoprecipitation Sequencing (ChIP-seq)

I-Chromatin Immunoprecipitation (CHIP) iyindlela esebenzisa ama-antibodies ukuze ikhethe ngokukhethekile amaprotheni ahlanganisa i-DNA kanye nezinhloso zawo ze-genomics. Ukuhlanganiswa kwayo ne-NGS kwenza kube lula ukuqoshwa kwe-genome yonke kwezinhloso ze-DNA ezihlobene nokuguqulwa kwe-histone, izici zokubhala, kanye namanye amaprotheni ahlanganisa i-DNA. Le ndlela enamandla ivumela ukuqhathaniswa kwezindawo zokubopha kuzo zonke izinhlobo zamaseli, izicubu, noma izimo. Ukusetshenziswa kwe-ChIP-Seq kuqala ekutadisheni ukulawulwa kokubhala kanye nezindlela zokuthuthukiswa kuya ekuchazeni izindlela zesifo, okwenza kube ithuluzi elibalulekile lokuqonda izindawo zokulawulwa kokuzalwa kanye nokuthuthukisa ukuqonda kokwelapha.

Ipulatifomu: Illumina NovaSeq

Xhumana nathi

Imininingwane Yesevisi

I-Bioinformatics

Imiphumela Yedemo

Izinzuzo Zesevisi

●Ukuhlaziywa Okuthuthukisiwe Kwe-Bioinformatic kanye Nezichasiselo Eziphelele:Sisebenzisa izizindalwazi eziningi ukuze sichaze ngokusebenzayo izakhi zofuzo ezihlotshaniswa nezindawo zokubopha amaprotheni-DNA, sinikeze ukuqonda ngezinqubo zamaseli kanye nama-molecule ezisekela ukusebenzisana.

●Usekelo Lwangemva Kokuthengisa:Ukuzibophezela kwethu kudlulela ngale kokuqedwa kwephrojekthi ngesikhathi senkonzo yezinyanga ezintathu ngemuva kokuthengisa. Phakathi nalesi sikhathi, sinikeza ukulandelwa kwephrojekthi, usizo lokuxazulula izinkinga, kanye nezikhathi ze-Q&A ukuze kuxazululwe noma yimiphi imibuzo ehlobene nemiphumela.

●Okuhlangenwe nakho Okubanzi:Njengoba inerekhodi elihle lokuqeda ngempumelelo amaphrojekthi amaningi e-ChIP-Seq, inkampani yethu iletha ubuchwepheshe obungaphezu kweminyaka eyishumi etafuleni. Ithimba lethu lokuhlaziya elinekhono eliphezulu, kanye nokuqukethwe okuphelele kanye nokusekelwa kwangemva kokuthengisa, liqinisekisa impumelelo yamaphrojekthi akho.

● Ukulawulwa Kwekhwalithi Okuqinile: Sisebenzisa amaphuzu okulawula ayisisekelo kuzo zonke izigaba, kusukela ekulungiseleleni amasampula kanye nomtapo wolwazi kuya ekulandeleni nasekubhaleni ulwazi nge-bioinformatics. Lokhu kuqapha ngokucophelela kuqinisekisa ukulethwa kwemiphumela esezingeni eliphezulu njalo.

Imininingwane Yesevisi

Umtapo Wolwazi	Isu Lokulandelana	Ukukhishwa kwedatha okunconyiwe	Ikhwalithi yokulawula
I-DNA ehlanziwe ngemuva kwe-Immunoprecipitation	I-Illumina PE150	10Gb	Q30≥85%