Sahaminta qaababka muujinta hiddaha ee goobta asalka ah ee noolaha ayaa muhiim u ah fahamka noocyada iyo shaqooyinka unugyadiisa.Si kastaba ha ahaatee, hababka falanqaynta transcriptome ee hadda jira waxay leeyihiin xaddidaadyo sida wax-soo-saar hooseeya ama xallin aan ku filnayn.BMKMANU S1000 Spatial Chip, oo ay soo saartay BMKMANU, waxay ku ogaan kartaa macluumaadka muujinta hidda-wadaha goobta-gudaha qaybaha unugyada ee xallinta unugyada hoose, taas oo awood u siinaysa tafsiir kooban ee qaab dhismeedka unugyada.
U diyaargarow inaad khibrad u yeelatid alaabtayada cusub!Si loo dabaaldego siideyntiisa, waxaan bixineynaa mid gaar ahIibso Afar Hal Bilaashdallacaad waqti xaddidan oo keliya.
Qaybaha Alaabta
BMKMANU S1000 chip spatial chip iyo xirmada reagent ee u dhigma waxaa ka mid ah:
1) Xirmada hagaajinta unugyada, oo ay ku jiraan reagents tayaynta unugyada (sahaminta wakhtiga ugu fiican ee nudaha nudaha).
2) Qalabka Muujinta Hiddaha, oo ka kooban reagen-ka qabashada mRNA ee jeexjeexyada unugyada diyaarinta iyo isku xigxiga maktabadda.
3) Qalabka Bilawga: Waxa ku jira adabtarada heerkulbeegga iyo kala-soocida birlabeedka.
Mabda'a Farsamada
Chipku wuxuu ku salaysan yahay micropores iyo microbeads, mRNA waxaa lagu qabsan karaa goobta iyada oo loo marayo Oligo on microbeads.Oligogu wuxuu ka kooban yahay afar qaybood: Read1, Spatial Barcode, UMI, iyo Poly(dT)VN.Ka dib marka nudaha lagu xidho jajabka, mRNA ayaa laga sii daayaa unugga iyada oo la sii daayo enzyme permeabilization.Maadaama inta badan mRNA 3' darafyada ay leeyihiin dabada Poly-A, waxaa qabta Oligo oo wata Poly(dT) VN.RT-PCR iyo cDNA ka dib, diyaarinta iyo isku xigxiga maktabadda, baafinta booska boosaska waxaa lagu gaaraa Barcode Spatial.Tani waxay u oggolaanaysaa falanqaynta muujinta hidde-sidaha ee meelaha bannaan iyo kala duwanaanta unugyada.
Faa'iidooyinka Alaabta
Codsiyada
Waxaa lagu dabaqi karaa inta badan aagagga cilmi-baarista bayoolojiga iyo caafimaadka, oo ay ku jiraan buro, cudur, immunology, iyo bayoolaji korriinka, ka caawinta in la gaaro horumarro cusub oo dhinacyadan ah oo leh falanqaynta muujinta booska ee xallinta unugyada hoose.
●Buro iyo Cudur:
Heterogeneity meel bannaan iyo microenvironment ee burooyinka iyo cudurada
Bilawga iyo horumarinta burooyinka iyo cudurrada
Jawaabta daaweynta burooyinka iyo cudurrada
●Bayooloji Horumarineed
Spatiotemporal atlas ee xubnaha
Hababka nidaaminta hidda-wadaha inta lagu jiro horumarka
●Jawaabta Cadaadiska
Ka jawaab celinta diiqada noolaha
Jawaabta walaaca Abiotic
●Immunology
Jawaabta difaaca jirka ee xubinta taranka
Hababka difaaca ee burooyinka iyo cudurrada
Pathogenesis ee cudurada autoimmune
● Falanqaynta caabbinta daroogada
Farsamooyinka caabbinta daroogada
Cilmi-baarista iyo horumarinta dawooyinka cusub
Kiisaska iyo Xogta Demo
BMKMANU S1000 waxa lagu xaqiijiyay waxqabadka boqolaal xaaladood oo noocyo kala duwan ah.
